Pengaruh Ekstrak Kulit Manggis (Garcinia mangostana) Terhadap Fungsi Migrasi Human Umbilical Vein Endothelial Cells Yang Dipapar Lipopolisakarida

Jayanagara, Adianto (2014) Pengaruh Ekstrak Kulit Manggis (Garcinia mangostana) Terhadap Fungsi Migrasi Human Umbilical Vein Endothelial Cells Yang Dipapar Lipopolisakarida. Sarjana thesis, Universitas Brawijaya.

Abstract

Peran dari infeksi dipercaya bekerja sebagai stimulus inflamasi penting yang mengakibatkan penyakit kardiovaskuler. Lipopolisakarida (LPS), bagian utama pada lapisan luar membran sel bakteri gram-negatif, adalah penyebab utama inflamasi sel endotel. Penelitian ini dilakukan untuk mengamati pengaruh ekstrak kulit manggis (EKM) terhadap stres oksidatif akibat LPS, khususnya dalam menghambat penurunan migrasi Human Umbilical Vein Endothelial Cells (HUVECs) setelah dipapar LPS. Kultur HUVECs dibagi menjadi kelompok kontrol positif yang dipaparkan LPS 20 ng/ml, kelompok kontrol negatif yang tidak dipaparkan apapun, EKM 1, 2 dan 3 yang dipaparkan LPS 20 ng/ml dengan 1, 2 dan 4 µg/ml EKM. Pengamatan dilakukan setelah 6 dan 24 jam. Fungsi migrasi HUVECs diukur dengan uji migrasi scratch wound healing assay. Pemberian LPS mengakibatkan penurunan fungsi migrasi HUVECs. Pemberian EKM dapat menghambat penurunan fungsi migrasi HUVECs yang dipapar LPS pada dosis 1 µg/ml. Sedangkan didapatkan penurunan pada dosis 2 dan 4 µg/ml EKM.

English Abstract

The role of infection is believed to work as an important inflammatory stimulus that results in cardiovascular disease. Lipopolysaccharide (LPS), a major part of the outer layer of the cell membrane of gram-negative bacteria, is a major cause of endothelial cells inflammation. This study was conducted to investigate the effect of mangosteen peel extract (MPE) to oxidative stress due to LPS, especially in inhibiting the degradation of Human Umbilical Vein Endothelial Cells (HUVECs) migration function after being exposed to LPS. HUVECs culture were divided into positive group which exposed to LPS 20 ng/ml, negative control which not exposed with anything (normal physiology), MPE 1, 2 and 3 which exposed to LPS 20 ng/ml with 1, 2 and 4 µg/ml MPE. Observations were conducted after 6 and 24 hours. HUVECs migration function were measured by scratch wound healing assay. LPS exposure resulted in a decrease in HUVECs migration function. Addition of MPE can inhibit degradation of HUVECs migration function at dose of 1 µg/ml. While the reduction found at a dose of 2 and 4 µg/ml EKM.

Item Type: Thesis (Sarjana)
Identification Number: SKR/FK/2014/762/051501153
Subjects: 600 Technology (Applied sciences) > 610 Medicine and health > 610.7 Education, research, nursing, services of allied health personnel
Divisions: Fakultas Kedokteran > Pendidikan Dokter
Depositing User: Budi Wahyono Wahyono
Date Deposited: 16 Feb 2015 09:21
Last Modified: 22 Oct 2021 06:44
URI: http://repository.ub.ac.id/id/eprint/124789
[thumbnail of 2._Lembar_Pengesahan.pdf] Text
2._Lembar_Pengesahan.pdf

Download (1MB)
[thumbnail of 6._BAB_3.pdf] Text
6._BAB_3.pdf

Download (1MB)
[thumbnail of 7._BAB_4.pdf] Text
7._BAB_4.pdf

Download (1MB)
[thumbnail of 5._BAB_2.pdf] Text
5._BAB_2.pdf

Download (2MB)
[thumbnail of 3._Abstrak_Indonesia_+_inggris.pdf] Text
3._Abstrak_Indonesia_+_inggris.pdf

Download (1MB)
[thumbnail of 4._BAB_1.pdf] Text
4._BAB_1.pdf

Download (1MB)
[thumbnail of 9._BAB_6.pdf] Text
9._BAB_6.pdf

Download (1MB)
[thumbnail of 8._BAB_5.pdf] Text
8._BAB_5.pdf

Download (2MB)
[thumbnail of 10._BAB_7.pdf] Text
10._BAB_7.pdf

Download (1MB)
[thumbnail of 12._Lampiran.pdf] Text
12._Lampiran.pdf

Download (2MB)
[thumbnail of 11._Daftar_Pustaka.pdf] Text
11._Daftar_Pustaka.pdf

Download (1MB)
[thumbnail of 1._Cover.pdf] Text
1._Cover.pdf

Download (1MB)
[thumbnail of 1._Daftar_isi.pdf] Text
1._Daftar_isi.pdf

Download (1MB)
[thumbnail of Thumbnails conversion from text to thumbnail_lightbox] Other (Thumbnails conversion from text to thumbnail_lightbox)
lightbox.jpg

