Efek Ekstrak Kacang Tunggak (Vigna unguiculata) Terhadap kadar Malondialdehide (MDA) paru tikus galur wistar (Rattus Novergivus) pada berbagai macam lama paparan asap kendaraan bermotor.

Kapisa, ObiChandra (2013) Efek Ekstrak Kacang Tunggak (Vigna unguiculata) Terhadap kadar Malondialdehide (MDA) paru tikus galur wistar (Rattus Novergivus) pada berbagai macam lama paparan asap kendaraan bermotor. Sarjana thesis, Universitas Brawijaya.

Abstract

Asap kendaraan bermotor mengandung senyawa-senyawa polutan yang merupakan radikal bebas. Tingginya kadar polutan yang terinhalasi atau lamanya waktu paparan asap dapat menyebabkan kondisi stres oksidatif yang memicu peningkatan kadar malondialdehide (MDA). Kacang tunggak (Vigna unguiculata) berdasarkan kajian teoritis mengandung senyawa genistein yang dapat bekerja sebagai antioksidan dan antiinflamasi sehingga mampu mencegah terjadi peningkatan kadar malondialdehide (MDA). Tujuan penelitian ini adalah untuk mengetahui pengaruh pemberian ekstrak kacang tunggak terhadap kadar malondialdehide paru tikus wistar (Rattus norvegicus) yang dipapar asap kendaraan bermotor. Penelitian eksperimental ini menggunakan tiga puluh enam tikus wistar jantan yang dibagi secara random dalam 9 kelompok, terdiri dari kelompok normal (-) , kelompok yang diberi ekstrak kacang tunggak (+) G, Kelompok yang diberi oksigen 4 menit (+) O2, kelompok yang diberi perlakuan asap 2,3 dan 4 menit , masing-masing diberikan oksigen 4 menit dengan ekstrak kacang tunggak (A2 O4 (+) G, A3 O4 (+) G, A4 O4 (+) G) dan tanpa ekstrak kacang tunggak (A2O4(-)G, A3O4(-)G, A4O4(-)G). Untuk mengetahui kadar MDA paru yang merupakan hasil peroksidasi lemak akibat aktivitas radikal bebas, digunakan metode thiobarbituric acid (TBA). Berdasarkan uji Kruskall Wallis didapatkan nilai p < 0,05 untuk kadar MDA paru tikus. .Pada hasil Analisis post hoc dengan menggunakan uji Mann whitney menunjukkan adanya penurunan yang signifikan dari kadar Malondialdehide pada Kelompok A2O4(+)G, A3O4(+)G, A4O4(+)G dibandingkan dengan Kelompok A2O4(-)G, A3O4(-)G, A4O4(-)G. Kesimpulan dari penelitian ini adalah ekstrak kacang tunggak dapat mencegah peningkatan kadar Malondialdehide (MDA) paru tikus galur wistar (Rattus novergicus) yang dipapar asap kendaraan bermotor pada berbagai macam lama paparan.

English Abstract

Vehicle exhaust smoke contains various compounds such as free radical pollutants. High levels of pollutants that were inhaled or length of exhaust smoke exposure time could cause oxidative stress conditions that triggered an increase in malondialdehide (MDA) level. Based on the theoretical study, cowpea (Vigna unguiculata) contains genistein that work as antioxidants and anti-inflammatory to prevent an increase in the level of malondialdehide (MDA). The purpose of this study was to determine the effect of cowpea extract against lung malondialdehide level given to Wistar rats (Rattus norvegicus) which had been exposed to vehicle exhaust smoke. This experimental study used Thirty-six male Wistar rats randomly divided in 9 groups, ranging from the normal group (-), the group given the extract of cowpea (+) G, group given oxygen 4 minutes (+) O2, group given exhaust smoke with 2,3 and 4 minutes, each given 4 minutes oxygen with cowpea extract (A2 O4 (+) G, A3 O4 (+) G, A4 O4 (+) G) and without cowpea extract (A2O4 (-) G, A3O4 (-) G, A4O4 (-) G). Observation of MDA level which as result of fat peroxidation due to free radical activity was done using thiobarbituric acid (TBA) method. Kruskall Wallis test resulted p <0.05 for MDA rat lung. During post hoc analysis of the results using the Mann Whitney test showed that there was significant reduction in the level of Malondialdehide on group A2O4 (+) G, A3O4 (+) G, A4O4 (+) compared to group G A2O4 (-) G, A3O4 (- ) G, A4O4 (-) G. Conclusion from this study is cowpea extract can prevent increase of Malondialdehide (MDA) lung on wistar rat (Rattus novergicus) which had been exposed with vehicle exhaust smoke on various exposure time.

