Dwihantari, Ajeng Rizki (2017) Efek Antibakteri Ekstrak Metanol Umbi Tanaman Sarang Semut (Myrmecodia Pendans) Terhadap Streptococcus Mutans Secara In Vitro. Sarjana thesis, Universitas Brawijaya.
Abstract
Bakteri Streptococcus mutansmerupakan bakteri Gram positif yang memiliki proporsi jumlah yang besar pada rongga mulut. Bakteri ini dapat menimbulkan suatu infeksi yang menyebabkan terjadinya perubahan kesehatan pada rongga mulut, seperti plak, gingivitis, denture stomatitits, karies, dan endokarditis. Tanaman sarang semut (Myrmecodia pendans) memiliki kandungan aktif yang diduga bermanfaat sebagai antibakteri seperti flavonoid, tanin, polifenol, terpenoid, tokoferol. Penelitian ini bertujuan untuk membuktikan efek antibakteri ekstrak metanol tanaman sarang semut (Myrmecodia pendans) terhadap bakteri Streptococcus mutans secara in vitro. Penelitian ini merupakan penelitian eksperimental laboratorik dengan menggunakan metode dilusi tabung untuk menentukan Kadar Hambat Minimum (KHM), atau Kadar Bunuh Minimum (KBM). Konsentrasi ekstrak yang digunakan pada penelitian ini yaitu 2%; 2,5%; 3%; 3,5%; 4%; 5%(v/v)dengan tiga kali pengulangan dan Klorheksidin glukonat 0,2% yang digunakan sebagai kontrol pembanding. Konsentrasi bakteri Streptococcus mutans adalah 106CFU/ml. Analisis statistik menunjukkan bahwa ada perbedaan pertumbuhan koloni pada dosis yang berbeda (ANOVA, p= 0,000).Ada hubungan antara dosis ekstrak dengan pertumbuhan koloni (R= -0,888, p=0,00).Dari hasil penelitian didapatkan KHM pada konsentrasi 3,5% v/v dan KBM pada konsentrasi 5%v/v. Dengan demikian dapat disimpulkan bahwa ekstrak metanol tanaman sarang semut (Myrmecodia pendans) memiliki efek antibakteri terhadap Streptococcus mutans secara in vitro.
English Abstract
Streptococcus mutansbacteria is a Gram-positive bacteria with high proportion amount in human cavity. These bacteria can cause infection that affects the health of our cavity, such as plaque, gingivitis, denture stomatitis, caries, and endocarditis. Ant plants (Myrmecodia pendans) have active ingredient that assumed to be effective anti-bacterial compounds such as flavonoid, tannin, polyphenol, terpenoid, tocopherol. The objective of this research is to prove the effect of anti-bacterial effect in the extract of methanol from ant plants (Myrmecodia pendans) towards Streptococcus mutants bacteria in In Vitro process. It is an experimental laboratory research with the method of tube dilution in order to determine the minimum inhibitory concentration (MIC), or the minimum bactericidal concentration (MBC). The concentrations of extract implemented in this research are 2%; 2.5%; 3%; 3.5%; 4%; 5% (v/v) with three times repetition and 0.2% chlorheksidin glukonat as the control variable. The concentration of Streptococcus mutants bacteria is 106CFU/ml.The analysis result showed that there are different colony growth with different given doses of extract (ANOVA, p=0,00). Colony growth are associated with the dose of the extract (R=0,888, p=0,00).From the study, the minimum MIC acquired is at the concentration of 3.5% v/v and MBC at the concentration of 5% v/v. Hence, it can be concluded that the extract of methanol from ant plants (Myrmecodia pendans) have anti-bacterial effect towards Streptococcus mutants bacteria in In Vitro process.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FKG/2017/84/051711782 |
| Uncontrolled Keywords: | Tanaman Sarang Semut (Myrmecodia pendans), antibakteri Streptococcus mutans, ant plants (Myrmecodia pendans), Streptococcus mutants anti-bacterial |
| Subjects: | 600 Technology (Applied sciences) > 615 Pharmacology and therapeutics > 615.3 Organics drugs > 615.32 Drugs derived from plants and mikroorganisms > 615.323 96 Drugs derived from spesific plant (Lamiales) |
| Divisions: | Fakultas Kedokteran Gigi > Kedokteran Gigi |
| Depositing User: | Nur Cholis |
| Date Deposited: | 20 Dec 2017 08:01 |
| Last Modified: | 23 Sep 2020 08:13 |
| URI: | http://repository.ub.ac.id/id/eprint/7312 |
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