BKG

Maulidina, Ade Ika (2019) Penentuan Kondisi Optimum Amobilisasi Xilanase dari Aspergillus niger pada Matriks Pasir Laut. Sarjana thesis, Universitas Brawijaya.

Indonesian Abstract

Amobilisasi enzim dilakukan untuk mencegah kerusakan enzim akibat pengaruh lingkungan dan untuk mempertahankan stabilitas enzim, karena enzim bebas tidak stabil terhadap pengaruh lingkungan. Enzim xilanase yang digunakan diisolasi dari jamur Aspergillus niger. Amobilisasi xilanase dilakukan pada matriks pasir laut menggunakan metode adsorpsi. Penelitian ini bertujuan untuk mengetahui waktu pengocokan dan konsentrasi enzim optimum serta efisiensi penggunaan berulang xilanase amobil. Enzim xilanase yang digunakan untuk amobilisasi memiliki konsentrasi sebesar 1,475 mg/mL dengan aktivitas enzim 21,674 Unit. Variasi waktu pengocokan yang dilakukan adalah (1,2,3,4, dan 5) jam. Variasi konsentrasi enzim yang digunakan adalah (0,147; 0,442; 0,737; 0,997; dan 1,282) mg/mL. Kondisi optimum amobilisasi ditentukan dengan mengukur aktivitas enzim dan jumlah enzim yang teradsorpsi pada enzim amobil. Pada penelitian ini didapatkan kondisi optimum amobilisasi xilanase adalah pada waktu pengocokan 2 jam dan konsentrasi enzim 0,997 mg/mL dengan massa enzim teradsorpsi pada pasir laut sebesar 2,904 mg dan aktivitas enzim amobil 6,487 Unit. Efisiensi penggunaan berulang xilanase amobil pada matriks pasir laut didapatkan sebanyak 3 kali dengan memberikan aktivitas enzim sisa 60,47 %.

English Abstract

Enzyme immobilization is carried out to prevent enzyme damage due to environmental influences and to maintain stabilization of enzyme, because free enzyme is not stable to environment changes. The xylanase was isolated from Aspergillus niger. Immobilization of xylanase was carried out on the sea sand matrix using the adsorption method. This study aims to determine optimum shaking time and concentration of enzyme, and efficiency of repeated use of immobilized xylanase. The xylanase used for immobilization has concentration of 1.475 mg/mL with enzyme activity was 21.674 Units. Shaking time variation were (1,2,3,4 and 5) hours. Concentration of enzyme variation were (0.147; 0.442; 0.737; 0.997; and 1,282) mg/mL. Optimum conditions for immobilization are determined by measuring enzyme activity and the amount of enzymes adsorbed on immobilized enzymes. In this study the optimum conditions for xylanase immobilization was obtained at 2 hours shaking time and concentration of enzyme 0.997 mg/mL with an enzyme mass adsorbed on sea sand was 2.904 mg and activity of immobilized enzyme 6.487 Units. The efficiency of repeated use of immobilized xylanase in sea sand matrix was 3 times with enzyme activity still at 60.47%.

Other Language Abstract

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Item Type: Thesis (Sarjana)
Identification Number: SKR/MIPA/2019/318/051910989
Uncontrolled Keywords: Amobilisasi, Xilanase, Aspergillus niger, Adsorpsi, Pasir Laut, Immobilization, Xylanase, Aspergillus niger, Adsorption, Sea Sand
Subjects: 500 Natural sciences and mathematics > 512 Algebra > 512.9 Foundation of algebra > 512.943 4 Matrices
Divisions: Fakultas Matematika dan Ilmu Pengetahuan Alam > Kimia
Depositing User: Budi Wahyono
URI: http://repository.ub.ac.id/id/eprint/179450
Text
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