Octaviani, Devi Intan Dyah Ayu (2018) Pengaruh Pemberian Ekstrak Etanol Daun Kemangi (Ocimum canum Sims.) Terhadap Gambaran Profil Protein dan Ekspresi Tryptase pada tikus (Rattus norvegicus) Model Asma. Sarjana thesis, Universitas Brawijaya.
Abstract
Asma merupakan inflamasi kronik saluran napas dengan tanda utama berupa obstruksi saluran napas reversibel akibat kontraksi otot polos bronkus, hipersekresi mukus, dan edema mukosa. Pemberian ovalbumin (OVA) dapat menyebabkan asma dan diperparah dengan lipopolisakarida (LPS). Pemberian ekstrak daun kemangi (Ocimum canum Sims.) digunakan sebagai terapi penyakit asma karena memiliki kandungan antioksidan dan antiinflamasi sehingga dapat digunakan untuk terapi alternatif asma. Tujuan dari penelitian ini untuk mengetahui pengaruh terapi ekstrak etanol daun kemangi (Ocimum canum Sims.) terhadap ekspresi Tryptase dan gambaran profil protein organ paru tikus putih model asma. Penelitian ini dilakukan dengan menggunakan lima kelompok tikus, yaitu kelompok kontrol negatif, kontrol positif yang diinduksi OVA dan LPS dan kelompok P1, P2, P3 adalah tikus model asma yang berturut – turut mendapat terapi ekstrak etanol daun kemangi (Ocimum canum Sims.) dengan dosis 600 mg/kg BB, 900 mg/kg BB dan 1200 mg/kg BB. Parameter yang diamati adalah ekspresi Tryptase yang diamati dengan imunohistokimia dan profil protein yang diamati dengan metode SDS-PAGE. Hasil penelitian menunjukkan bahwa pemberian terapi ekstrak daun kemangi (Ocimum canum Sims.) secara signifikan (P<0,05) menurunkan ekspresi tryptase dan memperbaiki profil pita protein. Dosis 1200 mg/kgBB merupakan dosis terbaik. Kesimpulan, ekstrak etanol daun kemangi (Ocimum canum Sims.) dapat dijadikan salah satu alternatif pengobatan asma.
English Abstract
Asthma is chronical inflammation on respiratory tract that signed with the obstruction on reversible respiratory tract due to the contraction of bronchial muscle, hyper secretion of mucus, and edema mucosa. The adding of ovalbumin (OVA) caused asthma and aggravated with the adding of lipopolysaccharide (LPS). The adding of Ocinum canum Sims. was used as therapy for asthma due to its content of antioxidant and anti inflammation, this extract could be used as alternative therapy for asthma. This study aimed to understand the effect of therapy by using etanol extract of Ocinum canum Sims. against the Tryptase expression and the protein profile on rat’s lungs which the asthma model. This study was conducted by using five groups of white rat, they were the group of negative control, positive control that inducted with OVA and LPS and the group of P1, P2, and P3were the same rat model that got the same therapy of etanol extract of Ocimum canum Sims. with doses of 600 mm/kg BW, 900 mm/kg BW, and 1200 mm/kg BW. The observed parameters were Tryptase expression that observed by using immunohistochemistry technique and profile protein that observed by using SDS-PAGE methods. The result of study showed that therapy by using etanol extract of Ocinum canum Sims. decreased significantly the tryptase expression (P <0.05) and repaired the profile protein and therapy at the doses of 1200 mg/kg BW was the best doses. In conclusion etanol extract of Ocinum canum Sims. could be one of alternative therapy for asthma.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FKH/2018/133/051808853 |
| Uncontrolled Keywords: | Ocimum canum Sims., Asma, Ekspresi Tryptase, profil protein.-Ocinum canum Sims, Asthma, Tryptase expression, Protein profile |
| Subjects: | 600 Technology (Applied sciences) > 615 Pharmacology and therapeutics > 615.3 Organics drugs > 615.32 Drugs derived from plants and mikroorganisms > 615.323 96 Drugs derived from spesific plant (Lamiales) |
| Divisions: | Fakultas Kedokteran Hewan > Kedokteran Hewan |
| Depositing User: | soegeng sugeng |
| Date Deposited: | 21 Feb 2019 02:26 |
| Last Modified: | 22 Oct 2021 03:28 |
| URI: | http://repository.ub.ac.id/id/eprint/161395 |
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