Indahyanti, Fitri (2014) Protein Rekombinan 38 kDa Mycobacterium Tuberculosis Dapat Menginduksi Ekspresi IFN-γ Limfosit TCD3+ dan Direspon Imunoglobulin G Serum. Magister thesis, Universitas Brawijaya.
Abstract
Latar belakang: Tuberkulosis (TB) masih menjadi masalah kesehatan di seluruh dunia. Salah satu cara pengendalian TB dengan vaksinasi BCG yang sampai sekarang digunakan memberikan perlindungan terhadap penyakit TB yang berat pada anak, tetapi efektifitasnya pada dewasa bervariasi (20 %– 80%). Pada proses pengembangan vaksin, diperlukan uji imunogenitas untuk mengetahui antigenisitasnya. Tujuan penelitian ini untuk mengetahui respon antibodi (Ig G) serum dan prosentase sel limfosit TCD3+ - IFN- γ kultur PBMC setelah diinduksi protein rekombinan 38 kDa Mycobacterium tuberculosis (MTB). Metode: Terdapat 3 kelompok terdiri dari sehat, kontak TB dan penderita TB masing masing 8 orang. Respon Ig G serum diperiksa dengan metode dot blot sedangkan prosentase sel limfosit TCD3+-IFN- γ kultur PBMC dengan flowcitometry . Hasil: Ig G serum dapat mengenali protein rekombinan 38 kDa pada 3 kelompok dengan perbedaan tidak bermakna (p = 0,502). Pada induksi protein rekombinan 38 kDa MTB, prosentase sel limfosit TCD3+ - IFN- γ pada kelompok sehat (12.19±0,34) lebih tinggi secara bermakna (p=0,028) daripada kontak TB (8.81±0.34) dan penderita TB (6.39±2.72). Pada induksi protein rekombinan 38 kDa MTB, prosentase sel limfosit TCD3+- IFN- γ (12.19±0,34) lebih tinggi daripada induksi dengan PPD (7.43±0,18) atau tanpa perlakuan (11.18±0,38). Kesimpulan: Protein rekombinan 38 kDa MTB dapat dikenali Ig G serum dan menginduksi sel limfosit TCD3+-IFN- γ lebih tinggi pada kelompok sehat.
English Abstract
Backrground: Tuberculosis remains a worldwide health problem. One way in controlling TB is by performing BCG vaccination that provides protection against severe TB in children, but its effectiveness varies in adults (20-80%). In the process of developing a vaccine, immunogenicity test is required to determine the antigenicity. The purpose of the study is to determine antibody response (IgG) serum and TCD3+ lymphocyte IFN- γ percentage in PBMC cultures after 38kDa Mycobacterium tuberculosis (MTB) protein recombinant was induced. Method: There were 3 groups consist of healthy, TB contact, and TB patient groups with 8 persons in each group. IgG serum blood samples were obtained by using dot blot while TCD3+ lymphocyte IFN- γ percentage in PBMC cultures using flowcitometry. Result: IgG serum can recognize 38kDa protein recombinant in 3 groups with no significant difference (p=0,502). In the induction of recombinant protein 38 kDa MTB, the percentage of lymphocytes TCD3+- IFN- γ in the healthy group (12.19±0,34) was significantly higher (p = 0.028) than the TB contacts (8.81 ± 0.34) and TB patients (6.39±2.72). In the induction of recombinant protein 38 kDa MTB, the percentage of lymphocytes TCD3+- IFN- γ (12.19±0,34) was higher than induction with PPD (7.43±0,18) or without treatment (11.18±0,38). Conclusion : The 38 kDa MTB protein recombinant could be recognized by IgG serum and induced higher TCD3+lymphocyte-IFN- γ in healthy group.
| Item Type: | Thesis (Magister) |
|---|---|
| Identification Number: | TES/614.579/IND/p/041405843 |
| Subjects: | 600 Technology (Applied sciences) > 614 Forensic medicine; incidence of injuries, wounds, disease; public preventive medicine > 614.5 Incidence of and public measure to prevent specific disease and kinds of diseases |
| Divisions: | Profesi Kedokteran > Spesialis Pulmonologi dan Kedokteran Respirasi, Fakultas Kedokteran |
| Depositing User: | Endro Setyobudi |
| Date Deposited: | 05 Sep 2014 10:26 |
| Last Modified: | 05 Sep 2014 10:26 |
| URI: | http://repository.ub.ac.id/id/eprint/158000 |
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