Uji Efek Antibakteri Ekstrak Etanol Daun Salam (Syzygium polyanthum) terhadap Streptococcus pyogenes secara In Vitro.

Dewanti, KesumaDIW (2014) Uji Efek Antibakteri Ekstrak Etanol Daun Salam (Syzygium polyanthum) terhadap Streptococcus pyogenes secara In Vitro. Sarjana thesis, Universitas Brawijaya.

Abstract

Infeksi endodontik adalah infeksi polimikroba pada pulpa dan saluran akar gigi yang terutama disebabkan oleh bakteri Streptococcus pyogenes. Daun salam (Syzygium polyanthum) memiliki kandungan yang bersifat sebagai zat antibakteri, antara lain, minyak atsiri, flavonoid, dan tanin. Penelitian ini bertujuan untuk mengetahui efek antibakteri ekstrak daun salam terhadap Streptococcus pyogenes secara in vitro. Desain penelitian yang digunakan adalah true experiment–post test only control group design dengan menggunakan metode dilusi agar dan dilusi tabung. Konsentrasi ekstrak daun salam yang digunakan adalah 0,5%, 1%, 1,5%, 2%, 2,5%, dan 3%. Data dianalisa secara statistik menggunakan uji one-way ANOVA, uji korelasi Pearson dan uji regresi dengan α = 0,05. Hasil penelitian menunjukkan bahwa ekstrak etanol daun salam mempunyai efek antibakteri terhadap pertumbuhan bakteri Streptococcus pyogenes dengan nilai KHM sebesar 2% dan KBM sebesar 2,5%, serta semakin tinggi konsentrasi ekstrak etanol daun salam maka semakin rendah pertumbuhan bakteri Streptococcus pyogenes. Kesimpulan berdasarkan penelitian ini adalah ekstrak etanol daun salam mempunyai efek antibakteri terhadap Streptococcus pyogenes secara in vitro. Berdasarkan hasil penelitian ini, perlu dilakukan penelitian untuk mengetahui zat antibakteri utama daun salam, serta penelitian lebih lanjut secara in vivo maupun terhadap manusia.

English Abstract

Endodontic infection is a polymicrobial infection of the pulp and root canals, mainly caused by Streptococcus pyogenes . Bay leaf ( Syzygium polyanthum ) have antibacterial agents contain essential oils , flavonoids , and tannins . This study aimed to determine the antibacterial effects of bay leaves extracts against Streptococcus pyogenes in vitro . The design research is a true experiment - post test only control group design using dilution agar method and dilution tube method. Concentration of bay leaves extract which used was 0,5 % , 1 % , 1,5 % , 2 % , 2,5 % , and 3 % . The data were statistically analyzed using one- way ANOVA test , Pearson correlation test and regression test with α = 0,05 . The results showed that the ethanol extract of bay leaves have antibacterial effect on the growth of Streptococcus pyogenes with MIC value is 2% and MBC value is 2,5%, as well as the higher concentration of ethanol extract of bay leaves , the lower the growth of the bacteria Streptococcus pyogenes . The conclusion of this study is ethanol extract of bay leaves have antibacterial effects against Streptococcus pyogenes in vitro . Based on these results , it is necessary to investigate the main antibacterial substance of bay leaves , as well as further studies in vivo and in humans.

Item Type:	Thesis (Sarjana)
Identification Number:	SKR/FK/2014/99/051401645
Subjects:	600 Technology (Applied sciences) > 610 Medicine and health
Depositing User:	Budi Wahyono Wahyono
Date Deposited:	05 Mar 2014 09:47
Last Modified:	22 Oct 2021 04:03
URI:	http://repository.ub.ac.id/id/eprint/124834

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