Uji Potensi Anti-Proteasom Ubiquitin terhadap Peningkatan Densitas Protein Terpoliubiquitinasi pada Kultur Plasmodium falciparum

Anandi, Lazuardiah (2014) Uji Potensi Anti-Proteasom Ubiquitin terhadap Peningkatan Densitas Protein Terpoliubiquitinasi pada Kultur Plasmodium falciparum. Sarjana thesis, Universitas Brawijaya.

Abstract

Ubiquitin Proteasome System (UPS) adalah suatu system yang mengontrol kualitas protein pada siklus hidup Plasmodium falciparum. Sistem ini sangat berperan penting dalam regulasi siklus sel Plasmodium dengan cara mengkonjugasi seluruh bentuk dari protein untuk membentuk kompleks protein terpoliubiquitinasi yang pada akhirnya akan didegradasi oleh proteasom 20s. Penelitian ini merupakan penelitian eksperimental unutk mengetahui kemampuan dari antibodi poliklonal terhadap proteasome yang diproduksi dari kelinci dalam menghambat pertumbuhan dari P.falciparum in vitro. Kultur Plasmodium falciparum dibagi menjadi 1 Kelompok Kontrol Positif (Kultur P.falciparum tanpa perlakuan), 3 Kelompok Perlakuan (Kultur P.falciparum yang diberikan perlakuan antibodi poliklonal terhadap proteasome dengan dosis masing-masing 100µg/mL, 200µg/mL, 300µg), dan 1 Kelompok Plasebo (Kultur P.falciparum yang diberikan perlakuan Tris-HCL 200µg/mL dan adjuvan). Penumpukan protein terpoliubiquitinasi diukur menggunakan metode Western blot. Hasil Wester blot menunjukkan densitas kompleks protein terpoliubiquitinasi yang lebih tinggi pada kelompok yang diberikan perlakuan dibandingkan dengan kelompok control. Berdasarkan hal tersebut, dapat disimpulkan bahwa antibodi poliklonal terhadap proteasom potensial untuk dijadikan kandidat terapi/vaksin malaria baru melalui penghambatan UPS dan menyebabkan kematian Plasmodium.

English Abstract

Ubiquitin Proteasome System (UPS) is a system that control protein quality in Plasmodium falciparum life cycle. It is responsible for the Plasmodium’s cell cycle regulation by conjugating all stage of the protein to form the poly ubiquitin-protein complex which finally will be degraded by the 20s proteasome. This study was an experimental study to explore the capability of proteasome polyclonal antibody produced from Rabbit in inhibiting the growth of P.falciparum in vitro. Plasmodium falciparum cultures were divided into 1 positive control group (P.falciparum culture without any exposure), 3 treatment groups (P.falciparum culture exposed by proteasome polyclonal antibodies with dose of 100µg/mL, 200µg/mL, 300µg/mL respectively) and 1 placebo group (P.falciparum culture exposed by 200µg/mL Tris-HCL and adjuvant). The poly-ubiquitin protein accumulation was measured using Western blot method. Western blot result showed higher density of poly ubiquitin-protein complex in exposure groups than control group. It can be concluded that proteasome polyclonal antibody is potential for a new vaccine candidate and malarial medication by inhibiting the UPS and cause the Plasmodium’s death.

Item Type: Thesis (Sarjana)
Identification Number: SKR/FK/2015/192/051501635
Subjects: 600 Technology (Applied sciences) > 610 Medicine and health > 610.7 Education, research, nursing, services of allied health personnel
Divisions: Fakultas Kedokteran > Pendidikan Dokter
Depositing User: Budi Wahyono Wahyono
Date Deposited: 25 Feb 2015 07:32
Last Modified: 22 Oct 2021 07:10
URI: http://repository.ub.ac.id/id/eprint/124940
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