Item Type: Thesis (Sarjana)
Identification Number: SKR/FK/2013/426/051305830
Subjects: 600 Technology (Applied sciences) > 610 Medicine and health > 610.7 Education, research, nursing, services of allied health personnel
Divisions: Fakultas Kedokteran > Pendidikan Dokter
Depositing User: Hasbi
Date Deposited: 30 Jul 2013 09:42
Last Modified: 19 Oct 2021 01:12
URI: http://repository.ub.ac.id/id/eprint/123709
[thumbnail of Cover.pdf] Text
Cover.pdf

Download (1MB)
[thumbnail of LAMPIRAN.pdf] Text
LAMPIRAN.pdf

Download (3MB)
[thumbnail of DAFTAR_PUSTAKA.pdf] Text
DAFTAR_PUSTAKA.pdf

Download (1MB)
[thumbnail of ABSTRAK.pdf] Text
ABSTRAK.pdf

Download (1MB)
[thumbnail of BAB_1.pdf] Text
BAB_1.pdf

Download (1MB)
[thumbnail of BAB_2.pdf] Text
BAB_2.pdf

Download (3MB)
[thumbnail of BAB_3.pdf] Text
BAB_3.pdf

Download (1MB)
[thumbnail of BAB_4.pdf] Text
BAB_4.pdf

Download (2MB)
[thumbnail of BAB_5.pdf] Text
BAB_5.pdf

Download (1MB)
[thumbnail of BAB_6.pdf] Text
BAB_6.pdf

Download (1MB)
[thumbnail of BAB_7.pdf] Text
BAB_7.pdf

Download (1MB)
[thumbnail of Thumbnails conversion from text to thumbnail_lightbox] Other (Thumbnails conversion from text to thumbnail_lightbox)
lightbox.jpg

Download (37kB)
[thumbnail of Thumbnails conversion from text to thumbnail_preview] Other (Thumbnails conversion from text to thumbnail_preview)
preview.jpg

Download (15kB)
[thumbnail of Thumbnails conversion from text to thumbnail_medium] Other (Thumbnails conversion from text to thumbnail_medium)
medium.jpg

Download (4kB)
[thumbnail of Thumbnails conversion from text to thumbnail_small] Other (Thumbnails conversion from text to thumbnail_small)
small.jpg

Download (1kB)
[thumbnail of Thumbnails conversion from text to thumbnail_lightbox] Other (Thumbnails conversion from text to thumbnail_lightbox)
lightbox.jpg

Download (40kB)
[thumbnail of Thumbnails conversion from text to thumbnail_preview] Other (Thumbnails conversion from text to thumbnail_preview)
preview.jpg

Download (16kB)
[thumbnail of Thumbnails conversion from text to thumbnail_medium] Other (Thumbnails conversion from text to thumbnail_medium)
medium.jpg

Download (4kB)
[thumbnail of Thumbnails conversion from text to thumbnail_small] Other (Thumbnails conversion from text to thumbnail_small)
small.jpg

Download (1kB)
[thumbnail of Thumbnails conversion from text to thumbnail_lightbox] Other (Thumbnails conversion from text to thumbnail_lightbox)
lightbox.jpg

Download (41kB)
[thumbnail of Thumbnails conversion from text to thumbnail_preview] Other (Thumbnails conversion from text to thumbnail_preview)
preview.jpg

Download (16kB)
[thumbnail of Thumbnails conversion from text to thumbnail_medium] Other (Thumbnails conversion from text to thumbnail_medium)
medium.jpg

Download (4kB)
[thumbnail of Thumbnails conversion from text to thumbnail_small] Other (Thumbnails conversion from text to thumbnail_small)
small.jpg

Download (1kB)
[thumbnail of Thumbnails conversion from text to thumbnail_lightbox] Other (Thumbnails conversion from text to thumbnail_lightbox)
lightbox.jpg

Download (32kB)
[thumbnail of Thumbnails conversion from text to thumbnail_preview] Other (Thumbnails conversion from text to thumbnail_preview)
preview.jpg

Download (13kB)
[thumbnail of Thumbnails conversion from text to thumbnail_medium] Other (Thumbnails conversion from text to thumbnail_medium)
medium.jpg

Download (3kB)
[thumbnail of Thumbnails conversion from text to thumbnail_small] Other (Thumbnails conversion from text to thumbnail_small)
small.jpg

Download (1kB)
[thumbnail of Thumbnails conversion from text to thumbnail_lightbox] Other (Thumbnails conversion from text to thumbnail_lightbox)
lightbox.jpg

