Primardianti, Antiek (2014) Uji Imunogenitas Protein Rekombinan 38 kDa Mycobacterium tuberculosis Galur Malang melalui Sintesis Interleukin-2 dan Interferon gamma pada Kultur PBMC. Magister thesis, Universitas Brawijaya.
Abstract
Latar Belakang .Tuberkulosis (TB) yang disebabkan oleh Mycobacterium tuberculosis (Mtb), merupakan salah satu penyebab kematian utama. WHO (2012), menempatkan Indonesia sebagai penyumbang TB nomor 4 dunia. Respons imun utama dalam patogenesis TB adalah Cell mediated immunity, diperankan limfosit T. Interleukin-2 adalah faktor pertumbuhan limfosit T. Interferon G amma adalah sitokin kunci pengendalian infeksi Mtb yang , diproduksi limfosit T. Strategi vaksinasi yang efektif adalah yang dapat menstimulasi respons limfosit T untuk menghasilkan sitokin-sitokin. Protein 38 kDa Mtb potensial untuk pengembangan vaksin TB karena memiliki epitop untuk limfosit T. Tujuan . Menentukan bahwa protein rekombinan 38 kDa Mtb galur Malang dapat menginduksi respons imun seluler melalui sintesis IL-2 dan IFN- Ɣ limfositT. Metode Penelitian . Penelitian eksperimental invitro dengan subyek sehat endemik, kontak TB, penderita TB. PBMC dari darah subyek dikultur masing-masing dengan tiga perlakuan, tidak dipapar protein apapun, dipapar protein rekombinan 38 kDa Mtb galur Malang, dan dipapar PPD. Sintesis IL-2 dan IFN Ɣ intraseluler limfosit TCD3+ diperiksa menggunakan flowcytometry. Data dianalisis dengan uji Anova atau Kruskal Wallis. Hasil . Prosentase tertinggi IL-2 terdapat pada paparan protein rekombinan 38 kDa Mtb galur Malang, pada sehat endemik (p=0,000) dan kontak TB (p=0,000). Prosentase tertinggi IFN- Ɣ terdapat pada paparan protein rekombinan 38 kDa Mtb galur Malang , pada sehat endemik (p=0,007) dan kontak TB (p=0,105). Kesimpulan . Protein rekombinan 38 kDa Mtb galur Malang dapat meginduksi sintesis IL-2 dan IFN- Ɣ limfosit TCD3+ subyek sehat endemik dan kontak TB.
English Abstract
Background. Tuberculosis (TB) caused by Mycobacterium tuberculosis (Mtb), one of significant mortality causes. WHO (2012), placed Indonesia as great four of TB high burden countries. Primary immune response in TB pathogenesis is Cell mediated immunity (CMI) roled by T lymphocytes. Interleukin-2 (IL-2) is growth factor for T lymphocytes. Gamma Interferon is key cytokine in Mtb infection control, syn zised by T lymphocytes. Effective vaccination strategy achieved by giving vaccine able to stimulate T lymphocytes in syn zising cytokines. 38 kDa Mtb protein was potential in vaccine development program because its specific epitopes for T lymphocytes. Aim. To determine that 38 kDa recombinant protein of Mtb Malang strain able to induce cellular immune response by IL-2 and IFN- Ɣ syn zised by T lymphocytes. Methods. This was an invitro experimental study with healthy endemic, TB contact, and TB patient subjects. PBMC from subjects whole blood was cultured with 38 kDa recombinant protein of Mtb Malang strain, with PPD, and without any of Mtb protein. Analysis of IL-2 and IFN- Ɣ intracellular syn zised in T lymphocytes using flowcytometry. Data were analyzed by Anova or Kruskal Wallis test. Result. Highest percentage of IL-2 found in cultured with 38 kDa recombinant protein of Mtb Malang strain, in healthy endemic (p=0,000) and contact TB (p=0,000). Highest percentage of IFN- Ɣ found in cultured with 38 kDa recombinant protein of Mtb Malang strain, in healthy endemic (p=0,007) and TB contact (p = 0,105). Conclusion . 38 kDa recombinant protein of Mtb Malang strain able to induce IL-2 and IFN- Ɣ syn zised by TCD3+ lymphocytes from healthy endemic and TB contact.
| Item Type: | Thesis (Magister) |
|---|---|
| Identification Number: | TES/616.929 4/PRI/u/041403114 |
| Subjects: | 600 Technology (Applied sciences) > 616 Diseases > 616.9 Other disease |
| Divisions: | Profesi Kedokteran > Spesialis Patologi Klinik, Fakultas Kedokteran |
| Depositing User: | Endro Setyobudi |
| Date Deposited: | 23 Jun 2014 09:51 |
| Last Modified: | 23 Jun 2014 09:51 |
| URI: | http://repository.ub.ac.id/id/eprint/158453 |
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