Abrianto, Bagas (2018) Efek Pencegahan Arang Aktif Terhadap Kadar Low Density Lipoprotein (Ldl) Dan Gambaran Histopatologi Duodenum Pada Tikus Putih (Rattus Norvegicus) Model Hiperkolesterolemia. Sarjana thesis, Universitas Brawijaya.
Abstract
Hiperkolesterolemia adalah suatu kondisi dimana meningkatnya konsentrasi kolesterol dalam darah yang melebihi nilai normal.Arang aktif dapat menyerap ribuan kali beratnya sendiri zat-zat berupa gas, logam berat, racunracun dan bahan-bahan kimia lain, sehingga merupakan bahan yang efektif dan tidak berbahaya. Penelitian ini bersifat true experimental menggunakan Rancangan Acak Lengkap (RAL). Hewan model menggunakan tikus putih (Rattus norvegicus) strain Wistar jantan berumur 8 – 12 minggu, dengan berat badan antara 100-150 gr diadaptasikan selama 7 hari dan dibagi dalam enam kelompok perlakuanyaitu kelompok negatif, positif, kelompok perlakuan 1, 2, 3 dan kelompok perlakuan 4 (placebo). Pemberian diet hiperkolesterolemia menggunakan bahan asam kholat 0,1%, minyak babi 10%, dan kuning telur puyuh rebus segar 5 % dengan total pemberian 3,02 gr/2mLselama 14 hari. Suplementasi menggunakanarang aktif dengan dosis masing-masing 340 mg/ekor, 680 mg/ekor, 1020 mg/ekor dan placebo dengan dosis 680 mg/ekor tanpa pemberian diet hiperkolesterolemia. Pemberian dengan sonde lambung selama 14 hari. Pengukuran kadar Low Density Lipoprotein (LDL) menggunakan spektrofotometri, analisa data dilakukan secara kuantitatif menggunakan uji One Way ANOVA, α = 5% dan uji Tukey (p<0,01).Hasil gambaran histopatologi organ duodenum dianalisa secara deskriptif. Dari hasil yang didapat dapat ditarik kesimpulan bahwa pemberian arang aktif dapat mencegah hiperkolesterolemia dilihat dari pencegahan kerusakan histopatologi duodenum dan penghambatan kenaikan kadar LDL dengan dosis pemberian efektif 680 mg/ekor.
English Abstract
Hypercholesterolemia is a condition which the concentration of cholesterol in the blood increased more than it is normal value. Activated charcoal can absorb thousands times of its own weight gaseous substances, heavy metals, toxins and other chemicals substances, so that is an effective material and harmless one. This research has the quality of true experimental using Complete Random Design (RAL). This research uses male rats (Rattus norvegicus) Wistar strain aged 8-12 weeks, weighing between 100-150 grams. It is being adapted for 7 days and grouped into six different treatments. They are negative group, positive group, treatment groups 1, 2, 3 and the 4 (placebo) group. The giving hypercholesterolemic diet consists of cholic acid 0.1%, lard 10%, and fresh boiled quail egg yolks5% with the total of 3.02 gr/ 2 mL for 14 days. Supplementation using activated charcoal with each dose of 340 mg/150 gram BW, 680 mg/150 gram BW, 1020 mg/150 gram BW and the placebo at a dose of 680 mg/150 gram BW without giving any hypercholesterolemic diet with gastric sonde for 14 days. Low Density Lipoprotein (LDL) levels was measured using the spectrofotometry, quantitative data analysis was performed using One Way ANOVA, α = 5% and continued by the Tukey test (p <0.01). The results overview duodenum organ histopathology were analyzed descriptively. From the results obtained it can be conclude that activated charcoal giving can prevent hypercholesterlemia seen from prevention of duodenal histopathology damage and inhibition increase of LDL level with effective dose of 680 mg/150 gram BW.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FKH/2018/30/051801428 |
| Uncontrolled Keywords: | Hiperkolesterolemia, Arang aktif, Low Density Lipoprotein, Histopatologi Duodenum- Hypercholesterolemia, Activated charcoal, Low Density Lipoprotein,duodenum histopathology |
| Subjects: | 600 Technology (Applied sciences) > 616 Diseases > 616.3 Diseases of digestive system > 616.39 Nutritional and metabolic diseases > 616.399 Other nutritional and metabolic diseases / Malnutrition > 616.399 7 Diseases of lipid metabolism |
| Divisions: | Fakultas Kedokteran Hewan > Kedokteran Hewan |
| Depositing User: | soegeng sugeng |
| Date Deposited: | 08 Jan 2019 01:42 |
| Last Modified: | 21 Oct 2021 07:55 |
| URI: | http://repository.ub.ac.id/id/eprint/12799 |
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