Pamungkas, PrilianAS (2013) SACCREVACT (Saccharomyces cerevisiae As Chronic Kidney Failure Therapy) : Inovasi Pengobatan Penyakit Gagal Ginjal Kronis Berbasis Kedokteran Regeneratif Menggunakan Ragi Saccharomyces cerevisiae. Sarjana thesis, Universitas Brawijaya.
Abstract
Gagal ginjal kronis merupakan salah satu penyakit global yang serius dengan jumlah kematian 775.103 orang per tahun. Pengobatan yang ada hanya berupa transplantasi ginjal dan hemodialisis seumur hidup dimana sehingga dibutuhkan pengobatan yang bersifat non-invasif dan memiliki efektifitas yang baik. Di lain sisi ragi Saccharomyces cerevisiae yang sering digunakan pada pembuatan roti memiliki kandungan beta-glucan dimana kandungan tersebut mampu meningkatkan pelepasan Hematopoeitic stem cells (HSC) dari sumsum tulang yang akan beredar dalam darah dan menuju ginjal. Penelitian ini dilakukan dengan tujuan untuk membuktikan efek pemberian ragi Saccharomyces cerevisiae dalam memobilisasi HSC dari sumsum tulang belakang ke ginjal mencit model gagal ginjal kronis. Penelitian ini merupakan eksperimen murni dengan rancangan Randomized Post Test Only Controlled Group Design. Mencit balb/c jantan diinduksi CCL4 (carbon tetrachloride) 0,5 ml/g BB untuk dibuat keadaan gagal ginjal kronis dan dibagi menjadi 5 kelompok yaitu: Kontrol negatif (tanpa perlakuan), kontrol positif (induksi CCL4), P1 (CCL4 + yeast 50mg/kgBB), P2 (CCL4 + yeast 100mg/kgBB), P3 (CCL4 + yeast 200mg/kgBB) dengan induksi CCL4 selama 12 minggu dan yeast mulai minggu ke-7 sampai 12. Hasil penelitian menunjukkan pemberian ragi Saccharomyces cerevisiae mampu meningkatkan persentase CD34+ pada sumsum tulang p=0,000 (p<0.05), meningkatkan persentase CD34+ pada ginjal p=0,000 (p<0.05). Dengan demikian, disimpulkan bahwa pemberian ragi Saccharomyces cerevisiae mampu meningkatkan produksi dan mobilisasi HSC dari sumsum tulang belakang ke ginjal pada mencit model gagal ginjal kronis.
English Abstract
Chronic renal disease is one of the serious global disease with a mortality rate of 775.103 deaths each year. Current therapy are renal transplantation and lifetime hemodialysis, so these lead to the need of non-invasive and effective treatment. On the other side, the Saccharomyces cerevisiae yeast which is common in bread-making, contains beta-glucan which is able to increase the Hematopoietic Stem Cell (HSC) release from bone marrow to blood circulation then addressed to renal organ. This research is conducted to prove that Saccharomyces cerevisiae yeast has an impact on HSC mobilization to renal organ of chronic renal failure balb/c mice model. This research is true experimental model with Randomized Post Test Only Controlled Group Design. Male Balb/c mice are inducted by CCL4 (carbon tetrachloride) 0.5 mL/BW gr to make chronic renal failure model then divided into 5 groups : Negative control (no intervention), Positive control (inducted by CCL4), P1 (CCL4 + yeast 50mg/BW kg), P2 (CCL4 + yeast 100mg/BW kg), P3 (CCL4 + yeast 200mg/BW kg) with 12 weeks CCl4 induction and yeast given start from 7th to 12th week. The results show that Saccharomyces cerevisiae could improve the percentage of CD34+ on bone marrow p=0.000 (p<0.05), and improving the percentage of CD34+ on renal organ p=0.000 (p<0.05). From these results, it can be concluded that Saccharomyces cerevisiae could improve the production and mobilization of HSC in bone marrow to renal organ on chronic renal failure Balb/c mice model.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FK/2013/567/051309559 |
| Subjects: | 600 Technology (Applied sciences) > 610 Medicine and health > 610.7 Education, research, nursing, services of allied health personnel |
| Divisions: | Fakultas Kedokteran > Pendidikan Dokter |
| Depositing User: | Hasbi |
| Date Deposited: | 23 Oct 2013 09:40 |
| Last Modified: | 20 Oct 2021 12:25 |
| URI: | http://repository.ub.ac.id/id/eprint/123856 |
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