Distribusi Isolat Jamur Dari Rizosfir Cabai, Jeruk, Tomat Dan Uji Patogenisitas Terhadap Tenebrio Molitor (Coleoptera: Tenebrionidae)

WildaAlAluf (2016) Distribusi Isolat Jamur Dari Rizosfir Cabai, Jeruk, Tomat Dan Uji Patogenisitas Terhadap Tenebrio Molitor (Coleoptera: Tenebrionidae). Sarjana thesis, Universitas Brawijaya.

Abstract

Rizosfir berperan sebagai habitat yang menyediakan sumber air, udara, nutrisi untuk mendukung kehidupan mikroorganisme tanah serta jamur patogen serangga. Isolat dari rizosfir jeruk mampu menghasilkan mortalitas tinggi serangga uji tungau Polyphagotarsonemus latus (Acari: Tarsonemidae) hingga 100% (Rohman, 2015). Mortalitas P. latus hingga 100% oleh isolat virulen dari rizosfir jeruk menunjukkan bahwa rizosfir berperan sebagai habitat jamur patogen serangga. Jamur pada tanah di hutan alami (natural forests) tidak hanya patogen tetapi juga oportunis dan koloniser sekunder (Sun dan Liu, 2008). Sementara penelitian Anggarini (2015), Arisona (2015) dan Rohman (2015) 14 isolat jamur dari rizosfir cabai, jeruk dan tomat semuanya disebut sebagai patogen serangga. Penelitian distribusi jamur pernah dilakukan pada tanah di hutan alami (natural forests) (Sun dan Liu, 2008) dan rizosfir tetapi pada berbagai tanaman sayuran (tomat, kubis bunga, sawi dan wortel) (Trizelia et al., 2015) sedangkan pada tanaman cabai, jeruk dan tomat belum diketahui. Penelitian ini dilaksanakan di sub Laboratorium Nematologi dan Virologi, Jurusan Hama dan Penyakit Tumbuhan, Fakultas Pertanian, Universitas Brawijaya, Malang mulai bulan Februari sampai September 2015. Rancangan penelitian pada uji patogenisitas adalah Rancangan Acak Lengkap (RAL) dengan menggunakan teknik inokulasi celup 14 isolat ke dalam suspensi 107 konidia/ml selama 5 (lima) detik dengan 3 (tiga) kali ulangan. Setiap isolat diinokulasikan 15 larva instar 10-11. Parameter pengamatan meliputi mortalitas larva Tenebrio molitor (Coleoptera: Tenebrionidae), waktu pengamatan dan larva T. molitor yang mati. Data mortalitas larva T. molitor dianalisis dengan menggunakan ANOVA, apabila hasil menunjukkan berbeda nyata, maka dilakukan uji Duncan pada taraf 5 %. Pengelompokan isolat jamur menjadi 3 (tiga) kelompok ditujukan untuk isolat jamur yang belum diketahui atau dilaporkan sebagai patogen serangga sebelumnya sehingga perlu diketahui asosiasinya berdasarkan mortalitas. Dari 14 isolat jamur terbagi 10 isolat oportunis dan 4 (empat) koloniser sekunder. Virulensi isolat jamur ditentukan oleh kerapatan dan viabilitas konidia jamur. Isolat jamur berturut-turut terdistribusi di rizosfir jeruk 8 (delapan) sedangkan di cabai 5 (lima) dan di tomat hanya 1 (satu) isolat. Dari 10 oportunis, terdistribusi di cabai 4 (empat) dan jeruk 6 (enam) isolat sedangkan 4 (empat) koloniser sekunder terdistribusi di cabai 1 (satu), jeruk 2 (dua) dan tomat 1 (satu) isolat.

English Abstract

The rhizosphere is a habitat that provides a source of water, air, nutrient and environment that supports the life of soil microorganisms and insect pathogenic fungi. Isolates from the rhizosphere of citrus capable of producing high mortality Polyphagotarsonemus latus (Acari: Tarsonemidae) mites of test insects up to 100% (Rohman, 2015). Mortality of P. latus up to 100% by virulent isolates from rhizosphere of citrus showed that rhizosphere serve as habitat for insect pathogenic fungi. Fungai on soil in natural forests not only pathogens but also opportunists and secondary colonizers (Sun and Liu, 2008). While research Anggarini (2015), Arisona (2015) and Rohman (2015) 14 fungal isolates from rhizosphere of chili, citrus and tomato are all referred to as insect pathogens. Research of distribution fungi have been done on the soil in natural forests (Sun and Liu, 2008) and rhizosphere but on a various vegetable crops (tomato, cauliflower, cabbage and carrot) (Trizelia et al., 2015) while in chili, citrus and tomato unknown. This research was conducted at the sub Laboratory of Nematology and Virology, Department of Pests and Plant Diseases, Faculty of Agriculture, University of Brawijaya, Malang from February to September 2015. Design of experiment to pathogenicity test was Completed Random Design using dipped inoculation technique 14 isolates into suspension of 107 conidia/ml during of 5 (five) seconds with 3 (three) repetition. This 15 larval instar 10-11 were inoculated for each isolates. The observed parameters include the mortality of T. molitor (Coleoptera: Tenebrionidae) larvae, observed of time and T. molitor larvae died. The data of T. molitor larvae mortality were analyzed using ANOVA, if the results showed the real influence then the Duncan test on level 5%. The fungal isolates grouped into 3 (three) groups is intended to isolate the fungus of unknown or already reported as insect pathogens, therefore the association based on mortality needs to be identified. From 14 fungal isolates divided 10 isolates as opportunists pathogens and four (4) isolates as secondary colonizers. Virulence of fungi is determined by the density and viability conidial fungi. Fungal isolates were distributed in rhizosphere of 8 (eight) citrus, 5 (five) chilis and 1 (one) tomato. From 10 opportunists, distributed in chili of 4 (four) and 6 (six) isolates in citrus whereas 4 (four) secondary colonizers distributed 1 (one) in chilli, 2 (two) in citrus and 1 (one) isolates in tomato.

Item Type: Thesis (Sarjana)
Identification Number: SKR/FP/2016/517/051609204
Subjects: 600 Technology (Applied sciences) > 632 Plant injuries, diseases, pests
Divisions: Fakultas Pertanian > Hama dan Penyakit Tanaman
Depositing User: Sugiantoro
Date Deposited: 19 Oct 2016 09:57
Last Modified: 19 Oct 2021 12:33
URI: http://repository.ub.ac.id/id/eprint/131521
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