Pradipto, SepthaRullyDwi (2017) Pengaruh Vaksin Hipertensi Berbahan Dasar Outer Membrane Protein Porphyromonas Gingivalis Terhadap Tekanan Darah Tikus Model Hipertensi. Sarjana thesis, Universitas Brawijaya.
Abstract
Hipertensi merupakan penyakit kronis yang paling banyak diderita di dunia yang terjadi karena peningkatan reactive oxygen species (ROS) yang berhubungan dengan disfungsi endotel. Peningkatan ROS menyebabkan peroksidasi lipid yang menghasilkan produk Malondialdehid (MDA). Outer membrane protein (OMP) Porphyromonas gingivalis, bakteri yang menyebabkan periodontitis, memiliki protein gingipain (Rgp) yang diketahui memiliki mimikri molekuler dengan MDA-LDL. Penelitian ini bertujuan untuk membuktikan pengaruh pemberian OMP bakteri P. gingivalis terhadap tekanan darah tikus model hipertensi. Penelitian ini adalah eksperimen murni di laboratorium secara in vivo dengan metode Randomized Post Test Only Controlled Group Design. Hewan coba 25 tikus wistar (Rattus norvegicus) jantan usia 7-8 minggu dibagi menjadi 5 kelompok yaitu kontrol positif, kontrol negatif, perlakuan 1 (OMP (100μl)), perlakuan 2 (OMP+CFA/IFA (200μl)), dan perlakuan 3 (CFA/IFA (100μl)). Perlakuan diawali dengan pemberian OMP P.gingivalis secara intraperitoneal dengan komposisi yang telah ditentukan untuk masing-masing kelompok. Setelah 2 minggu dari waktu pemberian OMP kemudian dilakukan pemberian garam DOCA secara subkutan dengan dosis 10 mg/kgBB sebanyak 2 kali dalam seminggu selama 5 minggu. Pemberian OMP sebagai vaksin ulangan diberikan sebanyak 3 kali dengan jarak 10 hari dari waktu pemberian dosis vaksin sebelumnya. Setelah pemberian vaksin dan garam DOCA, dilakukan pengukuran tekanan darah darah tikus. Hasil analisis statistik dengan uji one way ANOVA menunjukkan penurunan tekanan darah tikus (p=0,000). Dari hasil penelitian ini dapat disimpulkan bahwa pemberian OMP P. gingivalis dapat mencegah peningkatan tekanan darah pada tikus model hipertensi.
English Abstract
Hypertension is a chronic disease that most affects the world. This is influenced by the increase in Reactive Oxygen Species (ROS) associated with endothelial dysfunction. Increased ROS caused lipid peroxidation that produced malondialdehyde (MDA). Outer membrane protein (OMP) Porphyromonasgingivalis, bacteria causing periodontitis, has gingipain protein is known to have mimicry molecular with MDA-LDL. This study aimed to verify the effect of administration of OMP P.gingivalis to blood pressure in Hypertension rat models. This is a pure experimental research in the laboratory using in vivo methods with Post Test Only Randomized Controlled Group Design. Animals, 25 Wistar rats (Rattusnorvegicus) male 7-8 weeks of age, were divided into 5 groups: positive control, negative control, treatment 1 (OMP 100μl), treatment 2 (OMP+CFA/IFA 200μl), and treatment 3 (CFA/IFA 100μl). At the beginning of the study, three treatment groups of rats were injected OMP intraperitoneally in accordance with the provisions. After 2 weeks, DOCA salt was given subcutaneously ie 10 mg/kgBW twice a week for 5 weeks. Administration of OMP was repeated 3 times in every 10 days intraperitoneal after previous administration. Then the blood pressure of rats will be measured. The results of the analysis with the one way Anova showed decreased blood pressure (p=0.000). From these results it can be concluded that the administration of P. gingivalis’ OMP can prevent rising ofblood pressure in hypertension rat models.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FK/2016/804/051701988 |
| Subjects: | 600 Technology (Applied sciences) > 617 Surgery, regional medicine, dentistry, ophthalmology, otology, audiology > 617.6 Dentistry |
| Divisions: | Fakultas Kedokteran Gigi > Kedokteran Gigi |
| Depositing User: | Kustati |
| Date Deposited: | 22 Mar 2017 09:46 |
| Last Modified: | 19 Oct 2021 04:24 |
| URI: | http://repository.ub.ac.id/id/eprint/126520 |
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