Fitriah, Athira (2014) Uji Daya Antihelmintik Ekstrak Etanol Kulit Buah Melinjo (Gnetum gnemon) Terhadap Cacing Ascaris suum Secara In vitro. Sarjana thesis, Universitas Brawijaya.
Abstract
Penyakit infeksi merupakan salah satu masalah utama di Indonesia. Salah satunya disebabkan oleh cacing jenis Askaris. Cacing Ascaris suum berasal dari genus yang sama seperti Ascaris lumbricoides, serta memungkinkan untuk dilakukan model terhadap penelitian Ascaris lumbricoides dengan cara in vitro. Tujuan dari penelitian ini yaitu untuk mengetahui adanya daya antihelmintik ekstrak etanol kulit buah melinjo (Gnetum gnemon) terhadap cacing Ascaris suum secara in vitro dan untuk mengetahui lethal concentration 100 (LC100) serta lethal time (LT100) dari ekstrak etanol kulit buah melinjo. Penelitian ini menggunakan eksperimen laboratorium untuk melihat daya antihelmintik ekstrak etanol kulit buah melinjo (Gnetum gnemon) dengan konsentrasi 30%, 40%, dan 50% dengan pembanding pirantel pamoat 1% sebagai kontrol positif dan FCS 1% dalam PBS sebagai kontrol negatif. Rancangan penelitian ini adalah post test only control group design, dengan subjek penelitian yaitu Ascaris suum yang didapat dari Rumah Pemotongan Hewan di Gadang. Data hasil penelitian dianalisis dengan analisa probit menggunakan aplikasi Minitab 17 untuk mengetahui LC100 dan LT100 dari ekstrak etanol kulit buah melinjo. Hasil penelitian menunjukkan bahwa LC100 pada ekstrak etanol kulit buah melinjo 50% sebesar 45,26% sedangkan LT100 ekstrak etanol kulit buah melinjo 50% adalah 9 jam 26 menit. Kesimpulan yang dapat diperoleh adalah ekstrak etanol kulit buah melinjo (Gnetum gnemon) memiliki daya antihelmintik terhadap cacing Ascaris suum, LC100 pada ekstrak etanol kulit buah melinjo sebesar 45,26%, dan LT100 ekstrak etanol kulit buah melinjo pada konsentrasi 50% adalah 9 jam 26 menit.
English Abstract
Infectious diseases are one of the major problems in Indonesia. One of them caused by a worm, Ascaris. Ascaris Suum derived from the same genus as Ascaris lumbricoides, and allows to be a model for the study of Ascaris lumbricoides by in vitro. The purpose of this study was to determine the presence of ethanol extract anthelmintics efficacy of rind melinjo (Gnetum gnemon) against Ascaris Suum in vitro and to determine the lethal concentration of 100 (LC100) and lethal time (LT100) of ethanol extract of the rind of melinjo. This study was using an laboratory experiments to see the efficacy of anthelmintics ethanol extract of the melinjo’s rind (Gnetum gnemon) with three concentrations of 30%, 40%, and 50% with comparator, a pyrantel pamoate 1% as a positive control and 1% FCS in PBS as a negative control. The design of this study was post test only control group design, with Ascaris Suum research subjects was obtained from slaughtering houses in Gadang. The data were analyzed by probit analysis using Minitab 17 application to determine LC100 and LT100 of ethanol extract of melinjo’s rind. The results showed that the ethanol extract LC100 rind melinjo 50% at 45.26% while the ethanol extract of the melinjo’s rind LT100 50% was 9 hours 26 minutes. The conclusion was the ethanol extract of the melinjo’s rind (Gnetum gnemon) has the anthelmintics effect against Ascaris Suum LC100 the ethanol extract of the rind melinjo by 45.26%, and LT100 at 50% concentration was 9 hours 26 minutes.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FK/2015/179/051501211 |
| Subjects: | 600 Technology (Applied sciences) > 610 Medicine and health > 610.7 Education, research, nursing, services of allied health personnel |
| Divisions: | Fakultas Kedokteran > Pendidikan Dokter |
| Depositing User: | Budi Wahyono Wahyono |
| Date Deposited: | 13 Feb 2015 13:45 |
| Last Modified: | 22 Oct 2021 06:55 |
| URI: | http://repository.ub.ac.id/id/eprint/124926 |
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