Dewi, Putri (2017) Efek Terapi Kombinasi Kurkumin Dan Vitamin E Terhadap Ekspresi Interleukin-1 (Il-1) Dan Cyclooxygenase-2 (Cox-2) Pada Ovarium Tikus (Rattus Norvegicus) Model Kanker Mammae Hasil Induksi Dmba (7,12 Dimethyl Benz (Α) Anthracene). Sarjana thesis, Universitas Brawijaya.
Abstract
Kanker mammae merupakan suatu kelompok neoplasma ganas yang menyerang jaringan epitel payudara yang dapat dipicu oleh agen karsinogenik DMBA (dimethyl benz(α) anthracene). Kanker mammae akibat induksi DMBA mempunyai kemungkinan untuk menyebar ke organ lain seperti ovarium. Peningkatan jumlah ROS secara sistemik dapat menimbulkan kondisi stress oksidatif pada ovarium, hal ini memicu makrofag untuk mengaktivasi sitokin proinflamatori, yaitu IL-1 dan COX-2 untuk menimbulkan reaksi inflamasi. Tujuan penelitian ini adalah untuk mengetahui efek terapi kombinasi kurkumin dengan vitamin E dalam menurunkan ekspresi IL-1 dan COX-2 pada ovarium tikus model kanker mammae yang diinduksi oleh DMBA. Pembuatan hewan model kanker mammae dilakukan dengan induksi DMBA dosis 10 mg/kg BB dan estrogen dosis 20.000 IU/kg BB. Penelitian ini dibagi dalam 5 kelompok perlakuan yaitu kelompok kontrol negatif (KN), kontrol positif (KP), perlakuan satu (P1) terapi dengan kurkumin 48mg/kg BB dan vitamin E 300 IU/ekor, perlakuan dua (P2) dosis 72mg/kg BB dan 200 IU/ekor, dan perlakuan tiga (P3) dosis 108mg/kg BB dan 100 IU/ekor. Ekspresi IL-1 dan COX-2 diamati dengan Imunohistokimia. Analisis data dilakukan dengan uji ANOVA, karena terdapat perbedaan nyata hasil antar perlakuan, maka dilakukan uji Tukey (α<0,05). Hasil penelitian menunjukkan terapi kombinasi kurkumin dan vitamin E pada tikus kanker mammae mampu menurunkan ekspresi IL-1 sebesar 60% dan COX-2 sebesar 43% secara signifikan dengan dosis efektif kurkumin 108 mg/kg BB dan vitamin E 100 IU/ekor.
English Abstract
Mammary cancer is a group of malignant neoplasms that attack the breast epithelial tissue that can be triggered by DMBA carcinogenic agents (dimethyl benz (α) anthracene). Mammary cancer due to DMBA induction has the possibility to spread to other organs such as ovaries. A systemic increase in ROS may cause oxidative stress conditions in the ovaries, which triggers macrophages to activate proinflammatory cytokines, IL-1 and COX-2 to induce an inflammatory reaction. The purpose of this study was to determine the effect of combination therapy of curcumin with vitamin E in reducing expression of IL-1 and COX-2 in rats mammary cancer model resulted from DMBA-induced mammary cancer. The development of mammary cancer animal model was performed by DMBA induction dose of 10 mg / kg BW and estrogen dose of 20.000 IU / kg BW. The study was divided into 5 treatment groups, negative control group (KN), positive control (KP), first treatment (P1) with therapy curcumin of 48 mg/kg BW and vitamin E of 300 IU / rat, second treatment (P2) of 72 mg/kg BW and 200 IU/rat, and third treatment (P3) with doses of 108mg/kg BW and 100 IU/rat. Expressions of IL-1 and COX-2 were observed with Immunohistochemistry. Data analysis was done by ANOVA test, since there was significant difference between treatment result, Tukey test (α<0,05) was done. The results of the combination group study of curcumin and vitamin E in mammary rats reduced the expression of IL-1 by 60% and COX-2 by 43% significantly with effective dose curcumin of 108 mg/kg BW and vitamin E of 100 IU/rat.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FK/2017/201/051800324 |
| Uncontrolled Keywords: | Kanker Mammae, Ovarium, DMBA, IL-1, COX-2, Kurkumin, Vitamin E, Mammary cancer, Ovaries, DMBA, IL-1, COX-2, Curcumin, Vitamin E. |
| Subjects: | 600 Technology (Applied sciences) > 616 Diseases > 616.9 Other disease > 616.99 Tumors and miscellaneous communicable diseases > 616.994 Cancers > 616.994 49 Breast cancer |
| Divisions: | Fakultas Kedokteran Hewan > Kedokteran Hewan |
| Depositing User: | Nur Cholis |
| Date Deposited: | 22 Jan 2018 07:07 |
| Last Modified: | 24 Oct 2021 05:38 |
| URI: | http://repository.ub.ac.id/id/eprint/8276 |
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