BKG

Tjandra, Bartolomeus Alvin (2019) Pengaruh Ekstraks Seledri (Apium graveolens) Terhadap Pertumbuhan Bakteri Shigella dysentriae secara In Vitro. Sarjana thesis, Universitas Brawijaya.

Indonesian Abstract

Shigella dysentriae salah satu organisme yang dapat meyebabkan penyakit disentri dimana manifestasi klinis yang muncul dapat menyebabkan gejala berupa diare berair yang disertai dehidrasi hingga nyeri perut dengan tinja mucoid yang disertai darah. Infeksi Shigella dysentriae pada anak dapat menyebabkan manifestasi klinis yang lebih berat berupa nyeri kepala, meningismus, kejang, arthritis dan hemolytic uremic syndrome. Secara epidemiologis, infeksi Shigella dysentriae masih sering dijumpai di negara berkembang dimana tingkat sanitasi lingkungan masih rendah dan praktek defekasi terbuka masih berlangsung. Dalam beberapa dekade terakhir, resistensi antibiotik terapi konvensional untuk terapi infeksi Shigella dysentriae mulai bermunculan sehingga diperlukan pencarian senyawa yang efektif untuk pengobatan infeksi Shigella dysentriae. Penelitian ini ditujukan untuk menggali efektivitas eksrak etanol seledri terhadap pertumbuhan bakteri Shigella dysentriae secara in-vitro. Sampel diperoleh dari isolat bakteri yang disediakan di Laboratorium Mikrobiologi FKUB. Konsentrasi ekstrak yang dipakai terdiri dari konsentrasi 0%, 22%, 23%, 24%, 25%, 26% dan 27%. Metode yang digunakan untuk menguji efektivitas ekstrak seledri adalah metode dilusi tabung dengan kadar bunuh minimum pada konsentrasi 27% dan kadar hambat minimum pada konsentrasi 23% Berdasarkan uji one way ANOVA, terdapat perbedaan signifikan jika ada perubahan konsentrasi ekstrak seledri terhadap pertumbuhan koloni Shigella dysentriae (Korelasi, r=-0,877: p<0,05) dan terbukti bahwa ekstrak seledri memiliki efek antimikroba terhadap bakteri Shigella dysentriae.

English Abstract

Shigella dysentriae is one of the organism that is capable of causing dysentery which symptoms usually varies between watery diarrhea and abdominal pain to mucoid stool that are often accompanied by blood. Shigella dysentriae infection in pediatric patients often leads to heavier clinical manifestation that consists of headache, meningism, seizure, arthritis, and hemolytic uremic syndrome. Epidemiologically, Shigella dysentriae infection can be often found on developing nations where sanitation standards are poor and the practice of open defecation is still prevalent. Antibiotic resistance of Shigella dysentriae against conventional medication has been reported which requires other substances that are capable of treating Shigella dysentriae infection. Through this research, the effectiveness of celery as an antimicrobial substance against Shigella dysentriae in vitro will be explored. Shigella dysentriae specimen was acquired from Brawijaya University School of Medicine Micbrobiology Laboratory. The concentration levels are 0%, 22%, 23%, 24%, 25%, 26%, and 27%. The method used in this research to test the effect of the extract is tube dilution resulted in a 27% Minimum Bactericidal Concentration and 23% Minimum Inhibitory Concentration.Based on oneway ANOVA test, a significant correlation between extract concentration alterations and the number of Shigella dysentriae colony that was growing on the plate can be found (Correlation, r=-0,877: p<0,05).

Other Language Abstract

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Item Type: Thesis (Sarjana)
Identification Number: SKR/FK/2019/449/052001273
Uncontrolled Keywords: Shigella dysentriae, ekstrak etanol seledri (Apium graveolens), antibakteri, Shigella dysentriae, celery (Apium graveolens) ethanol extract, antimicrobial
Subjects: 600 Technology (Applied sciences) > 615 Pharmacology and therapeutics > 615.3 Organics drugs > 615.32 Drugs derived from plants and mikroorganisms
Divisions: Fakultas Kedokteran > Pendidikan Dokter
Depositing User: Budi Wahyono
URI: http://repository.ub.ac.id/id/eprint/180122
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