Amilia, Etis (2016) Efek Ekstrak Etanol Daun Nilam (Pogostemon cablin folium) dalam Menghambat Pertumbuhan Candida albicans Secara In Vitro. Sarjana thesis, Universitas Brawijaya.
Abstract
Sekitar 55-75% dari semua wanita pernah mengalami kandidiasis vulvovagina selama masa hidupnya, dan 40-50% dari mereka mengalami infeksi berulang. Obat yang sering digunakan untuk kandidiasis vulvovagina adalah obat golongan azol. Penelitian ini bertujuan untuk membuktikan bahwa ekstrak etanol daun Nilam (Pogostemon calbin folium) memiliki efek antifungi terhadap Candida albicans secara in vitro. Daun nilam diekstraksi dengan menggunakan metode maserasi. Studi eksperimental dilakukan pada spesies Candida albicans menggunakan metode difusi lubang/sumuran. Konsentrasi ekstrak etanol daun nilam (25%, 37,5%, 50%, 62,5%, 75%, 87,5%, dan 100%) dimasukkan ke dalam masing-masing lubang dan diinkubasi selama 18-24 jam. Pengukuran diameter zona hambat yang terbentuk di sekitar lubang sumuran dilakukan dengan mengunakan jangka sorong. Dari hasil uji One way ANOVA pada data yang didapatkan dari penelitian diperoleh angka signifikansi 0,000 (p < 0,05). Hal ini menunjukkan bahwa efek perubahan konsentrasi ekstrak etanol daun nilam terhadap pertumbuhan Candida albicans adalah berbeda secara signifikan pada taraf kepercayaan 95%. hasil uji korelasi didapatkan nilai koefisien korelasi Pearson bernilai positif yaitu r = 0.985 yang menunjukkan bahwa semakin tinggi konsentrasi ekstrak etanol daun nilam maka semakin besar zona hambatnya. Dari uji regresi dapat diketahui R 2 sebesar 0,971 artinya bahwa 97,1% diameter zona hambatan pertumbuhan dipengaruhi oleh variabel bebasnya, yaitu konsentrasi ekstrak daun nilam. Kesimpulan dari penelitian ini adalah ekstrak etanol daun nilam memiliki efek antifungi terhadap Candida albicans dan semakin tinggi konsentrasi ekstrak etanol daun nilam maka semakin besar diameter zona hambatnya terhadap Candida albicans.
English Abstract
Approximately 55-75% of all women have experienced candidiasis vulvovagina during her lifetime, and 40-50% of those experiencing recurrent infections. Drugs are often used for candidiasis vulvovagina are azoles drug. This study aims to prove that the ethanol extract of leaves of Patchouli (Pogostemon calbin folium) has antifungal effect against Candida albicans in vitro. Patchouli leaves were extracted using maceration method. Experimental studies conducted on the species Candida albicans using diffusion holes. Concentration of ethanol extract of patchouli (25%, 37.5%, 50%, 62.5%, 75%, 87.5%, and 100%) is inserted into each hole and incubated for 18-24 hours. Measuring the diameter of inhibition zone formed around the hole wells is done by using calipers. From the test results One way ANOVA on data from research figures obtained significance of 0.000 (p <0.05). This suggests that the effect of changes in the concentration of ethanol extract of patchouli leaves to the growth of Candida albicans is significantly different at the 95% confidence level. Correlation test results obtained value of the Pearson correlation coefficient is positive, ie r = 0985 which showed that the higher concentration of ethanol extract of patchouli leaves has greater inhibitory zone. From the regression test can be known R of 0.971 means that the 97.1% growth inhibition zone diameters are influenced by independent variables, concentration of patchouli leaf extract. The conclusion of this study is the ethanol extract of patchouli leaf has antifungal effect against Candida albicans and the higher concentration of ethanol extract of patchouli leaves has greater diameter of inhibitory zone against Candida albicans.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FK/2016/755/051700319 |
| Subjects: | 600 Technology (Applied sciences) > 618 Gynecology, obstetrics, pediatrics, geriatrics > 618.2 Obstetrics |
| Divisions: | Fakultas Kedokteran > Kebidanan |
| Depositing User: | Sugiantoro |
| Date Deposited: | 13 Feb 2017 10:13 |
| Last Modified: | 19 Oct 2021 03:41 |
| URI: | http://repository.ub.ac.id/id/eprint/126465 |
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