Akib, VidiaMeiranda (2013) Uji Efektivitas Antimikroba Ekstrak Etanol Daun Kenanga (Cananga Odorata) Terhadap Bakteri Staphylococcus Aureus Secara In Vitro. Sarjana thesis, Universitas Brawijaya.
Abstract
Penyakit Infeksi Masih Merupakan Permasalahan Global Di Negara Berkembang Maupun Di Negara Maju. Antibiotik Awalnya Mampu Mengatasi Banyak Penyakit Infeksi. Kemudian Dijumpai Kuman Yang Resisten, Salah Satunya Staphylococcus Aureus. Daun Kenanga ( Cananga Odorata ) Memiliki Berbagai Khasiat. Penelitian Ini Bertujuan Untuk Mengetahui Efek Antimikroba Ekstrak Etanol Daun Kenanga Terhadap Bakteri Staphylococcus Aureus Secara In Vitro . Metode Yang Digunakan Adalah Uji Dilusi Tabung Untuk Penentuan Khm (Kadar Hambat Minimal) Dan Kbm (Kadar Bunuh Minimal). Konsentrasi Ekstrak Etanol Daun Kenanga Yang Digunakan Adalah 12% V/V, 13% V/V, 14% V/V, 15% V/V, Dan 16% V/V Serta Kontrol Positif Dan Kontrol Negatif Sebagai Pembanding. Analisis Statistik Yang Digunakan Adalah Uji Nonparametrik Kruskal Wallis Dengan Tingkat Kepercayaan 95% (Α = 0,05), Uji Post Hoc Mann-Whitney, Dan Korelasi Spearman . Dari Penelitian Ini Didapatkan: (1) Pertumbuhan Koloni Bakteri Yang Berbeda Secara Nyata Antara Paling Tidak Dua Konsentrasi Ekstrak Etanol Daun Kenanga ( Kruskal Wallis P = 0,000 [P < 0,05]), (2) Antara Perlakuan Berbagai Konsentrasi Terdapat Perbedaan Signifikan ( Mann-Whitney P < 0,05) (3) Uji Korelasi Spearman Didapatkan Bahwa Semakin Tinggi Konsentrasi Ekstrak Etanol Daun Kenanga Semakin Turun Jumlah Koloni Bakteri Yang Tumbuh (R = -0,989) (4) Khm Ditemukan Pada Konsentrasi 12% Dan Kbm Ditemukan Pada Konsentrasi 16%. Dari Penelitian Dapat Disimpulkan Bahwa Ekstrak Etanol Daun Kenanga Memiliki Efek Antimikroba Terhadap Bakteri Staphylococcus Aureus Secara In Vitro .
English Abstract
Infectious Disease Is Still A Global Issue Not Only In Developing Country But Also In Developed Country. The Antibiotic Was Able To Overcome A Lot Of Infectious Diseases But Resistance Of Bacteria Was Discovered For Example, Staphylococcus Aureus . Kenanga’s Leaf ( Cananga Odorata ) Had Abilities To Overcome Some Diseases. The Aim Of This Research Was To Discover The Antimicrobial Effect Of Kenanga’s Leaf Extract To Staphylococcus Aureus With In Vitro . This Study Used Tube Dilution Test Method To Find Mic (Minimum Inhibitory Concentration) And Mlc (Minimum Lethal Concentration). The Concentrations Of Kenanga Extract Used Were 12% V/V, 13% V/V, 14% V/V, 15% V/V, And 16% V/V. There Were Positive Control And Negative Control As Comparison. Statistical Analysis Used Was Nonparamateric Kruskal Wallis, Post Hoc Mann-Whitney, And Spearman Correlation. The Results Of This Study Were: (1) There Were Significant Different Of Colonies Growth At Least Between Two Extract Concentrations (Kruskal Wallis, P = 0,000 [P <0,05]), (2) There Was Significant Difference Between Each Consentration To Other (Mann-Whitney P<0,05), (3) The Higher Extract Concentration, The Lower Amount Of The Colonies Growth (Spearman Correlations Test, R = -0,989), (4) Mic Was Found Within 12% Extract Consentration And Mlc Was Found Within 16% Extract Concentration. Conclusions: Kenanga’s Leaf Extract Has Antimicrobial Effect To Staphylococcus Aureus With In Vitro Method.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FK/2013/616/051311654 |
| Subjects: | 600 Technology (Applied sciences) > 610 Medicine and health > 610.7 Education, research, nursing, services of allied health personnel |
| Divisions: | Fakultas Kedokteran > Pendidikan Dokter |
| Depositing User: | Budi Wahyono Wahyono |
| Date Deposited: | 11 Dec 2013 10:21 |
| Last Modified: | 21 Oct 2021 01:58 |
| URI: | http://repository.ub.ac.id/id/eprint/123907 |
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