HEPAREGS (Hepar Regenerated by Stem Cells): Pengembangan Terapi Regeneratif Sebagai Alternatif Transplantasi pada Penyakit Sirosis Hepar dengan Menggunakan Ekstrak Oats (Avena sativa L.)

Susanto, DewanggaPrimananda (2014) HEPAREGS (Hepar Regenerated by Stem Cells): Pengembangan Terapi Regeneratif Sebagai Alternatif Transplantasi pada Penyakit Sirosis Hepar dengan Menggunakan Ekstrak Oats (Avena sativa L.). Sarjana thesis, Universitas Brawijaya.

Abstract

Sirosis hepar adalah inflamasi kronis pada hepar dengan berbagai macam penyebab serta menyebabkan terbentuknya jaringan fibrous yang akan membunuh sel hepar itu sendiri. Akibatnya hepar tidak berfungsi dengan baik, bahkan hepar akan mati. Penggunaan terapi regeneratif merupakan hal yang potensial untuk dikembangkan. Mengingat pengobatan sampai saat ini yang masih dianggap paling baik yaitu transplantasi, memiliki angka kegagalan yang cukup tinggi. Pemberian ekstrak oats yang banyak mengandung beta-glucan akan meningkatkan kadar G-CSF. Peningkatan kadar G-CSF dalam tubuh akan akan meningkatkan pelepasan hematopoietic stem cells dalam sirkulasi tubuh. Hematopoietic stem cells yang telah dilepaskan akan meregenerasi hepar yang telah rusak. Tujuan penelitian ini untuk mengetahui peran pemberian ekstrak oats dalam mengembalikan struktur anatomis dan fungsi fisiologis dari hepar. Tikus Wistar dibagi menjadi 5 kelompok: Kontrol negatif, Kontrol Positif, Perlakuan 1 (CCl4 + Ekstrak Oats 90mg/kgBB), Perlakuan 2 (CCl4 + Ekstrak Oats 180mg/kgBB), Perlakuan 3 (CCl4 + Ekstrak Oats 360mg/kgBB). Kelompok perlakuan diinjeksi CCL4 1mg/kgBB secara subcutaneus selama 8 minggu untuk menginduksi kerusakan hepar. Ekstrak oats diberikan setiap hari mulai minggu ke-3 hingga ke-8 pada kelompok perlakuan. Hasil penelitian menunjukan bahwa pemberian ekstrak oats mampu mengembalikan struktur anatomis hepar secara kualitatif. Pemberian ekstrak oats mampu menurunkan kadar SGOT, SGPT, dan bilirubin secara signifikan (p<0,05). Pemberian ekstrak oats juga mampu meningkatkan kadar protein total secara signifikan (p<0,05). Terdapat korelasi yang kuat dan signifikan (p<0,01) antara pemberian ekstrak oats dengan penurunan kadar SGOT (R=-0,879), kadar SGPT (R=-0,861), kadar bilirubin (R=-0,675) dan peningkatan kadar protein total (R=0,746). Kesimpulan dari penelitian ini yaitu ekstrak oats mampu mengembalikan struktur anatomis dan fungsi fisiologis dari hepar yang telah mengalami sirosis melalui mobilisasi hematopoietic stem cells dari sumsum tulang secara in vivo.

English Abstract

Liver cirrhosis is a chronic inflammation on liver that involves many factors. The continuous result of inflammation is associated with dying liver cells that causes fibrous tissues to form. As a result, the liver is not functioning properly, and eventually liver will die. Regenerative therapy is a potential therapy to be developed. While Liver Transplantation as the best therapy now still has a high failure rate. Oats contains betaglucan that can increase the level of G-CSF (Granulocyte Colony Stimulating Factor) in the body. Elevated levels of G-CSF would increase the release of hematopoietic stem cells into the body circulation. Hematopoietic stem cells will be mobilized into the liver and regenerate liver cells that have been damaged. The purpose of this study is to determine the effects of oats in restoring anatomical structures and physiological functions of the liver cirrhosis. 20 wistar male rats as experimental animals were divided into 5 groups: negative control, positive control, Treatment 1 (CCL4+Oats extraction 90mg/kgBB), Treatment 2 (CCL4+Oats extraction 180mg/kgBB), treatment 3 (CCL4+Oats extraction 360mg/kgBB). CCl4 to induce hepatic damage was injected subcutaneusly 1 ml/kg body weight for 8 weeks. Oats are given daily from week 3 to week 8. The results showed that administration of oats is able to restore the anatomical structure of the liver qualitatively. Oats could reduce the levels of SGOT, SGPT, and bilirubin significantly (p<0,05). Oats are able to increase the total protein levels significantly (p<0,05).There is a strong and significant correlation between the provision of oats with reduced levels of SGOT levels R=-0,879), SGPT levels (R=-0,861), bilirubin levels(R=-0,675) and elevated levels of total protein (R=0,746). Conclusion of this study shows oats can increase the mobilization of hematopoetic stem cells to regenerate liver cirrhosis, Indicated by the return of anatomical structures and physiological functions of the liver (decreased levels of SGOT, SGPT, total bilirubin, and increased levels of total protein in vivo). Thus, oats can become a regenerative therapy innovation for liver cirrhosis by mobilization of hematopoietic stem cells as an alternative for liver transplantation

Item Type: Thesis (Sarjana)
Identification Number: SKR/FK/2014/356/051403177
Subjects: 600 Technology (Applied sciences) > 610 Medicine and health > 610.7 Education, research, nursing, services of allied health personnel
Divisions: Fakultas Kedokteran > Pendidikan Dokter
Depositing User: Budi Wahyono Wahyono
Date Deposited: 11 Jun 2014 13:41
Last Modified: 21 Oct 2021 09:53
URI: http://repository.ub.ac.id/id/eprint/124343
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