Download (44kB)
[thumbnail of Thumbnails conversion from text to thumbnail_preview] Other (Thumbnails conversion from text to thumbnail_preview)
preview.jpg

Download (18kB)
[thumbnail of Thumbnails conversion from text to thumbnail_medium] Other (Thumbnails conversion from text to thumbnail_medium)
medium.jpg

Download (4kB)
[thumbnail of Thumbnails conversion from text to thumbnail_small] Other (Thumbnails conversion from text to thumbnail_small)
small.jpg

Download (1kB)
[thumbnail of Thumbnails conversion from text to thumbnail_lightbox] Other (Thumbnails conversion from text to thumbnail_lightbox)
lightbox.jpg

Download (40kB)
[thumbnail of Thumbnails conversion from text to thumbnail_preview] Other (Thumbnails conversion from text to thumbnail_preview)
preview.jpg

Download (16kB)
[thumbnail of Thumbnails conversion from text to thumbnail_medium] Other (Thumbnails conversion from text to thumbnail_medium)
medium.jpg

Download (4kB)
[thumbnail of Thumbnails conversion from text to thumbnail_small] Other (Thumbnails conversion from text to thumbnail_small)
small.jpg

Download (1kB)
[thumbnail of Thumbnails conversion from text to thumbnail_lightbox] Other (Thumbnails conversion from text to thumbnail_lightbox)
lightbox.jpg

Download (40kB)
[thumbnail of Thumbnails conversion from text to thumbnail_preview] Other (Thumbnails conversion from text to thumbnail_preview)
preview.jpg

Download (16kB)
[thumbnail of Thumbnails conversion from text to thumbnail_medium] Other (Thumbnails conversion from text to thumbnail_medium)
medium.jpg

Download (4kB)
[thumbnail of Thumbnails conversion from text to thumbnail_small] Other (Thumbnails conversion from text to thumbnail_small)
small.jpg

Download (1kB)
[thumbnail of Thumbnails conversion from text to thumbnail_lightbox] Other (Thumbnails conversion from text to thumbnail_lightbox)
lightbox.jpg

Download (34kB)
[thumbnail of Thumbnails conversion from text to thumbnail_preview] Other (Thumbnails conversion from text to thumbnail_preview)
preview.jpg

Download (13kB)
[thumbnail of Thumbnails conversion from text to thumbnail_medium] Other (Thumbnails conversion from text to thumbnail_medium)
medium.jpg

Download (3kB)
[thumbnail of Thumbnails conversion from text to thumbnail_small] Other (Thumbnails conversion from text to thumbnail_small)
small.jpg

Download (1kB)
[thumbnail of Thumbnails conversion from text to thumbnail_lightbox] Other (Thumbnails conversion from text to thumbnail_lightbox)
lightbox.jpg

Download (32kB)
[thumbnail of Thumbnails conversion from text to thumbnail_preview] Other (Thumbnails conversion from text to thumbnail_preview)
preview.jpg

Download (13kB)
[thumbnail of Thumbnails conversion from text to thumbnail_medium] Other (Thumbnails conversion from text to thumbnail_medium)
medium.jpg

Download (3kB)
[thumbnail of Thumbnails conversion from text to thumbnail_small] Other (Thumbnails conversion from text to thumbnail_small)
small.jpg

Download (1kB)
[thumbnail of Thumbnails conversion from text to thumbnail_lightbox] Other (Thumbnails conversion from text to thumbnail_lightbox)
lightbox.jpg

Download (44kB)
[thumbnail of Thumbnails conversion from text to thumbnail_preview] Other (Thumbnails conversion from text to thumbnail_preview)
preview.jpg

Download (17kB)
[thumbnail of Thumbnails conversion from text to thumbnail_medium] Other (Thumbnails conversion from text to thumbnail_medium)
medium.jpg

Download (4kB)
[thumbnail of Thumbnails conversion from text to thumbnail_small] Other (Thumbnails conversion from text to thumbnail_small)
small.jpg

Download (1kB)
[thumbnail of Thumbnails conversion from text to thumbnail_lightbox] Other (Thumbnails conversion from text to thumbnail_lightbox)
lightbox.jpg

