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Kaliappan, Gayathiri A/P (2019) The Effects of Pomegranate (Punica granatum) Peel Extract as Antibacterial Agent Against Staphylococcus aureus In Vitro. Sarjana thesis, Universitas Brawijaya.

Indonesian Abstract

Staphylococcus aureus adalah penyebab penting infeksi nosokomial dan didapat di masyarakat. Selain itu, penting untuk menentukan isolat yang relevan yang diperoleh selama percobaan. Jadi, itu juga salah satu organisme yang sering ditemukan pada infeksi manusia. S. aureus adalah kasus utama di rumah sakit. Senyawa aktif dalam kulit delima (Punica granatum) mengandung flavonoid, tannis, pyrogallol, asam sinamat, asam benzoat, asam klorogenat, campferol, genistein, asam coumaric, dan quercetin yang membantu antibiotik melawan Staphylococcus aureus. Tujuan dari penelitian ini adalah untuk membuktikan efek antimikroba dari ekstrak kulit buah delima terhadap pertumbuhan bakteri Staphylococcus aureus secara in vitro menggunakan metode difusi agar. Ekstrak kulit delima dengan konsentrasi 3%, 6%, 9%, 12%, 15%, 18% dan 21% digunakan dalam tiga isolat Staphylococcus aureus yang berbeda. Antibiotik seperti Gentamicin dan Fosfomycin digunakan dalam percobaan ini. Dengan demikian, untuk menentukan efek antimikroba dari ekstrak kulit buah delima (Punica granatum) diukur dengan zona hambatan yang terbentuk di sekitar sumur dalam percobaan ini. Selanjutnya untuk analisis data, ANOVA satu arah digunakan dalam penelitian ini dan diverifikasi bahwa konsentrasi ekstrak kulit buah delima dan pertumbuhan S. aureus adalah p <0,05. Selain itu, untuk memperkuat analisis data di atas, hasil korelasi Spearmen juga menunjukkan korelasi yang dominan antara konsentrasi ekstrak dan penghambatan zona dengan menggambarkan nilai p <005 dan r = 0,843. Kesimpulannya, hasil penelitian membuktikan bahwa ekstrak kulit buah delima memiliki efek antimikroba terhadap Staphylococcus aureus secara in vitro menggunakan metode difusi sumur.

English Abstract

Staphylococcus aureus is an important cause of nosocomial infections and is acquired in the community. In addition, it is important to determine the relevant isolates obtained during the experiment. So, it is also one of the organisms that is often found in human infections. S. aureus is a major case in hospital. The active compound in pomegranate skin (Punica granatum) contains flavonoids, tannis, pyrogallol, cinnamic acid, benzoic acid, chlorogenic acid, campferol, genistein, coumaric acid, and quercetin which help antibiotics against Staphylococcus aureus. The purpose of this study is to prove the antimicrobial effect of pomegranate skin extract on the growth of Staphylococcus aureus bacteria in vitro using agar diffusion method. Pomegranate extract with concentrations of 3%, 6%, 9%, 12%, 15%, 18% and 21% was used in three different isolates of Staphylococcus aureus. Antibiotics such as Gentamicin and Fosfomycin were used in this experiment. Thus, to determine the antimicrobial effect of pomegranate peel extract (Punica granatum) was measured by the zone of inhibition formed around the well in this experiment. Furthermore, for data analysis, one-way ANOVA was used in this study and it was verified that the concentration of pomegranate rind extract and growth of S. aureus was p <0.05. In addition, to strengthen the analysis of the data above, the Spearmen correlation results also showed a dominant correlation between extract concentration and zone inhibition by describing p values <005 and r = 0.843. In conclusion, the results of the study prove that pomegranate skin extract has an antimicrobial effect against Staphylococcus aureus in vitro using the well diffusion method.

Other Language Abstract

UNSPECIFIED

Item Type: Thesis (Sarjana)
Identification Number: SKR/FK/2019/615/052001470
Uncontrolled Keywords: Antibacterial, Staphylococcus aureus ;Pomegranate peel,Well diffusion-Antibacterial; Staphylococcus aureus; Pomegranate peel; Well diffusion.
Subjects: 600 Technology (Applied sciences) > 615 Pharmacology and therapeutics > 615.3 Organics drugs > 615.32 Drugs derived from plants and mikroorganisms
Divisions: Fakultas Kedokteran > Pendidikan Dokter
Depositing User: soegeng
URI: http://repository.ub.ac.id/id/eprint/180430
Text
Gayathiri AP Kaliappan.pdf

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