Rusmini, Hetti (2013) Efek Pemberian Calcitriol [1,25(OH)2D3] Terhadap Diferensiasi Dan Produksi Interleukin-12p70 Pada Sel Dendritik Pasien Lupus Eritematosus Sistemik. Magister thesis, Universitas Brawijaya.
Abstract
Latar Belakang: Calcitriol [1,25(OH)2D3] memiliki kemampuan dalam mengontrol penyakit autoimun karena mempunyai efek terhadap regulasi respon imun, diantaranya menurunkan aktivasi sel dendritik. Penelitian ini bertujuan untuk mengetahui efek pemberian vitamin D terhadap diferensiasi dan produksi IL-12p70 pada sel dendritik pasien LES dengan hipovitamin D. Metode: Rancangan penelitian bersifat Eksperimental Design dengan menggunakan post test only group design. Sampel berupa sel monosit yang berasal dari 5 orang pasien LES Hipovitamin D. Kelompok penelitian dibagi menjadi 4 kelompok, yaitu P0 (tanpa 1,25(OH)2D3) sebagai kelompok kontrol dan P1 (1,25(OH)2D3 dosis 1 nM), P2 (1,25(OH)2D3 dosis 10 nM), P3 (1,25(OH)2D3 dosis 100 nM) sebagai kelompok perlakuan. Dilakukan analisa surface activation markers CD40, CD86, dan HLA-DR untuk menilai diferensiasi sel dendritik serta pengukuran kadar IL-12p70 menggunakan metode ELISA untuk menilai produksi sitokin IL-12p70. Hasil: Penelitian ini menunjukkan bahwa terdapat perbedaan yang tidak bermakna (p=0.051) antara ekspresi CD40 pada dosis kontrol (42.43±6.32%) dibandingkan dosis 1 nM (36.87±5.60%), 10 nM (31.66±6.43%), dan 100 nM (38.77±9.17%). Terdapat perbedaan bermakna (p=0,018) ekspresi CD86 pada kelompok dosis 10 nM (29.93±7.28%) terhadap kontrol (53.52±13.40%). Terdapat perbedaan yang tidak bermakna (p=0.550) pada antara ekspresi HLA-DR pada dosis kontrol (39.53±17.74%) dibandingkan dengan dosis 1 nM (34.74±17.45%), 10 nM (27.69±14.77%), dan 100 nM (31.97±17.08%). Terdapat perbedaan yang tidak bermakna (p=0.081) antara kadar IL-12p70 pada dosis kontrol (57.14±16.90%) dibandingkan dengan dosis 1 nM (44.10+25.13%), 10 nM (28.86±6.77%), dan 100 nM (48.52±15.92%). Terdapat korelasi positif yang bermakna antara ekspresi CD86 terhadap kadar IL-12p70 (p=0.015; r=0.536). Kesimpulan: Calcitriol [1,25(OH)2D3] merupakan metabolit aktif vitamin D yang dapat berperan sebagai imunomodulator pada sel dendritik pasien LES dengan cara menurunkan ekspresi CD86.
English Abstract
Backgorund: Calcitriol [1,25(OH)2D3] known has a ability to control autoimmune diseases because it has an effect on regulation of immune responses, including decrease activation of dendritic cells. This study aimed to determine effect of vitamin D in dendritic cells differentiation and production of IL-12 of systemic lupus ery matosus patients with hipovitamin D. Methods: experimental study design using post-test only group design. Monocytes was collected from five SLE patients with hipovitamin D. study group was divided into 4 groups, P0 (without 1,25(OH)2D3) as a control group and P1 (1,25(OH)2D3 dose 1 nM), P2 (1,25(OH)2D3 dose 10 nM), P3 (1,25(OH)2D3 dose 100 nM) as a treatments group. Analysis of surface activation markers CD40, CD86, and HLA-DR to assess differentiation of dendritic cells and measured levels of IL-12p70 using ELISA method to assess production of cytokine IL-12p70. Result: results showed that re were no significant differences (p=0.051) in CD40 expression between P0 compared with dose 1 nM, 10 nM, and 100 nM.. re were significant differences (p=0,018) in CD86 expression between 10 nM compared with control. re were no significant differences (p=0.550) in HLA-DR expression between control compared with 1nM, 10 nM, and 100 nM. re were no significant differences (p=0.081) in IL-12p70 level between control compared with 1 nM, 10 nM, and 100 nM. re were significant positive correlation between expression of CD86 and IL-12p70 levels (p=0.015; r=0.536). Conclusion: Calcitriol [1,25(OH)2D3] is an active metabolite of vitamin D that can play role as imunnomodulator on dendritic cells SLE patients by decreasing expression of CD86.
| Item Type: | Thesis (Magister) |
|---|---|
| Identification Number: | TES/616.772/RUS/e/041400682 |
| Subjects: | 600 Technology (Applied sciences) > 616 Diseases > 616.7 Diseases of musculoskeletal system |
| Divisions: | S2/S3 > Magister Ilmu Biomedis, Fakultas Kedokteran |
| Depositing User: | Budi Wahyono Wahyono |
| Date Deposited: | 13 May 2014 07:20 |
| Last Modified: | 13 May 2014 07:20 |
| URI: | http://repository.ub.ac.id/id/eprint/158414 |
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