Download (41kB)
[thumbnail of Thumbnails conversion from text to thumbnail_preview] Other (Thumbnails conversion from text to thumbnail_preview)
preview.jpg

Download (16kB)
[thumbnail of Thumbnails conversion from text to thumbnail_medium] Other (Thumbnails conversion from text to thumbnail_medium)
medium.jpg

Download (4kB)
[thumbnail of Thumbnails conversion from text to thumbnail_small] Other (Thumbnails conversion from text to thumbnail_small)
small.jpg

Download (1kB)
[thumbnail of Thumbnails conversion from text to thumbnail_lightbox] Other (Thumbnails conversion from text to thumbnail_lightbox)
lightbox.jpg

Download (35kB)
[thumbnail of Thumbnails conversion from text to thumbnail_preview] Other (Thumbnails conversion from text to thumbnail_preview)
preview.jpg

Download (15kB)
[thumbnail of Thumbnails conversion from text to thumbnail_medium] Other (Thumbnails conversion from text to thumbnail_medium)
medium.jpg

Download (4kB)
[thumbnail of Thumbnails conversion from text to thumbnail_small] Other (Thumbnails conversion from text to thumbnail_small)
small.jpg

Download (1kB)
[thumbnail of Thumbnails conversion from text to thumbnail_lightbox] Other (Thumbnails conversion from text to thumbnail_lightbox)
lightbox.jpg

Download (35kB)
[thumbnail of Thumbnails conversion from text to thumbnail_preview] Other (Thumbnails conversion from text to thumbnail_preview)
preview.jpg

Download (14kB)
[thumbnail of Thumbnails conversion from text to thumbnail_medium] Other (Thumbnails conversion from text to thumbnail_medium)
medium.jpg

Download (3kB)
[thumbnail of Thumbnails conversion from text to thumbnail_small] Other (Thumbnails conversion from text to thumbnail_small)
small.jpg

Download (1kB)
[thumbnail of Thumbnails conversion from text to thumbnail_lightbox] Other (Thumbnails conversion from text to thumbnail_lightbox)
lightbox.jpg

Download (43kB)
[thumbnail of Thumbnails conversion from text to thumbnail_preview] Other (Thumbnails conversion from text to thumbnail_preview)
preview.jpg

Download (17kB)
[thumbnail of Thumbnails conversion from text to thumbnail_medium] Other (Thumbnails conversion from text to thumbnail_medium)
medium.jpg

Download (4kB)
[thumbnail of Thumbnails conversion from text to thumbnail_small] Other (Thumbnails conversion from text to thumbnail_small)
small.jpg

Download (1kB)
[thumbnail of Thumbnails conversion from text to thumbnail_lightbox] Other (Thumbnails conversion from text to thumbnail_lightbox)
lightbox.jpg

Download (41kB)
[thumbnail of Thumbnails conversion from text to thumbnail_preview] Other (Thumbnails conversion from text to thumbnail_preview)
preview.jpg

Download (17kB)
[thumbnail of Thumbnails conversion from text to thumbnail_medium] Other (Thumbnails conversion from text to thumbnail_medium)
medium.jpg

Download (4kB)
[thumbnail of Thumbnails conversion from text to thumbnail_small] Other (Thumbnails conversion from text to thumbnail_small)
small.jpg

Download (1kB)
[thumbnail of Thumbnails conversion from text to thumbnail_lightbox] Other (Thumbnails conversion from text to thumbnail_lightbox)
lightbox.jpg

Download (37kB)
[thumbnail of Thumbnails conversion from text to thumbnail_preview] Other (Thumbnails conversion from text to thumbnail_preview)
preview.jpg

Download (15kB)
[thumbnail of Thumbnails conversion from text to thumbnail_medium] Other (Thumbnails conversion from text to thumbnail_medium)
medium.jpg

Download (4kB)
[thumbnail of Thumbnails conversion from text to thumbnail_small] Other (Thumbnails conversion from text to thumbnail_small)
small.jpg

Download (1kB)
[thumbnail of Thumbnails conversion from text to thumbnail_lightbox] Other (Thumbnails conversion from text to thumbnail_lightbox)
lightbox.jpg

Download (45kB)
[thumbnail of Thumbnails conversion from text to thumbnail_preview] Other (Thumbnails conversion from text to thumbnail_preview)
preview.jpg

Download (17kB)
[thumbnail of Thumbnails conversion from text to thumbnail_medium] Other (Thumbnails conversion from text to thumbnail_medium)
medium.jpg

Download (4kB)
[thumbnail of Thumbnails conversion from text to thumbnail_small] Other (Thumbnails conversion from text to thumbnail_small)
small.jpg

Download (1kB)

Actions (login required)

View Item View Item