Purwoistri, Rita Fitria (2012) Kualitas dan Membran Spermatozoa Sapi setelah Sexing dengan Gradien Albumin (Putih Telur) Menggunakan Pengencer Andromed dan CEP-2 Ditambah Kuning Telur 10%. Magister thesis, Universitas Brawijaya.
Abstract
Penelitian ini bertujuan untuk mengetahui kualitas dan membran spermatozoa setelah sexing dengan gradien albumin (putih telur) menggunakan pengencer andromed dan Cauda Epididymal Plasma 2 (CEP-2) ditambah kuning telur 10%. Pengamatan kualitas spermatozoa meliputi motilitas, viabilitas, abnormalitas, konsentrasi dan total spermatozoa motil. Sedangkan pengamatan membran spermatozoa meliputi spermatozoa yang memiliki integritas membran baik, belum kapasitasi, kapasitasi dan reaksi akrosom spermatozoa. Pengamatan integritas membran menggunakan HOST ( Hypoosmotic Swelling Test ), pengamatan kapasitasi dan reaksi akrosom spermatozoa menggunakan pewarna fluoresen CTC ( Chlortetracycline ). Hasil penelitian menunjukkan pengencer andromed dan CEP-2 ditambah kuning telur 10% dapat menjaga penurunan kualitas spermatozoa. Motilitas, viabilitas, konsentrasi dan total spermatozoa motil dijaga tetap tinggi, sedangkan abnormalitas dijaga tetap rendah. Pengencer andromed dan CEP-2 ditambah kuning telur 10% dapat melindungi membran spermatozoa. Pengencer andromed dan CEP-2 ditambah kuning telur 10% dapat mengurangi penurunan spermatozoa yang memiliki integritas membran baik dan spermatozoa belum kapasitasi, sedangkan kapasitasi dan reaksi akrosom dijaga tetap rendah. Pengencer CEP-2 ditambah kuning telur 10% dapat menjaga kualitas dan membran spermatozoa lebih baik daripada pengencer andromed.
English Abstract
objective of this study was to determine quality and membrane of sperm after sexing with gradient albumin (egg white) using andromed and Cauda Epididymal Plasma 2 (CEP-2) supplemented with 10% egg yolk. observations of sperm quality included motility, viability, abnormality, concentration, and total motile sperm. Whereas observations of sperm membrane included membrane integrity, uncapacitated, capacitated and acrosome reacted. Albumin gradient was made by mixing egg white with andromed or CEP-2 supplemented with 10% egg yolk resulting in a concentration of egg whites 10%, 30%, and 50%. Membrane integrity was observed using HOST (Hypoosmotic Swelling Test), while uncapacitated, capacitated and acrosome reacted sperm were observed using CTC (Chlortetracycline) staining. results showed that andromed and CEP-2 supplemented with 10% egg yolk could reduce decline in sperm quality. Motility, viability, concentration and total motile sperm could be kept high, whereas abnormality could be kept low. Andromed and CEP-2 supplemented with 10% egg yolk could protected sperm membrane. Andromed and CEP-2 supplemented with 10% egg yolk could protect membrane integrity, uncapacitated, capacitated and acrosome reacted sperm. Andromed and CEP-2 supplement with 10% egg yolk could reduce demage of membrane integrity and uncapacitated sperm, whereas capacitated and acrosome reacted sperm could be kept low. CEP-2 supplemented with 10% egg yolk keep quality and membrane of sperm is good more than diluter andromed.
| Item Type: | Thesis (Magister) |
|---|---|
| Identification Number: | TES/636.082 4/PUR/k/041205675 |
| Subjects: | 600 Technology (Applied sciences) > 636 Animal husbandry > 636.08 Specific topics in animal husbandry |
| Divisions: | S2/S3 > Magister Biologi, Fakultas MIPA |
| Depositing User: | Endro Setyobudi |
| Date Deposited: | 04 Jan 2013 11:24 |
| Last Modified: | 30 Mar 2022 07:32 |
| URI: | http://repository.ub.ac.id/id/eprint/158980 |
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