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Wiranata, Jones Putra (2019) Efek Ekstrak Etanol Seledri (Apium Graveolens) Sebagai Penghambat Pertumbuhan Bakteri Methicillin-Resistant Staphylococcus Aureus Secara In Vitro. Sarjana thesis, Universitas Brawijaya.

Indonesian Abstract

Staphylococcus aureus merupakan salah satu bakteri yang paling sering menyebabkan infeksi. Sifat adaptif Staphylococcus aureus terhadap antibiotik menyebabkan lahirnya strain baru yang dikenal sebagai Methicillin-Resistant Staphylococcus aureus (MRSA). Berdasarkan alasan tersebut, perlu dikembangkan obat alternatif yang dapat melawan infeksi bakteri MRSA. Salah satu alternatifnya yaitu dengan menggunakan ekstrak tanaman sudah banyak diteliti khasiatnya, yaitu seledri (Apium graveolens). Seledri mengandung berbagai senyawa kimia yang berpotensi sebagai antimikroba, seperti, flavonoid, tannin, limonen, dan asam klorogenat. Penelitian ini bertujuan untuk membuktikan efek antimikroba seledri (Apium graveolens) terhadap bakteri MRSA dengan metode dilusi tabung. Konsentrasi ekstrak seledri (Apium graveolens) yang digunakan dalam penelitian adalah 27%, 29%, 31%, 33%, 35%, dan 37% dengan 4 kali pengulangan. Pengamatan Kadar Hambat Minimal (KHM) secara kualitatif didapatkan pada konsentrasi 27%. Perhitungan Kadar Bunuh Minimal (KBM) secara kuantitatif didapatkan pada konsentrasi 37%. Analisa statistik menggunakan One-Way ANOVA menunjukkan perbedaan yang signifikan pada setiap perubahan konsentrasi ekstrak seledri (Apium graveolens) terhadap pertumbuhan koloni MRSA (r=-0,982; p<0,05). Berdasarkan penelitian ini, dapat disimpulkan bahwa ekstrak etanol seledri (Apium graveolens) dapat menghambat pertumbuhan MRSA secara in vitro.

English Abstract

Staphylococcus aureus is one of the most common causes of clinical infections. Adaptive nature of Staphylococcus aureus to antibiotics induced the emergence of new strain known as Methicillin-Resistant Staphylococcus aureus (MRSA). Based on these reasons, it is necessary to develop alternative drugs that can fight MRSA bacterial infections. One alternative is to use plant extracts that have many studied properties, namely celery (Apium graveolens). Celery contains various chemical compounds that have the potential as antimicrobial agents, such as flavonoids, tannins, limonene, and chlorogenic acid. This study aims to prove the antimicrobial effect of celery (Apium graveolens) on MRSA bacteria using the method of tube dilution. The concentration of celery extract (Apium graveolens) used in the study was 27%, 29%, 31%, 33%, 35%, and 37% with 4-time iterations. Observation of Minimal Inhibitory Concentration (MIC) qualitatively was recorded at a concentration of 27%. Minimum Bactericidal Concentration (MBC) is obtained at a concentration of 37%. Statistical analysis using One-Way ANOVA showed significant differences in each alteration of concentration of celery extract (Apium graveolens) with respect to the growth of MRSA colonies (r = -0.982; p <0.05). Based on this research, it was concluded that the ethanol extract from celery (Apium graveolens) could restrain the growth of MRSA through in vitro process.

Other Language Abstract

UNSPECIFIED

Item Type: Thesis (Sarjana)
Identification Number: SKR/FK/2019/453/052001277
Uncontrolled Keywords: MRSA, seledri, KHM, KBM
Subjects: 600 Technology (Applied sciences) > 615 Pharmacology and therapeutics > 615.3 Organics drugs > 615.32 Drugs derived from plants and mikroorganisms
Divisions: Fakultas Kedokteran > Pendidikan Dokter
Depositing User: soegeng
URI: http://repository.ub.ac.id/id/eprint/179853
Text
Jones Putra Wiranata.pdf

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