Efek Pemberian Tepung Cangkang Udang Vannamei (Litopenaeus vannamei) Pada Tikus Putih (Rattus norvegicus) Model Ovariektomi Berdasarkan Kadar Kalsium Dalam Darah Dan Ekspresi TNF-alfa Tulang Femur

Khowatimi, Khusnul (2017) Efek Pemberian Tepung Cangkang Udang Vannamei (Litopenaeus vannamei) Pada Tikus Putih (Rattus norvegicus) Model Ovariektomi Berdasarkan Kadar Kalsium Dalam Darah Dan Ekspresi TNF-alfa Tulang Femur. Sarjana thesis, Universitas Brawijaya.

Abstract

Ovariektomi adalah proses sterilisasi pada hewan betina yang dilakukan dengan cara mengambil bagian ovarium. Pada masa menopause atau paska ovariektomi, ovarium berhenti memproduksi estrogen sehingga terjadi penurunan estrogen dan memicu terjadinya osteoporosis. Pada kondisi tersebut kadar kalsium dalam darah akan menurun dan terjadi peningkatan osteoklas untuk meresorpsi kalsium tulang serta terjadi ekspresi mediator inflamasi TNF-alfasehingga kepadatantulang menurun. Pencegahan osteoporosis paska ovariektomi dapat dilakukan dengan memberikan asupan kalsium yang diperoleh dari tepung cangkang udang vannamei (Litopenaeus vannamei).Tujuan penelitian ini yaitu untuk mengetahui pengaruh pemberian tepung cangkang udang vannamei (Litopenaeus vannamei) terhadap kadar kalsium dalam darah dan ekspresi TNF-alfa pada tikus putih model ovariektomi. Tikusterbagidalam 5 kelompok perlakuan yaitu kelompok (K-) tikus kontrol negatif, kelompok (K+) tikus kontrol positif, kelompok (P1) tikus ovariektomi dengan terapi dosis 500 mg/kgBB,kelompok (P2) tikus ovariektomi dengan terapi dosis 1000 mg/kgBB dan kelompok (P3)tikus ovariektomi dengan terapi dosis 1500 mg/kgBB. Pengukuran kadar kalsium darah dilakukan dengan uji kalsium darah dan TNF-alfa dilakukan dengan uji Immunohistokimia(IHK). Analisa data dilakukan secara kuantitatif menggunakan analisis ragam ANOVA dan uji lanjutan Tukey = 0,05%.Hasil penelitian menunjukkan bahwa pemberian tepung cangkang udang vannamei dengan dosis 1000 mg/kgBB efektif untuk pencegahan osteoporosis paska ovariektomiyang diikuti dengan peningkatan kadar kalsium dalam darah dan penurunan sitokin TNF-alfa secara signifikan (p < 0,05).

English Abstract

Ovariectomy is a process of female animals sterilizationthat is done by taking part of the ovary. When the female animals is menopause or after ovariectomy, the ovaries stop producing estrogen, so it causes a decrease of estrogen and leads to osteoporosis. In these conditions the calcium levels in the blood will decreases, and it causes a rise of osteoclast to resorp bone calcium and expression of inflammatory mediator TNF-alpha ismore expressed,then the bone density will decreases. Prevention of osteoporosis post ovariectomy can be done by providing calcium intake derived from vannamei shrimpshell flour. The purpose of this study is to determine the effect of vannamei shrimp shell flour against the calcium levels in the blood and the expression of TNF-alpha in the mice models of ovariectomy. Mice are divided into 5 groups: group (K) mice negative control, group (K +) mice positive control, group (P1) ovariectomized mice with dose 500 mg / kg, group (P2) ovariectomized mice with dose 1000 mg / kg and groups (P3) ovariectomized mice with dose 1500 mg / kg. Measurement of blood calcium levels was performed using blood calcium testing and TNF-alpha was performed with immunohistochemistry test. The result were analyzed qualitatively and quantitatively by analysis of variance ANOVA and advanced Tukey test = 0.05%.The results showed that the effect of vannamei shrimp shells flour at dose 1000 mg / kg is effective to prevent osteoporosis after ovariectomy that followed by increased of calcium levels in blood and decreased levels of cytokine TNF alpha significantly (p <0.05).

Item Type: Thesis (Sarjana)
Identification Number: SKR/FKH/2017/101/051706284
Uncontrolled Keywords: Ovariektomi, Osteoporosis, Tepung cangkang udang vannamei, Kadar kalsium darah, TNF-alfa
Subjects: 600 Technology (Applied sciences) > 616 Diseases > 616.7 Diseases of musculoskeletal system > 616.71 Diseases of bones
Divisions: Fakultas Kedokteran Hewan > Kedokteran Hewan
Depositing User: Yusuf Dwi N.
Date Deposited: 22 Aug 2017 03:22
Last Modified: 12 Dec 2020 11:36
URI: http://repository.ub.ac.id/id/eprint/1491
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