BKG

Rahmawati, Eny (2017) Studi Terapi Antiosianin Ubi Jalar Ungu Pada Tikus Rattus Norvegicus Model Stroke Iskemik Terhadap Ekspresi Beta Amyloid, Vegf Dan Kadar Bdnf Pada Cerebellum. Magister thesis, Universitas Brawijaya.

Indonesian Abstract

Stroke iskemik merupakan merupakan penyebab kematian tertinggi ketiga di dunia yang mengakibatkan disabilitas jangka panjang pada penderitanya. Secara umum stroke iskemik disebabkan oleh terhentinya aliran darah menuju otak yang membawa oksigen dan glukosa. Gangguan yang ditimbulkan berakibat pada perubahan seluler sel seperti gangguan homoetasis ion, eksitoksisitas dan kematian sel. Namun, otak juga memiliki kemampuan untuk mempertahankan kelangsungan hidup sel dengan meregulasi faktor neurotropik dan fakor pertumbuhan. Pada penelitian ini digunakan antosianin ubi jalar ungu yang telah dipasarkan secara bebas. Antosianin yang berasal dari ubi jalar ungu diharapkan mampu menekan pembentukan radikal bebas yang ditimbulkan akibat stres oksidatif dan mampu memodulasi sinyal transduksi untuk mengaktifkan pembentukan neurotropin dan faktor pertumbuhan seperti VEGF dan BDNF. Tujuan penelitian ini adalah untuk mengetahui pengaruh pemberian antosianin ubi jalar ungu pada tikus Rattus norvegicus yang mengalami stroke iskemik. Pengamatan didasarkan pada ekspresi Beta Amyloid dan VEGF serta kadar BDNF. Teknik pembuatan model stroke iskemik dilakukan menggunakan teknik MCAO yang dilakukan dengan mengikat arteri CCA dan ECA selama 3 jam yang dilanjutkan dengan reperfusi. Hewan uji dibagi menjadi lima kelompok yang terdiri dari kelompok kontrol (KN), kelompok stroke iskemik pasca 1 jam iskemik reperfusi (K1), kelompok stroke iskemik pasca 72 jam iskemik reperfusi (K2), kelompok terapi 24 jam (T1) dan kelompok terapi 72 jam (T2). Ekspresi Beta Amyloid dan VEGF dianalisis menggunakan teknik immunohistokimia (IHK) dan pengukuran kadar BDNF dilakukan menggunakan uji ELISA. Persentase ekspresi Beta Amyloid pada otak kecil tikus model stroke iskemik kelompok KN, K1, K2, T1 dan T2 berturut-turut sebesar 16,92% ± 2,17; 32,82% ± 3,66; 35,78% ± 1,83; 20,68% ± 1,32; 17,0% ± 1,57. Terapi antosianin menunjukkan penurunan ekspresi Beta Amyloid yang sangat signifikan pada kelompok T1 dan T2 yaitu berturut-turut sebesar 36,99% dan 48,20% yang sebelumnya mengalami peningkatan sebesar 111,47% pada K2. Sementara itu, Hasil IHK ekspresi VEGF menunjukkan persentase berturut-turut sebesar 26,6% ± 1,6; 43,9% ± 2,6; 44,8% ± 2,7; 70,2% ± 2,6; 53,5% ± 3,6. Berdasarkan hasil IHK yang diperoleh, secara alami otak kecil mengekspresikan VEGF terutama pada 1 jam iskemik reperfusi yang dibuktikan dengan kenaikan ekspresi VEGF sebesar 65,04% namun pemberian ekstrak antosianin mampu membantu peningkatan VEGF yang cukup signifikan pada kelompok terapi T1 dan T2. Pemeriksaan kadar BDNF pada kelompok perlakukan berturut-turut sebesar 402,04 (pg/mg) ± 31,63; 306,36 (pg/mg) ± 41,01; 526,59 (pg/mg) ± 43,93; 881,81 (pg/mg) ± 51,76 dan 1029,54 (pg/mg) ± 5,58 (pg/mg). Berdasarkan kadar BDNF yang diperoleh, pemberian antosianin ubi jalar ungu memperlihatkan peningkatan kadar BDNF yang cukup signifikan. Berdasarkan keseluruhan data yang diperoleh dapat disimpulkan bahwa pemberian antosianin ubi jalar ungu dapat digunakan untuk terapi stroke iskemik yang didasarkan pada penurunan ekspresi Beta Amyloid dan peningkatan ekspresi VEGF dan kadar BDNF

