Santoso, Agus (2011) Potensi antioksidan dari monomer fenolik hasil degradasi lignin batang padi (Oryza sativa). Sarjana thesis, Universitas Brawijaya.
Abstract
Limbah batang padi di Indonesia masih memiliki daya guna yang rendah dengan jumlah limbah tiap tahun terus meningkat. Batang padi diketahui mengandung lignin dengan kadar 1,79%. Penelitian ini bertujuan untuk mengetahui monomer-monomer fenolik hasil degradasi lignin batang padi (Oryza sativa) dan mengetahui potensi senyawa hasil degradasi lignin sebagai antioksidan. Lignin batang padi dapat didegradasi menjadi monomer fenoliknya dengan menggunakan kombinasi reaktan H2O2-CuSO4-piridin. Komponen monomer lignin diidentifikasi dengan instrumen FT-IR dan Kromatografi Gas-Spektroskopi Massa (KG-SM). Aktivitas senyawa hasil degradasi lignin sebagai antioksidan diketahui berdasarkan nilai IC50 menggunakan metode DPPH (1,1 diphenyl-2-picrylhydrazyl). Hasil identifikasi monomer fenolik hasil degradasi lignin mengandung asam benzoat, p-hidroksi benzaldehida, p-hidroksi asetofenon, vanilin atau 4-hidroksi-3-metoksi benzaldehida dan 1,2 – asam benzendikarboksilat. Hasil uji aktivitas antioksidan menggunakan metode DPPH pada senyawa hasil degradasi lignin sebagai antioksidan dengan variasi konsentrasi (50, 150, 200, 250, 300, 350, 400, 450, 500 μg/mL) mempunyai nilai IC50 386,3 μg/mL.
English Abstract
Rice straw waste in Indonesia has low utility value with continuously increasing amount each year. Rice straw contains 1.79% lignin. This study was performed in order to determine the phenolic monomers of lignin degradation product of rice straw (Oryza sativa) and find out the potent of lignin degradation product compound as antioxidant. Rice straw lignin can be degraded into phenolic monomers by using reactants combination of H2O2-CuSO4-pyridine. Lignin monomer components were identified by FT-IR instrument and Gas Chromatography-Mass Spectroscopy (GC-MS). The activity of lignin degradation products as antioxidants was determined according to IC50 value by using DPPH (1,1-diphenyl-2-picrylhydrazyl) method. The identification result showed that lignin degradation product contained phenolic monomers of benzoic acid, p-hydroxy benzaldehyde, p-hydroxy acetophenone, vanillin or 4-hydroxy-3-methoxy benzaldehyde and 1,2-benzenedicarboxylic acid. The result of antioxidant activity test by using DPPH method toward lignin degradation compounds as antioxidant with various concentrations of (50, 150, 200, 250, 300, 350, 400, 450, 500 μg / mL) showed IC50 value of 386.3 μg/mL.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/MIPA/2011/258/051103365 |
| Subjects: | 500 Natural sciences and mathematics > 540 Chemistry and allied sciences |
| Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam > Kimia |
| Depositing User: | Unnamed user with email repository.ub@ub.ac.id |
| Date Deposited: | 02 Nov 2011 14:26 |
| Last Modified: | 22 Oct 2021 08:01 |
| URI: | http://repository.ub.ac.id/id/eprint/152703 |
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