Pengaruh Perlakuan Beberapa Agens Hayati terhadap Kelimpahan Bakteri Rizosfer yang Bersifat Antagonis bagi Bakteri R.solanacearum pada Tanaman Kentang Dataran Menengah

Aulia, Desi (2016) Pengaruh Perlakuan Beberapa Agens Hayati terhadap Kelimpahan Bakteri Rizosfer yang Bersifat Antagonis bagi Bakteri R.solanacearum pada Tanaman Kentang Dataran Menengah. Sarjana thesis, Universitas Brawijaya.

Abstract

Produksi kentang nasional Indonesia pada tahun tahun 2014 tercatat 1.347.815 ton dengan luas panen 66.531 ha (BPS, 2015). Propinsi Jawa Timur pada tahun 2014 merupakan salah satu pusat produksi kentang di Indonesia dengan area pertanaman kentang seluas 11.888 ha dengan produksi mencapai 208.270 ton (BPS, 2015). Penyakit tanaman merupakan salah satu kendala dalam budidaya kentang di Indonesia. Penyakit utama di pertanaman kentang yaitu layu bakteri (Ralstonia solanacearum). Kehilangan hasil panen akibat penyakit layu bakteri mencapai 40%. Patogen R.solanacearum bersifat tular-tanah, sehingga kontaminasinya mempengaruhi kesuburan tanah. Penelitian dilakukan selama 6 bulan dari Bulan November 2015 – Mei 2016 di lahan pertanian Dusun Dadapan, Desa Pandanrejo, Kecamatan Bumiaji Kota Batu dengan ketinggian tempat 800 m dpl untuk pengabilan sampel tanah bahan uji. Sedangkan isolasi dan identifikasi bakteri rizosfer dilakukan di Laboratorium Bakteriologi Jurusan Hama dan Penyakit Tumbuhan, Fakultas Pertanian, Universitas Brawijaya Malang. Rancangan penelitian menggunakan RAK dengan 6 perlakuan sebelum aplikasi dan 6 perlakuan setelah aplikasi dengan 3 kali ulangan. Perlakuan yang digunakan yakni aplikasi mikroba B. subtillis, Mikoriza, Trichoderma sp., P. fluorescens dan mikroba konsorsium. Variabel pengamatan meliputi kelimpahan dan keragaman bakteri rizosfer. Data hasil pengamatan dianalisis menggunkan analisis sidik ragam dan dilanjutkan dengan uji lanjutan DMRT dengan taraf 5%. Hasil penelitian menujukan bahwa kelimpahan bakteri rizosfer dipengaruhi oleh adanya aplikasi agens hayati. Kelimpahan bakteri rizosfer terseleksi menjadi 2 jenis bakteri yaitu bakteri antagonis dan bakteri R.solanacearum. Kelimpahan bakteri antagonis berpengaruh tidak signifikan terhadap kelimpahan bakteri R. Solanacerum. Kelimpahan bakteri antagonis dan bakteri R. solanacerum memiliki hubungan keeratan. Hubungan keeratan yang tinggi diperoleh dari kelimpahan bakteri antagonis setelah aplikasi agens hayati dengan nilai koefisien korelasi sebesar (r) 0.6161. Bakteri antagonis hasil isolasi berasal dari golongan genus Corynebacterium sp., genus Erwinia sp., genus Pantoea sp., genus Xanthomonas sp .dan diduga genus Neisseria sp. Peningkatan jumlah bakteri antagonis pasca aplikasi Agens hayati terjadi pada ke empat genus yatitu genus Erwinia sp., genus Pantoea sp., genus Xanthomonas sp. dan diduga genus Neisseria sp. yang sebesar 100%.

English Abstract

Indonesian national potato production in 2014 recorded 1,347,815 tonnes with a harvested area of 66 531 ha (CBS, 2015). East Java Province in 2014 was one of the centers of potato production in Indonesia with area covering 11 888 ha of potatoes with production reaching 208 270 tonnes (BPS 2015). Plant diseases is one of the obstacles in potato cultivation in Indonesia. The main diseases in potato is bacterial wilt (Ralstonia solanacearum). Loss of crops due to bacterial wilt disease reached 40%. R.solanacearum be borne pathogens-ground, so that the contamination affecting soil fertility. The study was conducted during the six months from November 2015 - May 2016 in the hamlet Dadapan farmland, village Pandanrejo, Bumiaji Kota Batu with altitude of 800 m above sea level for soil sample collection test material. While the isolation and identification of bacteria in the rhizosphere do Bacteriology Laboratory of the Department of Plant Pests and Diseases, Faculty of Agriculture, University of Brawijaya. The study design used RAK with 6 treatments and 6 treatment before application after application with three replications. The treatments used ie applications microbes B. subtillis, Mycorrhizae, Trichoderma sp., P. fluorescens and microbial consortium. Variables include the observations of the abundance and diversity of rhizosphere bacteria. The data were analyzed using the analysis of variance and continued with advanced test DMRT with the level of 5%. Results of research addressing that rhizosphere bacterial abundance is affected by the application of biological agents. Rhizosphere bacterial abundance selected to be 2 types of bacteria are bacteria and bacterial antagonists R.solanacearum. Abundance of antagonistic bacteria not significant effect on the abundance of R.Solanacerum. Abundance of bacteria and bacterial antagonists R. solanacerum have a relationship of closeness. The relationship of closeness obtained from the high abundance of antagonistic bacteria after application of biological agents with a correlation coefficient of (r) 0.6161. Antagonistic bacteria isolation results come from the genus Corynebacterium sp., Genus Erwinia sp., Genus Pantoea sp., Genus Xanthomonas sp .and allegedly genus Neisseria sp. Increasing the number of antagonistic bacteria after application of biological agents occurred in all four yatitu genus genus Erwinia sp., Genus Pantoea sp., Genus Xanthomonas sp. and allegedly genus Neisseria sp. which amounted to 100%.

Item Type: Thesis (Sarjana)
Identification Number: SKR/FP/2016/568/051609295
Subjects: 600 Technology (Applied sciences) > 632 Plant injuries, diseases, pests
Divisions: Fakultas Pertanian > Hama dan Penyakit Tanaman
Depositing User: Sugiantoro
Date Deposited: 09 Nov 2016 14:24
Last Modified: 19 Oct 2021 13:37
URI: http://repository.ub.ac.id/id/eprint/131576
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