Arumdati, RaraAulia (2014) “Penentuan Mannitol Menggunakan Teknik PAD (Pulse Amperometry Detection) dan DPAD (Differential Pulsed Amperometry Detection)”. Sarjana thesis, Universitas Brawijaya.
Abstract
Mannitol yang bersifat elektroaktif dapat ditentukan secara amperometri. Analisis amperometri didasarkan pada arus difusi terhadap konsentrasi pada potensial dan waktu yang tetap. Oleh karena itu, penelitian ini melakukan optimasi potensial dan waktu penerapan potensial dengan teknik amperometri denyut, PAD (Pulsed Amperometry Detection) dan DPAD (Differential Pulsed Amperometry Detection) dalam sistem ruah dan sistem alir. Pada teknik PAD mannitol dioksidasi pada potensial kerja kemudian hasil oksidasi mannitol pada permukaan elektroda dibersihkan dengan potensial pembersih yaitu potensial oksidasi dan potensial reduksi. Dalam sistem ruah potensial kerja, potensial oksidasi dan potensial reduksi yang digunakan secara berturut-turut sebesar 0,3V selama 0,7 detik, 0,8V selama 0,6 detik, dan -0,8V selama 2,4 detik sedangkan dalam sistem alir sebesar 0,2V selama 0,7detik, 0,8V selama 0,6 detik, dan -0,8V selama 2,4 detik. Berbeda dengan PAD, DPAD menggunakan potensial background untuk mengetahui arus non faraday dalam pengukuran. Dalam sistem ruah digunakan potensial kerja, potensial background, potensial oksidasi, dan potensial reduksi secara berturut-turut sebesar 0,3V selama 0,7detik, -0,4V selama 0,7 detik 0,8V selama 0,6 detik, dan -0,8V selama 2,4 detik sedangkan dalam sistem alir sebesar 0,2V selama 0,7detik, - 0,3V selama 0,7 detik 0,8V selama 0,6 detik, dan -0,8V selama 2,4 detik. Kisaran konsentrasi linier, batas deteksi, sensitifitas, dan linearitas berturut-turut pada teknik PAD sistem ruah adalah 1x10-4 M sampai 1x10-3 M, 28 1M, 6396 1A/M, 0,992; pada teknik DPAD sistem ruah 1x10-4 M sampai 1x10-3 M, 20 1M, 5086 1A/M, 0,999; pada teknik PAD sistem alir 1x10-4 M sampai 1x10-2 M, 10 1M, 5040 1A/M, 0,999; pada teknik DPA
English Abstract
Mannitol is an electroactive compounds that can determined by amperometry. Amperometry analysis is based on the diffusion current to the concentration at constant potential and time. Therefore in this work, the potential and time were optimized by pulse amperometry, are PAD (Pulse Amperometry Detection) and DPAD (Differential Pulsed Amperometry Detection), in bulk and flow system. PAD technique, mannitol was oxidized by working potential then the reaction product was cleaned by cleaning potentials, are oxidation and reduction potentials. In bulk, the working potential, oxidation potential, and reduction potential, respectively, were 0.3V for 0.7s, 0.8V for 0.6s, and -0.8V for 2.4s whereas in flow system used 0.2V for 0.7s, 0.8V for 0.6s, and -0.8V for 2.4s. Differ from PAD, DPAD used background potential to know non farradaic curre t in measurement. In bulk system used working potential, background potential, oxidation potential, and reduction potential, respectively, were 0.3V for 0.7s, -0.4V for 0.7s, 0.8V for 0.6s, and -0.8V for 2.4s whereas in flow system used 0.2V for 0.7s, -0.3V for 0.7s, 0.8V for 0.6s, and -0.8V for 2.4s. Characteristic of measurement such as range of linear concentration, limit of detection, sensitivity, and linearity, respectively, by PAD in bulk system were 1x10-4 M to 1x 10-3 M, 28 1M, 6396 1A/M, 0.992; by DPAD in bulk system were 1x10-4 M to 1x10-3 M, 20 1M, 5086 1A/M, 0.999; by PAD in flow system were 1x10-4 M to 1x10-2 M, 10 1M, 5040 1A/M, 0.999; by DPAD technique in flow system were 1x10-4 M to 1x10-2 M, 63 1M, 5395 1A/M, 0.999.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/MIPA/2014/24/051400749 |
| Subjects: | 500 Natural sciences and mathematics > 540 Chemistry and allied sciences |
| Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam > Kimia |
| Depositing User: | Budi Wahyono Wahyono |
| Date Deposited: | 07 Feb 2014 15:40 |
| Last Modified: | 21 Oct 2021 04:21 |
| URI: | http://repository.ub.ac.id/id/eprint/153863 |
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