Pengaruh Endosulfan Terhadap Kadar Tnf-α Serum Pada Tikus Putih (Rattus norvegicus).

Riyu, Elita, (2014) Pengaruh Endosulfan Terhadap Kadar Tnf-α Serum Pada Tikus Putih (Rattus norvegicus). Sarjana thesis, Universitas Brawijaya.

Abstract

Endosulfan adalah insektisida organoklorin yang memiliki efek toksik. Centre for Science and the Environment (CSE) melaporkan kadar endosulfan pada sayuran, susu, tanah, dan air termasuk kategori tinggi pada tahun 2003. Penelitian Landrigan menunjukkan ibu hamil yang terpapar endosulfan selama periode organogenesis dapat mengganggu perkembangan sistem syaraf janin pada tahun 2007. Endosulfan juga dapat mengganggu sistem imun seperti pembentukan sitokin pro inflamasi dan autoimun. Tumor Necrosis Alfa (TNF-α) adalah salah satu sitokin proinflamasi yang terlibat dalam inflamasi sistemik. Tujuan penelitian ini adalah melihat pengaruh endosulfan terhadap kadar TNF-α serum pada anak tikus putih ( Rattus norvegicus ). Tikus betina yang sedang hamil dibagi menjadi 4 kelompok: kontrol (-), kontol (+) yang diberi endosulfan secara oral dengan dosis 0.07 ml, 0.33 ml, dan 1.6 ml. Pemaparan endosulfan dilakukan pada minggu kedua kehamilan tikus. Kemudian dilakukan pembedahan pada hari ke-30 setelah anak tikus dilahirkan, kemudian dilakukan pengukuran kadar TNF-α serum menggunakan ELISA kit. Analisis ANOVA menunjukkan paparan endosulfan selama kehamilan periode organogenesis terbukti secara signifikan meningkatkan kadar TNF-α serum anak tikus yaitu nilai p=0.000 (p < 0.05). Namun, perlu penelitian lebih lanjut untuk menentukan dosis maksimal dan menentukan pengaruh endosulfan terhadap sistem imun nduk tikus.

English Abstract

Endosulfan is an organochlorine insecticide which has toxic effects. Centre for Science and the Environment (CSE) reported that vegetables, milk, soil, and water are contaminated with endosulfan at high level category in 2003. Landrigan’s research showed that endosulfan can interfere the development of feotus nervous system during organogenesis at pregnancy in 2007. Endosulfan also can interfere immune system such as pro-inflammatory cytokines and autoimmunity. Tumor Necrosis Alpha (TNF-α) is one of pro-inflammatory cytokines which involved in systemic inflammation. This study intends to research the effects of endosulfan to TNF-alfa level at rats child blood serum. Pregnant rats are divided into 4 groups: control (-), control (+) is given endosulfan orally at different dose each group that is 0.07 ml, 0.33 ml, and 1.6 ml. Exposure of endosulfan was given at second week of pregnancy. Then the blood sample will be taken at 30th day after the rats are born and serum level of TNF-α are measured using ELISA kit. ANOVA analysis shows that exposure of endosulfan during organogenesis periode at pregnancy significantly increase the number of rats child TNF-α serum p=0.000 (P<0.05). However, more research is needed to determine the maximum dose and the effect of endosulfan on mother rats immune system.

Item Type: Thesis (Sarjana)
Identification Number: SKR/FK/2014/82/051401461
Subjects: 600 Technology (Applied sciences) > 610 Medicine and health > 610.7 Education, research, nursing, services of allied health personnel
Divisions: Fakultas Kedokteran > Pendidikan Dokter
Depositing User: Endang Susworini
Date Deposited: 26 Feb 2014 16:22
Last Modified: 22 Oct 2021 03:24
URI: http://repository.ub.ac.id/id/eprint/124816
[thumbnail of Bab_6.pdf]
Preview
Text
Bab_6.pdf

Download (1MB) | Preview
[thumbnail of Lampiran_1.pdf]
Preview
Text
Lampiran_1.pdf

Download (1MB) | Preview
[thumbnail of Lampiran_2.pdf]
Preview
Text
Lampiran_2.pdf

Download (1MB) | Preview
[thumbnail of Bab_7.pdf]
Preview
Text
Bab_7.pdf

Download (1MB) | Preview
[thumbnail of Cover+daftar_isi.pdf]
Preview
Text
Cover+daftar_isi.pdf

Download (1MB) | Preview
[thumbnail of Daftar_Pustaka.pdf]
Preview
Text
Daftar_Pustaka.pdf

Download (1MB) | Preview
[thumbnail of Lampiran_3.pdf]
Preview
Text
Lampiran_3.pdf

Download (2MB) | Preview
[thumbnail of Lampiran_4.pdf]
Preview
Text
Lampiran_4.pdf

Download (1MB) | Preview
[thumbnail of abstrak_bahasa_indonesia+inggris.pdf]
Preview
Text
abstrak_bahasa_indonesia+inggris.pdf

Download (1MB) | Preview
[thumbnail of Bab_1.pdf]
Preview
Text
Bab_1.pdf

Download (1MB) | Preview
[thumbnail of Bab_2.pdf]
Preview
Text
Bab_2.pdf

Download (1MB) | Preview
[thumbnail of Bab_3.pdf]
Preview
Text
Bab_3.pdf

Download (1MB) | Preview
[thumbnail of Bab_4.pdf]
Preview
Text
Bab_4.pdf

Download (1MB) | Preview
[thumbnail of Bab_5.pdf]
Preview
Text
Bab_5.pdf

Download (1MB) | Preview

Actions (login required)

View Item View Item