Abdillah, Muhammad (2015) Analisis Perbedaan Gen Warna Rambut Lutung Jawa (Trachypithecus Auratus) Berdasarkan Sekuen Gen Melanocortin-1 Receptor (Mc1r) Dengan Metode Polymerase Chain Reaction (Pcr). Sarjana thesis, Universitas Brawijaya.
Abstract
Lutung Jawa (Trachypithecus auratus) merupakan primata endemik Pulau Jawa dan ditemukan individu dewasa yang memiliki warna rambut hitam dan oranye. Melanocortin- 1 Receptor (MC1R) merupakan gen penentu warna rambut dan kulit pada mamalia. Gen MC1R meregulasi melanosit untuk membentuk pigmen melanin yang kemudian pigmen tersebut menghasilkan eumelanin dan feomelanin. Individu yang memiliki eumelanin memiliki rambut berwarna hitam dibandingkan dengan individu yang memiliki feomelanin lebih memiliki rambut oranye hingga kemerahan. Penelitian ini bertujuan untuk menentukan perbedaan warna rambut Lutung Jawa berdasarkan sekuen gen MC1R dengan mengisolasi DNA pada folikel rambut lalu dilakukan uji kuantitas dan kualitas DNA, kemudian diamplifikasi menggunakan metode PCR dengan primer MC1R_F 5‘-TCT ATGCACTGCGCTACCAC-3’ dan MC1R_R 5‘-CGTACAGCACAGCCATGAGT-3’, dan dilanjutkan dengan sekuensing terhadap produk PCR serta analisis dilakukan dengan menggunakan software BioEdit dan NCBI BLAST. Hasil penelitian menunjukkan sampel O1 dan H2 memiliki perbedaan basa nukleotida database genebank MC1R pada NCBI. Perubahan Sampel O1 terdapat pada c.596G>A. Telah terjadi perubahan susunan kodon pembentuk asam amino p.166A>T pada daerah transmembrane region gen MC1R. Terjadinya perubahan asam amino p.166A>T (non-polar menjadi polar) diprediksi dapat menghambat sintesis eumelanin sehingga individu Lutung Jawa rambut oranye tetap mensintesis feomelanin. Pada sampel H2, c.560C>G terjadi perubahan susunan kodon asam amino p.187A>G (non-polar menjadi non-polar) pada daerah extracellular loops gen MC1R. Kesimpulan dari penelitian ini adalah terdapat perbedaan sekuen DNA dan asam amino antara Lutung Jawa rambut hitam dan oranye.
English Abstract
Javan langur is an endemic primate in Java Island. There are two types of Javan langur; the black hair color and the orange hair color with freckles and fair skin. MC1R is an important gene to determine hair and skin color in mammals. MC1R regulates the melanosit into melanin pigment to form melanin pigment produces eumelanin and pheomelanin. Individual with most eumelanin has brown or black hair and dark skin. On the other hand, the one with pheomelanin has orange-red hair with freckles and fair skin. This study was aimed to determine the differences of hair color Javan Langur based on sequence of MC1R gene. DNA was isolated inside the hair follicles and tested both qualitative and quantitative, and the DNA was amplified by PCR with primers MC1R_F of 5‘-TCTATGCACTGC GCTACCAC-3’ and MC1R_R of 5‘-CGTACAGCACAGCCATGAGT-3’. Furthermore, sequences of the PCR product were analyzed using NCBI BLAST and BioEdit software. The results showed that the samples O1 and H2 have different nucleotide bases compared to MC1R Gene Bank database at NCBI. Sample O1 altered in c.596G>A. An alteration has occurred in the composition of the codon-forming amino acid p.166A> T (nonpolar into polar) in the transmembrane region of MC1R. This alteration was predicted could inhibit synthesis of eumelanin so that the orange hair Javan Langur still synthesize pheomelanin. An alteration of nucleotide (c.560C>G) also occurred in sample H2 which affected the codon of amino acid p.187A> G (non-polar to non-polar) in the extracellular loops of MC1R. This study concluded there are differences of DNA sequences and amino acid between Javan Langur with black hair color and orange hair color.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FKH/2015/136/ 051602814 |
| Subjects: | 600 Technology (Applied sciences) > 636 Animal husbandry > 636.08 Specific topics in animal husbandry > 636.089 Veterinary medicine |
| Divisions: | Fakultas Kedokteran Hewan > Kedokteran Hewan |
| Depositing User: | Kustati |
| Date Deposited: | 23 May 2016 13:54 |
| Last Modified: | 26 Nov 2021 03:33 |
| URI: | http://repository.ub.ac.id/id/eprint/127003 |
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