Keragaman Genetik Lutung Jawa (Trachypithecus auratus Geoffroy 1812) di Pusat Rehabilitasi Javan Langur Conservation Program Berdasarkan Gen Cyt b

Sakti, MaurissaAndhitaEka (2011) Keragaman Genetik Lutung Jawa (Trachypithecus auratus Geoffroy 1812) di Pusat Rehabilitasi Javan Langur Conservation Program Berdasarkan Gen Cyt b. Magister thesis, Universitas Brawijaya.

Abstract

Lutung Jawa merupakan satwa endemik Pulau Jawa yang terdistribusi mulai dari Pulau Jawa hingga Lombok. Populasi spesies ini menurun akibat perdagangan dan perburuan ilegal. Javan Langur Conservation (JLC) Program merupakan salah satu organisasi yang berupaya mengkonservasi populasi satwa ini. Lutung Jawa yang ada di pusat rehabilitasi berasal dari penyitaan dari pemilik ilegal serta hasil penyerahan masyarakat. Lutung Jawa yang direhabilitasi akan dire-introduksi pada habitat alami. Namun, hingga sekarang data yang di JLC terbatas pada asal individu Lutung Jawa dan tidak terdapat data molecular Lutung Jawa yang penting untuk mengetahui keragaman genetiknya. Penelitian ini bertujuan untuk mengetahui keragaman genetik Lutung Jawa yang ada di JLC Program, berdasarkan gen cyt b . Pemilihan gen cyt b karena variasi sekuen untuk studi filogeni hingga level subspesies. Tiga belas sampel darah Lutung diperoleh di JLC. Gen cyt b parsial (± 500 bp) dari panjang 1140 bp berhasil diamplifikasi dengan PCR menggunakan primer spesifik dan dilanjutkan dengan sekuensing gen cyt b . Hasil penelitian ini menunjukkan bahwa keragaman genetik 13 sekuen gen cyt b Lutung sebesar 0,016. Dari 12 sekuen gen cyt b Lutung Jawa ( Trachypi cus auratus ) terdiri atas 3 pasang haplotype homolog dan 6 haplotype berbeda. Hasil rekonstruksi pohon filogeni menunjukkan bahwa Lutung Jawa tidak berkelompok sesuai lokasi geografinya. Hasil analisis lebih lanjut menunjukkan bahwa sedang terjadi pembentukan subspesies baru pada kelompok T. auratus auratus .

English Abstract

Ebony leaf monkeys (javan langur) is an endemic animal to Javan island, which is distribute from Java to Lombok island. population at spesies has declined due to illegal hunting and trading. Javan Langur Conservation (JLC) Program are organization that tried to conserve javan langur population. Javan langur in this rehabilitation center was collected from confiscated of illegal owner and transferred by society. Rehabilitated javan langur will be re-introduces into its natural habitat. However, JLC doesn`t have molecular data of javan langur which is important to understand its genetic diversity. data is avaible to predict succesfility javan langur adapt to new environmental. aim of study is to determine genetic diversity of javan langur in rehabilitation center (JLC), based on cyt b gene. Thirteen blood samples were acquired from javan langur in JLC. A partial sequence (573 bp long fragment) of whole cyt b gene (1140 bp) was amplified by PCR, using specific primer, n followed by sequencing. Result from this study showed that genetic diversity of 13 sequen of cyt b gene javan langur are 0,016. From 12 sequence cyt b gene of javan langur consist of 3 pair homolog haplotype and 9 different haplotypes. phylogenetic tree showed that javan langurs are not well distributed based on geographic. Fur rmore, results suggest that re are process at establish new subspecies of T auratus auratus.

Item Type:	Thesis (Magister)
Identification Number:	TES/639.979 862/SAK/k/041103895
Subjects:	600 Technology (Applied sciences) > 639 Hunting, fishing & conservation > 639.9 Conservation of biological resources
Divisions:	S2/S3 > Magister Matematika, Fakultas MIPA
Depositing User:	Endro Setyobudi
Date Deposited:	28 Sep 2011 10:44
Last Modified:	30 Mar 2022 07:45
URI:	http://repository.ub.ac.id/id/eprint/159104

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