Cahyono, Effendi Tri (2015) Proses Sakarifikasi dan Fermentasi Bioetanol Berbahan Baku Tandan Kosong Kelapa Sawit (Elais guineensis) Hasil Pretreatment Lindi Hitam. Sarjana thesis, Universitas Brawijaya.
Abstract
Bioetanol merupakan salah satu bahan bakar terbarukan yang kini banyak dikembangkan di Indonesia. Namun, prospek pengembangan bioetanol masih mengalami kendala karena masih menggunakan bahan baku berbasis pangan seperti singkong, jagung dan tetes tebu. Oleh karena itu untuk menghindari persaingan bahan baku pengadaan antara bahan pangan dan energy, bioetanol dialihkan kebiomassa, salah satunya yaitu tandan kosong kelapa sawit yang mempunyai kandungan selulosa sebesar 41,30–46,50%, bahkan di Indonesia kesediaan TKKS sangat melimpah berkisar 18,2 juta ton/tahun. Hal inilah yang menyebabkan tandan kosong kelapa sawit sangat prospek sebagai bahan baku bioetanol di Indonesia. Bioetanol lignoselulosa dibuat dari 3 tahapan yaitu pretreatment untuk mendegradasi lignin sehingga tidak menghambat proses sakarifikasi selulosa untuk memotong selulosa menjadi glukosa dan glukosa akan difermentasi menjadi alcohol. Metode penelitian ini menggunakan dua metode yaitu metode Separated Hydrolisis and Fermentation(SHF) dan metode Simultaneous Sacharification and Fermentation(SSF) dengan subtrat 15% yang sudah di pretreatment menggunakan larutan 100% lindihitam, 50% lindi hitam:50% NaOH, 30% lindi hitam:70% NaOH, dan 100% NaOH sebagai control. Proses sakarifikasi menggunakan enzim Ctex2 30 FPU dan Htex2 dengan perbandingan 1:5 dari enzim Ctex2. Kemudian proses fermentasi menggunakan yeast Saccharomyces cerevisiae 1% dari volume total 75 ml. kemudian dilanjutkan dengan penelitian menggunakan metode dan subtract terbaik dengan variasi enzim 20, 30, dan 40 FPU. Dari hasil penelitian didapatkan bahwa proses SSF dan subtrat 30% lindi hitam:70% NaOH merupakan metode dan subtrat terbaik yang menghasilkan kadar etanol 5.22 % (g/g) di 24 jam dengan control subtrat 100% NaOH sebesar 6.60% (g/g) kadar etanol. Sedangkan di jam sampel berikutnya mengalami penurunan kadar etanol. Sedangkan untuk variasi enzim didapatkan hasil terbaik pada 40 FPU yaitu dengan kadar etanol 5.37% (g/g) di 24 jam pertama. Kemudian di jam sampel berikutnya juga mengalami penurunan kadar etanol.
English Abstract
Bioethanol is a renewable fuel that is now being developed in Indonesia. However, prospects for the development of bioethanol still experiencing problems because it still uses food-based raw materials such as cassava, corn, and molasses. Therefore, to avoid raw material procurement competition between food and the energy, raw materials bioethanol diverted to biomass. One of them is oil palm empty fruit bunches, which has a cellulose content of 41.30% to 46.50%. In Indonesia available TKKS with abundant amount of approximately 18.2 million tons/year. This is why TKKS very suitable as raw material for bioethanol in Indonesia. Lignocellulosic bioethanol consists of three stages of the process: 1) pretreatment to degrade lignin so it does not interfere with the process of saccharification; 2) cellulose to cuts the cellulose into glucose and; 3) glucose is fermented into alcohol. This research method using two methods: 1) Separated Hydrolisis and Fermentation (SHF) method and 2) Simultaneous Sacharification and Fermentation (SSF) method with substrate 15% already in pretreatment using a solution of 100% black liquor; 50% of black liquor:50% NaOH; 30% black liquor:70% NaOH; and 100% NaOH as a control. Saccharification process using enzymes Ctex2 30 FPU and Htex2 with ratio 1:5 of the enzyme Ctex2. Then the fermentation process using the yeast Saccharomyces cerevisiae 1% of the total volume of 75 ml. Then research methods and best subtract with variation of the enzyme 20, 30, and 40 FPU. The result showed that the SSF and the substrate 30% of black liquor:70% NaOH is a method and best substrate which is produce ethanol 5:22% (g/g) in 24 hours with a control ix substrate 100% NaOH amounted to 6.60% (g/g) ethanol content. While the next sample decreased levels of ethanol.And for enzyme variations is obtained the best results at 40 FPU with the ethanol content 5:37% (g/g) in the first 24 hours.While the next sample also decreased levels of ethanol
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FTP/2015/283/051508572 |
| Uncontrolled Keywords: | TKKS, Pretreatmen(LindiHitam:NaOH), SHF, SSF, Enzim, Yeast, Etanol.,-Enzymes, Ethanol, Pretreatment (Black Liquor:NaOH), SHF, SSF, TKKS, Yeast. |
| Subjects: | 300 Social sciences > 338 Production > 338.1 Agriculture |
| Divisions: | Fakultas Teknologi Pertanian > Keteknikan Pertanian |
| Depositing User: | Budi Wahyono Wahyono |
| Date Deposited: | 21 Dec 2015 13:19 |
| Last Modified: | 22 Nov 2021 05:06 |
| URI: | http://repository.ub.ac.id/id/eprint/150183 |
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