Download (32kB)
[thumbnail of Thumbnails conversion from text to thumbnail_preview] Other (Thumbnails conversion from text to thumbnail_preview)
preview.jpg

Download (13kB)
[thumbnail of Thumbnails conversion from text to thumbnail_medium] Other (Thumbnails conversion from text to thumbnail_medium)
medium.jpg

Download (3kB)
[thumbnail of Thumbnails conversion from text to thumbnail_small] Other (Thumbnails conversion from text to thumbnail_small)
small.jpg

Download (1kB)
[thumbnail of Thumbnails conversion from text to thumbnail_lightbox] Other (Thumbnails conversion from text to thumbnail_lightbox)
lightbox.jpg

Download (35kB)
[thumbnail of Thumbnails conversion from text to thumbnail_preview] Other (Thumbnails conversion from text to thumbnail_preview)
preview.jpg

Download (14kB)
[thumbnail of Thumbnails conversion from text to thumbnail_medium] Other (Thumbnails conversion from text to thumbnail_medium)
medium.jpg

Download (4kB)
[thumbnail of Thumbnails conversion from text to thumbnail_small] Other (Thumbnails conversion from text to thumbnail_small)
small.jpg

Download (1kB)
[thumbnail of Thumbnails conversion from text to thumbnail_lightbox] Other (Thumbnails conversion from text to thumbnail_lightbox)
lightbox.jpg

Download (40kB)
[thumbnail of Thumbnails conversion from text to thumbnail_preview] Other (Thumbnails conversion from text to thumbnail_preview)
preview.jpg

Download (16kB)
[thumbnail of Thumbnails conversion from text to thumbnail_medium] Other (Thumbnails conversion from text to thumbnail_medium)
medium.jpg

Download (4kB)
[thumbnail of Thumbnails conversion from text to thumbnail_small] Other (Thumbnails conversion from text to thumbnail_small)
small.jpg

Download (1kB)
[thumbnail of Thumbnails conversion from text to thumbnail_lightbox] Other (Thumbnails conversion from text to thumbnail_lightbox)
lightbox.jpg

Download (36kB)
[thumbnail of Thumbnails conversion from text to thumbnail_preview] Other (Thumbnails conversion from text to thumbnail_preview)
preview.jpg

Download (15kB)
[thumbnail of Thumbnails conversion from text to thumbnail_medium] Other (Thumbnails conversion from text to thumbnail_medium)
medium.jpg

Download (4kB)
[thumbnail of Thumbnails conversion from text to thumbnail_small] Other (Thumbnails conversion from text to thumbnail_small)
small.jpg

Download (1kB)
[thumbnail of Thumbnails conversion from text to thumbnail_lightbox] Other (Thumbnails conversion from text to thumbnail_lightbox)
lightbox.jpg

Download (44kB)
[thumbnail of Thumbnails conversion from text to thumbnail_preview] Other (Thumbnails conversion from text to thumbnail_preview)
preview.jpg

Download (18kB)
[thumbnail of Thumbnails conversion from text to thumbnail_medium] Other (Thumbnails conversion from text to thumbnail_medium)
medium.jpg

Download (4kB)
[thumbnail of Thumbnails conversion from text to thumbnail_small] Other (Thumbnails conversion from text to thumbnail_small)
small.jpg

Download (1kB)
[thumbnail of Thumbnails conversion from text to thumbnail_lightbox] Other (Thumbnails conversion from text to thumbnail_lightbox)
lightbox.jpg

Download (36kB)
[thumbnail of Thumbnails conversion from text to thumbnail_preview] Other (Thumbnails conversion from text to thumbnail_preview)
preview.jpg

Download (14kB)
[thumbnail of Thumbnails conversion from text to thumbnail_medium] Other (Thumbnails conversion from text to thumbnail_medium)
medium.jpg

Download (3kB)
[thumbnail of Thumbnails conversion from text to thumbnail_small] Other (Thumbnails conversion from text to thumbnail_small)
small.jpg

Download (1kB)
[thumbnail of Thumbnails conversion from text to thumbnail_lightbox] Other (Thumbnails conversion from text to thumbnail_lightbox)
lightbox.jpg

Download (49kB)
[thumbnail of Thumbnails conversion from text to thumbnail_preview] Other (Thumbnails conversion from text to thumbnail_preview)
preview.jpg

Download (20kB)
[thumbnail of Thumbnails conversion from text to thumbnail_medium] Other (Thumbnails conversion from text to thumbnail_medium)
medium.jpg

Download (4kB)
[thumbnail of Thumbnails conversion from text to thumbnail_small] Other (Thumbnails conversion from text to thumbnail_small)
small.jpg

Download (1kB)

Actions (login required)

View Item View Item