Laksmi, DyahAyu (2014) Efek Ekstrak Biji Pare (Momordica charantia) dalam Menghambat Peningkatan Kadar Resistin Serum pada Tikus (Rattus norvegicus) Wistar dengan Diet Aterogenik. Sarjana thesis, Universitas Brawijaya.
Abstract
Aterosklerosis terjadi akibat inflamasi kronis pada dinding pembuluh darah. Inflamasi ini melibatkan resistin yang berperan besar dalam progresivitas lesi. Sementara itu, saponin telah lama diteliti sebagai agen anti-aterosklerosis. Biji pare (Momordica charantia) yang masih memiliki daya guna rendah di Indonesia, terbukti mengandung saponin jenis triterpenoid. Namun, masih sangat jarang penelitian yang melibatkan saponin dari pare terhadap aterosklerosis. Penelitian ini bertujuan untuk membuktikan bahwa ekstrak biji pare yang diduga mengandung saponin dapat menghambat peningkatan kadar resistin serum. Studi true experimental dengan metode randomized posttest only controlled group design. Sebanyak 25 ekor tikus (Rattus norvegicus) Wistar jantan dibagi dalam 5 kelompok: K(+), KPA, KPB, dan KPC diberi diet aterogenik, serta K(-) diberi diet normal, masing-masing selama 12 minggu. Ekstrak biji pare diberikan mulai minggu 8 dengan dosis 150 μg/gBB (KPA), 300 μg/gBB (KPB), dan 500 μg/gBB (KPC). Setelah minggu 12, dilakukan ELISA dengan sampel serum. Uji Kruskal Wallis menunjukkan ada perbedaan bermakna kadar resistin serum antar kelompok (p < 0,05). Nilai koefisien korelasi (R) adalah -0,8516, berarti peningkatan dosis ekstrak biji pare berpengaruh negatif kuat terhadap kadar resistin serum. Uji Mann-Whitney menunjukkan kadar resistin K(+) lebih tinggi bermakna dibanding K(-) (p < 0,05), berarti pemberian diet aterogenik berpengaruh terhadap peningkatan kadar resistin serum. Dosis 150 μg/gBB dan 300 μg/gBB belum menampakkan penghambatan. Penghambatan resistin baru tampak pada dosis 500 μg/gBB, dibuktikan dengan uji Mann-Whitney yang menunjukkan tidak ada perbedaan bermakna antara kadar resistin K(-) dan KPC (p > 0,05). Kesimpulan penelitian ini adalah pemberian ekstrak biji pare (Momordica charantia) terbukti dapat menghambat peningkatan kadar resistin serum tikus Wistar dengan diet aterogenik. Perlu penelitian lebih lanjut untuk melihat pengaruh langsung ekstrak biji pare terhadap plak ateroma dan dinding pembuluh darah, uji toksisitas dan uji klinis ekstrak biji pare, serta pengembangan penelitian ke tingkat biomolekuler.
English Abstract
Atherosclerosis occurs as impact of chronic inflammation in blood vessel’s wall. This inflammation involves resistin which plays major roles in lesion progression. Meanwhile, saponin has long been studied as anti-atherosclerosis agent. Bitter melon (Momordica charantia) seed which still has low efficiency in Indonesia, has been proven containing triterpenoid-type saponin. However, there are very rare studies involving saponin from bitter melon against atherosclerosis. This study is aimed to prove that bitter melon seed extract which is tought to contain saponins can inhibit serum resistin levels. This true experimental study uses randomized posttest only controlled group design. 25 male Wistar rats (Rattus norvegicus) were divided in 5 groups: K(+), KPA, KPB, and KPC were given atherogenic diet, while K(-) was given normal diet for 12 weeks, respectively. Bitter melon seed extract was given from week 8 at doses 150 μg/gBW (KPA), 300 μg/gBW (KPB), and 500 μg/gBW (KPC). After week 12, ELISA was performed using serum as sample. Kruskal Wallis test showed significant differences in serum resistin levels among all groups (p < 0.05). Correlation coefficient value (R) is -0.8516, means an increase in dose of bitter melon seed extract had strong negative effect on serum resistin levels. Mann-Whitney test showed that resistin level of K(+) was significantly higher than K(-) (p < 0.05), which means atherogenic diet has effect on the increase of serum resistin levels. Doses 150 μg/gBW and 300 μg/gBW did not show any inhibition yet. Resistin inhibition was seen at dose 500 μg/gBW, proved by Mann-Whitney test which showed no significant difference between resistin levels of K(-) and KPC (p > 0.05). The conclusion is bitter melon seed extract was shown to inhibit serum resistin levels of Wistar rats with atherogenic diet. Advanced studies are needed to discover direct effect of bitter melon seed extract on atheroma plaque and vessel’s wall, toxicity and clinical rial of bitter melon seed extract, also biomolecular research development.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FK/2014/373/051403296 |
| Subjects: | 600 Technology (Applied sciences) > 610 Medicine and health > 610.7 Education, research, nursing, services of allied health personnel |
| Divisions: | Fakultas Kedokteran > Pendidikan Dokter |
| Depositing User: | Budi Wahyono Wahyono |
| Date Deposited: | 18 Jun 2014 11:49 |
| Last Modified: | 21 Oct 2021 10:01 |
| URI: | http://repository.ub.ac.id/id/eprint/124360 |
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