Yanti, Christina Sely Fajar (2017) Terapi Ekstrak Etanol Daun Kersen (Muntingia calabura) Terhadap Ekspresi TNF-α (Tumor NecrosisFactor-α) dan Gambaran Histopatologi Hepar Tikus (Rattus norvegicus) Fibrosis Hepar Pasca Induksi CCl4. Sarjana thesis, Universitas Brawijaya.
Abstract
Fibrosis hepar merupakan penyakit hati kronik yang disebabkan akumulasi protein ECM (Ekstraceluller Matrix). Daun kersen (Muntingia calabura) dapat digunakan sebagai antioksidan, antibakteri dan antiinflamasi. Daun kersen mengandung flavonoid yang mampu menghambat efek toksik melalui pengikatan radikal bebas dan dekomposisi peroksida lipid. Penelitian ini mempelajari pengaruh pemberian terapi ekstrak etanol daun kersen (Muntingia Calabura) terhadap ekspresi Tumor Necrosis Factor α dengan metode Imunohistokimia dan melihat gambaran histopatologi hepar model tikus (Rattus norvegicus) fibrosis hepar dengan metode pewarnaan HE. Hewan coba tikus putih (Rattus norvegicus) dibagi menjadi empat kelompok yaitu kontrol, fibrosis hepar, fibrosis hepar yang diberi paparan karbon tetraklorida (CCl4) dengan dosis 0,3 mL/150 g BB secara intaperitoneal (3 kali seminggu selama 4 minggu) selanjutnya diterapi dengan ekstrak etanol daun kersen dengan dosis 100 mg/150 g BB selama 14 hari, fibrosis hepar yang diberi CCl4 selanjutnya diterapi dengan ekstrak etanol daun kersen dengan dosis 300 mg/150 g BB selama 14 hari. Data hasil pengamatan ekspresi TNF- α dan histopatologi hepar dianalisis dengan uji ANOVA dan dilakuan analisis lebih lanjut dengan uji BNJ (α = 0.05 %) untuk mengetahui perbedaan antar kelompok perlakuan dengan terapi yang diberikan. Hasil penelitian menunjukkan bahwa pemberian terapi ekstrak etanol daun kersen (Muntingia calabura) secara signifikan menurunkan kerusakan pada gambaran histopatologi jaringan hepar dan ekspresi TNF- α hepar sebesar 62,21 % dengan dosis terbaik 300 mg/150 g BB. Kesimpulan dari penelitian ini menunjukkan bahwa ekstrak etanol daun kersen dapat digunakan sebagai terapi fibrosis hepar.
English Abstract
Liver fibrosis is a chronic liver disease caused by the accumulation of ECM proteins (Ekstraceluller Matrix). Leaves of cherry (Muntingia calabura) can be used as an antioxidant, antibacterial and anti-inflammatory. Leaves of cherry contains flavonoids that can inhibit the toxic effects by binding free radicals and lipid peroxide decomposition.This research studied the effect of ethanol extract of leaves of cherry therapy (Muntingia calabura) against Tumor Necrosis Factor α expression by Immunohistochemistry method and see the picture of the liver histopathology liver model of rat (Rattus norvegicus) hepatic fibrosis by HE staining method. Rats (Rattus norvegicus) were divided into four groups: control, liver fibrosis, liver fibrosis by exposure to carbon tetrachloride (CCl4) at a dose of 0.3 mL / 150 g BW intaperitoneal (3 times a week for 4 weeks) subsequently treated with ethanolic extract leaves of cherry with a dose of 100 mg/150 g BW for 14 days, liver fibrosis by CCl4 subsequently treated with ethanolic extract leaves of cherry with a dose of 300 mg/150 g BW for 14 days. The data were analyzed with the expression TNF- α and histophatology liver of the ANOVA test and further analysis was done with BNJ test (α = 0.05%) to determine the differences between the group treated with therapy given. Result showed that ethanol extract of cherry leaves (Muntingia calabura) therapy was significantly damage lower of histopathologic features of the liver and TNF- α expression of liver respectively 62,21 % at the best dose of 300 mg/150 grBW. The conclusion of this study showed that the ethanol extract of cherry leaves can be used for the treatment of hepatic fibrosis.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FKH/2017/74/051704886 |
| Uncontrolled Keywords: | Daun Kersen, Fibrosis Hepar, Histopatologi Hepar, Tumor Necrosis Factor α |
| Subjects: | 600 Technology (Applied sciences) > 615 Pharmacology and therapeutics > 615.3 Organics drugs > 615.32 Drugs derived from plants and mikroorganisms > 615.323 674 Drugs derived from specific plants (sapotaceae) > 615.323 68 Drugs derived from specific plants (elaeocarpaceae) |
| Divisions: | Fakultas Kedokteran Hewan > Kedokteran Hewan |
| Depositing User: | Yusuf Dwi N. |
| Date Deposited: | 20 Jul 2017 07:03 |
| Last Modified: | 25 Nov 2020 04:27 |
| URI: | http://repository.ub.ac.id/id/eprint/439 |
Preview |
Text
CHRISTINA SELY FAJAR YANTI.pdf Download (1MB) | Preview |
Actions (login required)
 |
View Item |