Kurniawati, AdelyaDesi (2013) Effects of Fermentation on Compositional Changes of Cinnamomum osmophloeum Kaneh Leaves and Inhibitory Mechanism of Kaempferol on HepG2 Insulin Signaling Pathway. Magister thesis, Universitas Brawijaya.
Abstract
Cinnamomum osmophloeum Kaneh adalah salah satu spesies endemik di Taiwan, juga dikenal sebagai `adat kayu manis` karena konstituen daunnya mirip dengan minyak kayu manis yang terkenal. Sudah lama digunakan sebagai tanaman obat. Saat ini, orang-orang mengkonsumsi minuman fungsional seperti teh herbal untuk mengurangi perkembangan penyakit, seperti aterosklerosis, cedera inflamasi, kanker, penyakit kardiovaskular, dan juga diabetes. Fermentasi adalah salah satu proses produksi teh, yang dapat mengubah komposisi senyawa. Pengaruh fermentasi pada perubahan komposisi daun C. osmophloeum diselidiki. Metabolit sekunder bioaktif utama dalam C. ekstrak daun osmophloeum adalah dua flavonol glikosida, kaempferitrin dan kaempferol 3-o-β-d-glucopyranosyl- (1 → 4) -α-l-rhamnopyranosil-7-o-α-l-rhamnopyranoside. Dengan menggunakan HPLC dan LC-MS analisis komponen utama dan turunannya dikarakterisasi. Bakteri asam laktat diisolasi, dimurnikan, dan diidentifikasi dari daunnya yang fermentasi C. osmophloeum. Bakteri asam laktat utama diidentifikasi sebagai basil koagulan, lactobacillus plantarum, dan Piarococcus pentosaceus. Β-galactosidase yang dihasilkan oleh bakteri asam laktat pada daun fermentasi dihidrolisis flavonol glikosida ke aglikon, kaempferol. Efek penghambatan dari kaempferol pada jalur pensinyalan insulin dalam garis sel HEPG2 juga diselidiki. Hasil bloting barat menunjukkan bahwa kaempferol menonaktifkan fosforilasi P47 dan IRS1 (residu serine) dalam HEPG2 yang diobati dengan PMA melalui interaksi dengan protein PKC.
English Abstract
Cinnamomum osmophloeum Kaneh is one of the endemic species in Taiwan, also known as “indigenous cinnamon” because its leaves constituents are similar to the famous cinnamon oil. It has long been used as a medicinal plant. Nowadays, people consumed functional drinks such as herbs tea to reduce the progression of diseases, such as atherosclerosis, inflammatory injury, cancer, cardiovascular diseases, and also diabetes. Fermentation is one of the process on tea production, which could change the compounds composition. The effect of fermentation on the compositional changes of C. osmophloeum leaves was investigated. Main bioactive secondary metabolites in C. osmophloeum leaves extract are two flavonol glycosides, kaempferitrin and kaempferol 3-O-β-D-glucopyranosyl-(1 → 4)-α-L-rhamnopyranosyl-7-O-α-L-rhamnopyranoside. By using HPLC and LC-MS analysis the major components and its derivative were characterized. Lactic acid bacteria was isolated, purified, and identified from its fermented C. osmophloeum leaves. The major lactic acid bacteria are identified as Bacillus coagulans , Lactobacillus plantarum , and Pediococcus pentosaceus . The β-galactosidase produced by the lactic acid bacteria in the fermented leaves hydrolyzed the flavonol glycosides to aglycone, kaempferol. The inhibitory effect of kaempferol on insulin signaling pathway in HepG2 cell lines was also investigated. Western blotting results showed that kaempferol inactivated the phosphorylation of p47 and IRS1 (serine residues) in PMA-treated HepG2 through the interaction with PKC protein.
| Item Type: | Thesis (Magister) |
|---|---|
| Identification Number: | TES/583.23/KUR/e/041309011 |
| Subjects: | 500 Natural sciences and mathematics > 583 Magnoliopsida (Dicotyledons) > 583.2 Magnoliidae |
| Divisions: | S2/S3 > Magister Teknologi Hasil Pertanian, Fakultas Teknologi Pertanian |
| Depositing User: | Endro Setyobudi |
| Date Deposited: | 19 Feb 2014 11:28 |
| Last Modified: | 19 Feb 2014 11:28 |
| URI: | http://repository.ub.ac.id/id/eprint/157772 |
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