Naufal, Muhammad Taufiqul (2019) Kloning Gen Penyandi Xilanase GH11 dari Bacillus halodurans CM1 ke Escherichia coli DH5α. Sarjana thesis, Universitas Brawijaya.
Abstract
Xilanase merupakan enzim yang berfungsi untuk memecah xilan menjadi xilosa dan enzim ini sudah digunakan pada berbagai industri. Melihat banyaknya aplikasi dari enzim ini, peneliti banyak melakukan penelitian mengenai bagaimana cara meningkatkan produktivitas dan efektifitas dari enzim xilanase. Salah satu cara melakukan peningkatan dalam proses produksi enzim xilanase adalah dengan menggunakan teknologi DNA yaitu kloning. Bacillus halodurans CM1 merupakan bakteri alkalotermofilik yang dapat dijadikan sumber gen penyandi xilanase yang potensial. Penelitian ini dilakukan untuk melakukan kloning gen penyandi xilanase GH11 yang berasal dari Bacillus halodurans CM1 menggunakan plasmid pJET 1.2/blunt sebagai vektor dengan Escherichia coli DH5α sebagai sel inang dan mengetahui urutan basa nukleotida gen penyandi xilanase GH11 yang berasal dari Bacillus halodurans CM1. Hasil penelitian menunjukan gen xilanase GH11 yang berasal dari Bacillus halodurans CM1 berhasil dikloning ke dalam plasmid pJET dengan host Esherichia coli DH5α dan berdasarkan hasil BLAST nukleotida dan protein dari hasil sekuensing dapat dilihat jika terdapat kemiripan pada gen dan protein dengan endo-1,4-betaxylanhydrolase (xyn11A) dari Bacillus halodurans C-125 sebesar 99%.
English Abstract
Enzymes are biocatalysts which have been widely used in various fields such as in industry. One enzyme that is widely used in industry is xylanase. Xylanase is an enzyme that can break down xylan into xylose and xylooligosaccharide. Seeing the many applications of this enzyme, researchers conducted many studies on how to increase the productivity and effectiveness of the xylanase enzyme. One of the method that can be used to increase the xylanase enzyme production process is by using recombinant DNA technology. One of the method in recombinant technology is cloning. Bacillus halodurans CM1 is an alkalothermophilic bacterium that can be a potential source of xylanase coding genes for cloning. This research was conducted to clone the GH11 xylanase coding gene from Bacillus halodurans CM1 using pJET 1.2 / blunt plasmid as vector with Escherichia coli DH5α as cell host and determine the nucleotide base sequence of the GH11 xylanase coding gene from Bacillus halodurans CM1. The results showed the GH11 xylanase gene from Bacillus halodurans CM1 was successfully cloned into a pJET plasmid as vector with Esherichia coli DH5α as cell host and based on the results of BLAST nucleotides and proteins of the sequencing results can be seen if there are similarities in genes and proteins with endo-1,4-beta -xylanhydrolase (xyn11A) from Bacillus halodurans C-125 by 99%.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FTP/2019/545/052002671 |
| Uncontrolled Keywords: | Kloning, xilanase, Bacillus halodurans CM1.- Cloning, xylanase, Bacillus halodurans CM1. |
| Subjects: | 500 Natural sciences and mathematics > 579 Natural history of microorganisms, fungi, algae > 579.3 Prokaryotes > > 579.362 Bacillus |
| Divisions: | Fakultas Teknologi Pertanian > Teknologi Hasil Pertanian |
| Depositing User: | Agus Wicaksono |
| Date Deposited: | 18 Aug 2020 03:14 |
| Last Modified: | 18 Aug 2020 03:14 |
| URI: | http://repository.ub.ac.id/id/eprint/180089 |
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