Chandra, HarriKurnia (2012) Efek Ekstrak Etanol Biji Buah Delima (Punica granatum L.) Terhadap Penghambatan Pertumbuhan Jamur Candida albicans Secara In Vitro. Sarjana thesis, Universitas Brawijaya.
Abstract
Candida albicans ( C. albicans ) merupakan fungi patogen utama penyebab kejadian mikosis akibat genus Candida (kandidiasis). Studi penelitian pada masyarakat menemukan angka prevalensi kandidiasis yang sangat tinggi dibanding dengan penyebab infeksi saluran reproduksi lainnya, yaitu sebesar 57% . Meskipun pilihan terapi yang tersedia tampak efektif, banyak penduduk Indonesia yang lebih memilih bahan alami untuk mengatasi kandidiasis. Salah satu alternatif terapi adalah dengan bahan alami, yaitu biji buah delima ( Punica granatum L. Kandungan aktif biji buah yang diduga bermanfaat sebagai antimikroba adalah ellagid acid, punicalagin dan flavonoid . Penelitian ini merupakan langkah awal untuk membuktikan efek antifungi ekstrak biji buah delima terhadap pertumbuhan jamur Candida albicans secara in vitro. Studi eksperimental berupa post test only control group design dilakukan terhadap Candida albicans dengan metode dilusi tabung. Kelompok kontrol tidak diberi ekstrak (0%) sedangkan kelompok perlakuan diberi ekstrak biji buah delima dengan dosis 22.5%, 25%, 27.5%, 30%, 32.5%, 35% dan 37.5%. Dari hasil penelitian, didapatkan bahwa KHM dan KBM ekstrak berturut – turut adalah 32.5% dan 37.5%. Berdasarkan uji statistik, diperoleh bahwa terdapat perbedaan yang bermakna antara dosis ekstrak biji buah delima dengan pertumbuhan koloni Candida albicans (ANOVA p<0,05). Terdapat hubungan yang sangat erat dan berkebalikan antara dosis ekstrak dengan jumlah koloni yang tumbuh (Uji Regresi Linier R = -0.940). Oleh karena itu, dapat disimpulkan bahwa ekstrak biji buah delima mampu menghambat pertumbuhan Candida albicans secara signifikan .
English Abstract
Candida albicans ( C. albicans ) is the main etiologic agent of mycoses due to Candida genus ( candidiasis ). Past study has reported high candidiasis prevalence in society, which stated that candidiasis was responsible for 57% of infection on reproductive system. Much adequate treatments availabled for candidiasis, but a lot of Indonesian people still prefered to use natural ingredients to overcome the disease. One of the natural alternative therapy that could be used is extract of pomegranate seed ( Punica granatum L. ). The active compositions of pomegranate seed which was allegedly useful as antimicrobial were ellagic acid , punicalagin and flavonoid . This research was conducted to prove the antifungal effect of pomegranate seed extract to the growth of Candida albicans in vitro. This experimental study was using post-test only control group design with tube dilution method. The control group was given no extract (0%), while the trial groups were given the extract of pomegranate seed with variable doses, such as 22.5%, 25%, 27.5%, 30%, 32.5%, 35% and 37.5%. The result indicated that the pomegranate seed extract has antifungal properties, with MIC (Minimun Inhibition Concentration) and MBC (Minimum Bactericidal Concentration) are 32.5% and 37.5% respectively. Statistical analysis showed that there was significant difference between various doses of pomegranate seed extract and the growth of Candida albicans . (ANOVA p< 0.05). There was very strong and negative correlation between dose of promegranate seed extract and growth of fungi (Regression correlation test R = -0.940). This experiment brought up a conclusion that pomegranate seed extract has antifungal effect to Candida albicans reflected by the inhibition of fungal growth.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FK/2012/385/051300895 |
| Subjects: | 600 Technology (Applied sciences) > 610 Medicine and health > 610.7 Education, research, nursing, services of allied health personnel |
| Divisions: | Fakultas Kedokteran > Pendidikan Dokter |
| Depositing User: | Endang Susworini |
| Date Deposited: | 13 Feb 2013 09:36 |
| Last Modified: | 19 Oct 2021 03:09 |
| URI: | http://repository.ub.ac.id/id/eprint/123309 |
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