Aini, HerlinQurrotul (2011) Uji Aktivitas Antibakteri Penicillium Sp. Isolat Dari Daun Mangrove Sonneratia Caseolaris Dan Penicillium Notatum (Atcc 28089) Terhadap Staphylococcus Aureus Dan Escherichia Coli. Sarjana thesis, Universitas Brawijaya.
Abstract
Dalam penelitian ini menggunakan isolat Penicillium sp. Isolat dari daun mangrove S onneratia caseolaris hasil penelitian dari Firdaus et al., (2010) dan Penicillium notatum (ATCC 28089) koleksi laboratorium Fakultas kedokteran Universitas Brawijaya, Malang yang telah ditumbuhkan pada media PDA (Potatoes Dekstrose Agar). Prosedur penelitian ini melalui beberapa tahapan antara lain: Persiapan sampel, Proses isolasi P. notatum , Proses fermentasi, Proses ekstraksi dan Uji aktivitas antibakteri serta uji SDS-PAGE. Penelitian dilaksanakan di Laboratorium Mikrobiologi Fakultas Perikanan dan Ilmu Kelautan Universitas Brawijaya Malang, Laboratorium Mikrobiologi Fakultas Kedokteran Universitas Brawijaya Malang dan Laboratorium Biomedik Fakultas Kedokteran Universitas Brawijaya Malang yang dilaksanakan pada bulan Januari-Juni 2011. Pelaksanaan penelitian ini menggunakan metode deskriptif untuk mencapai tujuan yang utama yaitu untuk mengetahui aktivitas antibakteri Penicillium sp. isolat dari daun mangrove Sonneratia caseolaris dan Isolat Penicillium notatum (ATCC 28089) terhadap S. aureus dan E. coli . Pelaksanaan penelitian ini menggunakan metode deskriptif. Metode deskriptif yaitu metode yang menggambarkan suatu keadaan atau kejadian. Dalam penggunaan metode ini memusatkan perhatian pada pemecahan masalah yang ada sekarang dengan jalan mengumpulkan data, menyusun, menganalisa dan menginterpretasikannya.Tujuan utama penelitian ini untuk mengetahui aktivitas antibakteri Penicillium sp. Isolat dari daun mangrove Sonneratia caseolaris dan Isolat murni Penicillium notatum (ATCC 28089). Hasil penelitian diketahui bahwa untuk pengujian cakram. Pada ekstrak Penicillium sp. didapatkan hasil ekstrak terbaik yaitu memiliki diameter zona hambat sebesar 11,5 mm, dalam menghambat pertumbuhan bakteri Staphylococcus aureus dan Easherichia coli . Hasil terbaik tersebut digunakan untuk analisa senyawa bioaktif yang berperan dalam penghambatan melalui uji SDS-Page, terdapat 21 BM (Berat molekul) senyawa protein yang teredeteksi terdapat dalam sampel ekstrak miselia sp. Isolat dari daun mangrove Sonneratia caseolaris yang diduga sebagai senyawa antibakteri. Senyawa-senyawa bioaktif yang diduga terdapat pada Penicillium sp. Isolat dari daun mangrove Sonneratia caseolaris dengan berat molekul tersebut adalah 96,9852 kDa ; 88,75045 kDa ; 86,80342 kDa ; 79,43318 kDa ; 72,68872 kDa ; 66,51692 kDa ; 58,22772kDa ; 53,28376 kDa ; 50,9715kDa ; 46,64365kDa ; 43,64066 kDa ; 39,05914 kDa ; 35,74274kDa ; 32,70792 kDa ; 29,27415 kDa ; 26,20087 kDa ; 22,93577 kDa ; 20,07756 kDa ; 17,18996 kDa ; 14,71766 kDa ; 9,44418 kDa.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FPR/2011/86/051104776 |
| Subjects: | 300 Social sciences > 333 Economics of land and energy > 333.9 Other natural resources > 333.91 Water and lands adjoining bodies of water |
| Divisions: | Fakultas Perikanan dan Ilmu Kelautan > Manajemen Sumberdaya Perairan |
| Depositing User: | Endang Susworini |
| Date Deposited: | 17 Apr 2012 14:29 |
| Last Modified: | 21 Oct 2021 08:18 |
| URI: | http://repository.ub.ac.id/id/eprint/132918 |
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