Indriani (2013) Kualitas Spermatozoa Sapi Limousin pada Suhu 5˚C dengan Metode Penyimpanan Waterjacket dan Free Waterjacket. Magister thesis, Universitas Brawijaya.
Abstract
I nseminasi buatan (IB) merupakan bioteknologi reproduksi yang bisa digunakan untuk meningkatkan produktivitas ternak . Berhasilnya program IB sangat tergantung pada kualitas semen yang diejakulasikan seekor pejantan. Semen sesudah pengenceran harus segera disimpan apabila tidak langsung digunakan, salah satunya yaitu disimpan pada suhu 5 0 C. Penyimpanan semen di lemari es pada suhu 5 0 C dapat dilakukan dengan metode water jacket dan metode free water jacket . Water jacket yaitu penambahan air pada beker gelas sebagai tempat menaruh tabung reaksi yang sudah berisi semen sesudah diencerkan. Metode free water jacket yaitu penyimpanan semen pada refrigerator yang diletakkan secara bebas tanpa penambahan media air. Penelitian ini bertujuan untuk mengetahui pengaruh metode simpan yang berbeda yaitu metode water jacket dan metode free water jacket t erhadap kualitas spermatozoa sapi Limousin setelah disimpan pada suhu 5 0 C. Semen sapi yang memenuhi syarat, diencerkan dengan pengencer CEP-2 ditambah kuning telur 10%. Hasil penyimpanan diamati kualitasnya yang meliputi motilitas, viabilitas, abnormalitas, total spermatozoa motil. Sedangkan pengamatanuji lanjut meliputi integritas membran menggunakan hypoosmotic swelling test (HOST), pengamatan kapasitasi dan reaksi akrosom menggunakan chlortetracycline (CTC). Hasil penelitian menunjukan metode free water jacket lebih baik dalam mempertahankan kualitas spermatozoa dengan motilitas (28.75%), viabilitas (66.92), abnormalitas (20.17%), total spermatozoa motil (85.07 juta/ml), integritas membran utuh (65.38%), spermatozoa non kapasitasi (55.15%), terkapasitasi (37.57%) dan reaksi akrosom (7.28%) setelah penyimpanan 48 jam pada suhu 5 0 C.
English Abstract
Artificial insemination (AI) is a reproductive biotechnology can be used to increase productivity of livestock. Successful IB program is highly dependent on quality of semen ejaculated a stud. seme should be stored after dilution if not immediately used, one of which is kept at a temperature of 5 0 C. Cement storage in a refrigerator at a temperature of 5 0 C can be done with a water jacket method and method of free water jacket. Water jacket is addition of water in a glass beaker as a place to put test tubes already containing diluted semen after. Method of free water jacket on cement storage refrigerator placed freely without addition of water media. This study aimed to determine effect of different methods that save water jacket method and method of free water jacket on sperm quality Limousin cattle after being stored at a temperature of 5 0 C. Eligible cattle semen, diluted with CEP-2 diluent plus 10% egg yolk. Storage results were observed covering quality motile, viability, abnormality, tital motile spermatozoa. While pengamatanuji fur r including membrane integrity using hypoosmotic swelling test (HOST), observations of capacitation and acrosome reaction using chlortetracycline (CTC). results showed method of free water jacket better in maintaining quality of spermatozoa with motility (28.75%), viability (66.92), abnormality (20:17%), total motile spermatozoa (85.07 million/ml), intact membrane integrity (65.38%), spermatozoa non capacitation (55.15%), terkapasitasi (37.57%) and acrosome reaction (7:28%) after 48 hours of storage at a temperature of 5 0 C.
| Item Type: | Thesis (Magister) |
|---|---|
| Identification Number: | TES/636.082 4/IND/k/041305531 |
| Subjects: | 600 Technology (Applied sciences) > 636 Animal husbandry > 636.08 Specific topics in animal husbandry |
| Divisions: | S2/S3 > Magister Ilmu Ternak, Fakultas Peternakan |
| Depositing User: | Endro Setyobudi |
| Date Deposited: | 08 Jan 2014 09:40 |
| Last Modified: | 08 Jan 2014 09:40 |
| URI: | http://repository.ub.ac.id/id/eprint/158978 |
Actions (login required)
 |
View Item |