Wedana, IPutuGedeArya (2014) Efek Antimikroba Ekstrak Etanol Daun Efek Ciplukan (Physalis minima) terhadap Escherichia coli Secara In vitro. Sarjana thesis, Universitas Brawijaya.
Abstract
Diare yang disebabkan oleh Escherichia coli masih sering terjadi di Negara berkembang. Masyarakat yang tidak mengerti menggunakan antibiotik secara benar akan terus menggunakan antibiotik sembarangan untuk mengobati diare. Oleh karena itu diperlukan obat alternative. Salah satunya adalah daun ciplukan yang memiliki kandungan flavonoid dan saponin. Penelitian ini bertujuan untuk mengetahui ekstrak etanol daun ciplukan sebagai antimikroba terhadap Escherichia coli secara in vitro. Desain penelitian yang digunakan adalah eksperimental laboratorik dengan post test control group design only.metode yang digunakan adalah dilusi tabung yang digunakan untuk menentukan Kadar Hambat Minimum(KHM) dan penggunaan medium NAP untuk menentukan Kadar Bunuh Minumum (KBM).Ekstrak daun ciplukan dibuat dengan teknik dibuat dengan metode maserasi atau pengeringan lalu dicampurkan dengan etanol. konsentrasi ekstrak yang digunakan adalah 14%, 13%, 12%, 11%, dan 10%. dengan KHM 12% dan KBM 14%. Dengan membuktikan melalui Uji korelasi menunjukkan adanya hubungan antara konsentrasi ekstrak dengan jumlah koloni (Korelasi, R = -0.957: p <0,05). Berdasarkan hasil penelitian, dapat disimpulkan bahwa ekstrak etanol daun ciplukan memiliki efek antimikroba terhadap Escherchia coli secara in vitro.
English Abstract
Diarrhea caused by Escherichia coli is still common in developing countries. People who do not understand the correct usage of antibiotics will continue to use antibiotics in discriminately to treat diarrhea. Therefore, alternative medicines are necessary. One of them is ciplukan leaves containing flavonoid and saponin. This research aims to prove the ethanol extract of ciplukan leaves as antimicrobial against Escherichia coli in vitro. The design study used is laboratory experiment post test control group design only. The method used are tube dilution intended to determine levels of Minimum Inhibitory Concentration (MIC) and NAP medium to determine levels of Minimum Bactericidal Concentration (MBC). Ciplukan leaves extract is made from a method of maceration. The concentrations of extract used are 14%, 13%, 12%, 11%, and 10% with MIC and MBC of 12% and 14%. Through a correlation analytical study test, there is a relationship between the concentration of extract and the number of colony (of the correlation, R = -0.957: p<0.05). Based on the research results, it can be concluded that ethanol extract of ciplukan leaves have the effect of antimicrobial against Escherichia coli in vitro.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FK/2014/757/051501148 |
| Subjects: | 600 Technology (Applied sciences) > 610 Medicine and health > 610.7 Education, research, nursing, services of allied health personnel |
| Divisions: | Fakultas Kedokteran > Pendidikan Dokter |
| Depositing User: | Budi Wahyono Wahyono |
| Date Deposited: | 13 Feb 2015 15:05 |
| Last Modified: | 22 Oct 2021 06:40 |
| URI: | http://repository.ub.ac.id/id/eprint/124783 |
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