Destryana, RAmilia (2012) Biological Activities of Ocotea, Copaiba and Blue Cypress Essential Oils in Cultured Cells and in Mice. Magister thesis, Universitas Brawijaya.
Abstract
Minyak atsiri bersifat volatil, alami, kompleks yang ditandai dengan bau yang kuat dan dibentuk oleh tanaman aromatik sebagai metabolit sekunder. Mereka diperoleh dari buah-buahan, bunga, tunas, daun, biji, ranting, kulit batang, herbal, kayu dan akar banyak tanaman. Mereka telah banyak digunakan dalam pengobatan tradisional. Banyak studi penelitian dilakukan dengan beberapa minyak komersial memiliki komposisi kimia yang cukup berbeda dan pola farmakologis. Dalam penelitian ini, analisis GC dan GC / MS dari tiga minyak komersial minyak esensial seperti, Ocotea (ocotea quixos), copaiba (copaifera retikulata) dan minyak esensial copaifera cypress (callitris intratropica) dievaluasi. Minyak esensial Copaiba ditemukan mendominasi β-carylishlene (57,22%), γ -Eremene (12,87%) dan α -Humulene (6,52%) sebagai komponen utama. Sedangkan, minyak esensial OCOTEA ditandai dengan β-carylishlene (28,79%), metilkinamata (19,91%), β -selinene (10,58%), α -Humulene (10,35%), trans-cinnamaldehyde (1,04%) dan α - cosaene (0,07%). Di sisi lain, minyak esensial cypress biru ditemukan kandungan tinggi β-gurjunene (15,89%), α -eudesmol (15,29%), α -Patchoulene (13,36%) dan β -Amaaliene (11,31%). Minyak atsiri juga dievaluasi kegiatan antioksidan mereka menggunakan 2,2-diphenyl-1-picrylhydrazil (DPPH) dan uji pemutihan asam β-carotene-linoleat. 2 minyak esensial komersial hanya menunjukkan aktivitas antioksidan yang lemah, sedangkan Cypress biru menunjukkan aktivitas antioksidan moderat-tinggi di DPPH (58,62 ± 0,25%), dibandingkan dengan BHT 80 μg / mL (87,97 ± 0,26%). Minyak Cypress biru paling efektif untuk menghambat peroksidasi asam linoleat dengan persentase hambatan (69,00 ± 1,05%) dibandingkan dengan BHT 80 μg / ml (81,00 ± 0,71%). Hasil kami mengungkapkan bahwa tiga jenis minyak esensial komersial adalah penghambat manjur dari produksi mediator pro-inflamasi seperti nitric oxide (NO), prostagladin E2 (PGE2), interleukin-6 (IL-6), interleukin-1 β (IL -1 β), dan kemokin interleukin-8 (IL-8). Berdasarkan hasil ini, efek anti-inflamasi dari minyak esensial ini menunjukkan potensi aplikasi terapeutik dalam peraturan respons inflamasi.
English Abstract
Essential oils are volatile, natural, complex compounds characterized by a strong odour and are formed by aromatic plants as secondary metabolites. They obtained from fruits, flowers, buds, leaves, seeds, twigs, stem bark, herbs, wood and roots of many plants. They have been widely used in traditional medicine. Many of research studies were performed with some commercial oils have quite different chemical composition and pharmacological patterns. In this research, GC and GC/MS analysis of of three commercial oils essential oils such as, Ocotea ( Ocotea quixos ), Copaiba ( Copaifera reticulata ) and Blue Cypress ( Callitris intratropica ) essential oils were evaluated. The Copaiba essential oil was found to be predominated of β -caryophyllene (57.22%), γ –elemene (12.87%) and α -humulene (6.52%) as main components. Whereas, the essential oil of Ocotea was characterised by β -caryophyllene (28.79%), methylcinnamate (19.91%), β -selinene (10.58%), α -humulene (10.35%), trans-cinnamaldehyde (1.04%) and α -copaene (0.07%). On the other hand, the Blue Cypress essential oil was found high content of β -gurjunene (15.89%), α -eudesmol (15.29%), α -patchoulene (13.36%) and β -maaliene (11.31%). The essential oils were also evaluated of their antioxidant activities using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and β -carotene-linoleic acid bleaching tests. The 2 commercial essential oils showed only weak antioxidant activities, while Blue cypress showed a moderate-high antioxidant activities in DPPH (58.62 ± 0.25%), compared with BHT 80 μg/ml (87.97 ± 0.26%). Blue cypress oil was most effective for inhibiting linoleic acid peroxidation with inhibition percentage (69.00 ± 1.05%) compared with BHT 80 μg/ml (81.00 ± 0.71%). Our result revealed that three kind of commercial essential oils were potent inhibitors of the production of pro-inflammatory mediators such as nitric oxide (NO), prostagladin E2 (PGE2), interleukin-6 (IL-6), interleukin-1 β (IL-1 β ), and chemokines interleukin-8 (IL-8). Based on these result, the anti-inflammatory effects of these essential oils suggest potential therapeutic applications in the regulation of the inflammatory response.
| Item Type: | Thesis (Magister) |
|---|---|
| Identification Number: | TES/547.71/DES/b/041204161 |
| Subjects: | 500 Natural sciences and mathematics > 547 Organic chemistry > 547.7 Macromolecules and related compounds |
| Divisions: | S2/S3 > Magister Teknologi Hasil Pertanian, Fakultas Teknologi Pertanian |
| Depositing User: | Endro Setyobudi |
| Date Deposited: | 07 Apr 2014 09:48 |
| Last Modified: | 07 Apr 2014 09:48 |
| URI: | http://repository.ub.ac.id/id/eprint/157561 |
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