Murdiyo, MohammadDwijo (2012) Hubungan Mutasi Onkogen H-Ras dan N-Ras Penderita Karsinoma Nasofaring WHO Tipe III Stadium Lanjut dengan Riwayat Merokok. Magister thesis, Universitas Brawijaya.
Abstract
Karsinoma nasofaring merupakan keganasan yang paling banyak dijumpai dibidang Telinga Hidung Tenggorok di Indonesia dan memiliki faktor penyebab multifaktor yang melibatkan peranan lingkungan, virus dan genetik. Salah satu faktor lingkungan yang berperan yaitu merokok. Rokok mengandung karsinogen utama yaitu PAH dan nitrosamin. Karsinogen ini dapat menyebabkan mutasi onkogen salah satunya gen ras dan deaktivasi gen supresor tumor. Penelitian ini untuk mengetahui hubungan mutasi gen ras penderita karsinoma nasofaring dengan riwayat merokok. Dengan metode studi cross sectional . Penelitian dilakukan pada 30 penderita karsinoma nasofaring WHO tipe III stadium lanjut. Mutasi gen diketahui dari pemeriksaan RFLP jaringan kanker nasofaring. Analisa statistik diuji dengan Fisher exact test dan uji t. Mutasi H-ras terdapat pada 1 dari 12 subyek dengan riwayat tanpa merokok, tidak mengalami mutasi pada 18 subyek dengan riwayat merokok. Mutasi N-ras terjadi pada 75% subyek, 10 dari 12 subyek tanpa riwayat merokok dan 11 dari 16 subyek dengan riwayat merokok. Hasil uji t mutasi H-ras terhadap jumlah rokok perhari (p=0.35), lama merokok (p=0.37), dan paparan kumulatif (p=0.47) menunjukkan tidak ada hubungan yang bermakna. Hasil uji t mutasi N-ras terhadap jumlah rokok perhari (p=0.23), lama merokok (p=0.46), dan paparan kumulatif (p=0.09) menunjukkan tidak ada hubungan yang bermakna. Kesimpulan: Mutasi N-ras lebih sering terjadi pada karsinoma nasofaring. Tidak ada hubungan yang bermakna antara mutasi H-ras dan N-ras terhadap riwayat merokok.
English Abstract
Nasopharyngeal carcinoma is most common carcinoma found in Ear-Nose-Throat department in Indonesia. It has multifactorial causes which involve environtmental, viral and genetic factor. One of environmental factor which has rule in nasopharyngeal carcinoma is smoking. Cigarette smoke consists of main carcinogens, PAH and nitrosamine. se carcinogens may cause mutation of oncogenes, such as ras gene and deactivation of tumor suppressor gene. Objective of this study is to know relation between mutation of ras gene in nasopharyngeal carcinoma patients and history of smoking. method is cross sectional. This study was performed in 30 patients with nasopharyngeal carcinoma WHO type III late stage. Gene mutation was examined with RFLP test of nasopharyngeal cancer tissues. Statistical analysis was performed with Fisher exact test and T test. Mutation of H-ras was found in 1 from 12 subjects without history of smoking. re was no mutation in 18 subjects with history of smoking. N-ras mutation was found in 75 percents subjects, 10 from 12 subjects without history of smoking and 11 from 16 subjects with history of smoking. T test result of H-ras mutation and sum of cigarettes per day (p=0.35), duration of smoking (p=0.37), and cumulative exposure (p=0,47) shows no significant relationship. T test result of N-ras mutation and sum of cigarettes per day (p=0.23), duration of smoking (p=0.46), and cumulative exposure (p=0,09) shows no significant relationship. Conclusion: N-ras mutation is more often happened in patients with nasopharyngeal carcinoma. re is no significant relationship between H-ras and N-ras mutation with history of smoking.
| Item Type: | Thesis (Magister) |
|---|---|
| Identification Number: | TES/616.21/MUR/h/041204695 |
| Subjects: | 600 Technology (Applied sciences) > 616 Diseases > 616.2 Diseases of respiratory system |
| Divisions: | Profesi Kedokteran > Spesialis THT Kepala dan Leher, Fakultas Kedokteran |
| Depositing User: | Endro Setyobudi |
| Date Deposited: | 26 Dec 2012 11:14 |
| Last Modified: | 26 Dec 2012 11:14 |
| URI: | http://repository.ub.ac.id/id/eprint/158307 |
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