Rochmawati, IcmiDian (2012) Pengaruh Pemberian Jus Buah Melon (Cucumis Melo L.) Terhadap Aktivitas Enzim Superoksida Dismutase (SOD) Hepar Tikus Putih Galur Wistar )Rattus Novergicus) yang diinduksi Karbontetraklorida (CCl4). Sarjana thesis, Universitas Brawijaya.
Abstract
Kerusakan organ hepar merupakan salah satu penyebab kematian paling banyak di dunia dan paling sering disebabkan karena peroksidasi lipid dan kerusakan oksidatif yang berkaitan erat dengan radikal bebas, stress oksidatif dan penurunan antioksidan endogen. CCl 4 (karbon tetraklorida) menyebabkan kerusakan hepar dengan mekanisme pembentukan radikal bebas dan peroksidasi lipid. Buah Melon (Cucumis melo L.) mengandung antioksidan seperti vitamin C dan β-karoten diduga mampu menangkal radikal bebas. Tujuan penelitian adalah mengetahui pengaruh jus buah melon terhadap aktivitas enzim SOD (superoksida diamutase) hepar tikus yang diinduksi CCl 4 . Studi eksperimental menggunakan Post Test Only Control Group dilakukan pada 25 hewan coba tikus putih wistar jantan (Rattus Novergicus) yang dibagi menjadi lima kelompok terdiri dari kontrol negatif (normal), kontrol positif (diinduksi CCl 4 ) dan tiga kelompok perlakuan (diinduksi CCl 4 dan dilanjutkan pemberian jus melon masing-masing 2ml/hari, 4ml/hari, 6 ml/hari peroral). Variabel yang diukur adalah aktivitas enzim SOD (metode pewarna formazen dengan pembacaan spektrofotometri). Hasil penelitian didapatkan aktivitas Enzim SOD turun signifikan akibat induksi CCl 4. Semua dosis jus melon dapat meningkatkan aktivitas enzim SOD hepar tikus, terutama dosis 6 ml/hari yang mampu meningkatkan aktivitas SOD secara signifikan (p=0.000) dan mendekati normal (p=0.107). Kesimpulan penelitian adalah pemberian jus buah melon (Cucumis melo L.) dapat meningkatan aktivitas enzim SOD hepar tikus putih galur wistar yang diinduksi CCl 4 dan dosis efektifnya adalah 6 ml/hari.
English Abstract
liver damage is one of the most frequent death cause in the world, and most of them are caused by lipid peroxidation and oxidative damage which is related strongly to free radicals, stress oxidative, and decrease of antioxidant endogenous activity. CCl 4 (Carbon tetrachloride) cause liver damage with formation of free radicals and lipid peroxidation mechanism. Melon fruit (Cucumis melo L.) contains antioxidant like Vitamin C and β-carotene which is presumed for having free radicals scavenging action. This research was aimed to determine the effect of melon fruit juice on superoxide dismutase enzyme activity on liver rats induced CCl 4 . Experimental study using Post Test Only Control Group design conducted onto 25 male rats wistar strain (Rattus novergius) divided into five groups , that were negative control (normal), positive control (induced CCl 4 ) and three groups treatment (induced CCl 4 and treated with melon juice 2ml/day, 4 ml/day, 6ml/day orally for every group). The Variable measured was SOD enzyme activity (estimated using formazen method with spectrophotometer). The result of SOD activity on liver rats induced CCl 4 decreased significantly. All of dose of melon fruit juice could increase SOD activity on liver rats, especially on dose 6 ml/day which could increase SOD activity significantly (p=0.000) and approach the normal value (p=0.107). the conclusion of this research is melon fruit juice can increase on superoxide dismutase enzyme activity on liver rats induced CCl 4 and the effective dose is 6 ml/day.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FK/2012/416/051301938 |
| Subjects: | 600 Technology (Applied sciences) > 610 Medicine and health > 610.7 Education, research, nursing, services of allied health personnel |
| Divisions: | Fakultas Kedokteran > Pendidikan Dokter |
| Depositing User: | Endang Susworini |
| Date Deposited: | 11 Mar 2013 15:27 |
| Last Modified: | 19 Oct 2021 03:27 |
| URI: | http://repository.ub.ac.id/id/eprint/123341 |
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