English Abstract

Ischemic stroke is the third leading cause of death in the world resulting in long-term disability in the sufferer. Ischemic stroke is caused by blocking of blood flow to the brain carrying oxygen and glucose. The disruption results in cellular changes in brain cells such as ion homeostasis, excitotoxicity, and cell death. However, the brain also has the ability to maintain cell survival by regulating neurotrophic and growth factors. In this study, we used anthocyanin from purple sweet potato that has been marketed. Anthocyanin from purple sweet potatoes is expected to suppress free radicals caused by oxidative stress and able to modulate the signal transduction to activate the cell defense system such as enhancing the neurotrophic and growth factors. The aim of this study was to determine the effect of anthocyanin from purple sweet potato in ischemic stroke rats based on Beta Amyloid and VEGF expression and BDNF levels in the cerebellum. We used MCAO technique to make ischemic stroke rats by ligating CCA and ECA for 3 h followed by reperfusion. Rats were divided into five groups consisting of the control group (KN), ischemic stroke group after 1 h (K1), ischemic stroke group after 72 h (K2), therapy group of 24 h (T1) and therapy group oh 72 h (T2). The expression of Beta Amyloid and VEGF were analyzed using immunohistochemistry (IHC) and BDNF levels determined by ELISA assays. The percentage of Beta Amyloid expression in the cerebellum of ischemic stroke rats of KN, K1, K2, T1, and T2 were respectively 16.92% ± 2.17; 32.82% ± 3.66; 35.78% ± 1.83; 20.68% ± 1.32; 17% ± 1.57. Therapy of anthocyanin showed a decrease in Amyloid Beta expression significantly in the T1 and T2 groups of 36.99% and 48.20%, respectively, which was previously exposed to 111.47% in K2. The percentage of VEGF expression in groups respectively of 26.6% ± 1.6; 43.9% ± 2.6; 44.8% ± 2.7; 70.2% ± 2.6; 53.5% ± 3.6. Based on the IHC results, naturally the cerebellum expresses VEGF at 1 h after reperfusion ischemia as evidenced by an increase in VEGF expression of 65.04% but the anthocyanin from purple sweet was able to assist in a t increase significant VEGF in the T1 and T2 therapy groups. The measurement of BDNF levels in the group respectively were 402.04 (pg/mg). ± 31,63; 306.36 (pg/mg) ± 41.01; 526,59 (pg/mg) ± 43,93; 881.81 (pg/mg) ± 51.76 and 1029.54 (pg/mg) ± 5.58 (pg/mg). Based on the levels of BDNF, anthocyanin from purple sweet potato increases BDNF levels significantly. It can be concluded that anthocyanin from purple sweet potato can be used as a therapeutic agent in brain ischemic stroke rats.

Other Language Abstract

UNSPECIFIED

Item Type: Thesis (Magister)
Identification Number: TES/615.323 94/RAH/s/2017/041709011
Uncontrolled Keywords: IPOMOEA, ANTHOCYANIN, CEREBROVASCULAR DISEASE, CEREBROVASCULAR - DISEASE DIET THERAPY, AMYLOID BETA - PROTEIN, VASCULAR ENDOTHELIAL GROWTH FACTOR, CEREBELLUM
Subjects: 500 Natural sciences and mathematics > 571 Physiology and related subjects > 571.4 Biophysics
Divisions: S2 / S3 > Magister Kimia, Fakultas MIPA
Depositing User: Nur Cholis
URI: http://repository.ub.ac.id/id/eprint/3033
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