Kardikadewi, Varellia Awang Wangi (2019) Pengaruh Gelatin Ikan Patin (Pangasius djambal) terhadap ekspresi Epidermal Growth Factor pada luka pasca pencabutan gigi tikus putih (Rattus norvegicus). Sarjana thesis, Universitas Brawijaya.
Abstract
Proses pencabutan gigi sering menimbulkan luka baik di jaringan lunak maupun jaringan keras. Saat terjadi luka, beberapa growth factor dilepaskan bersamaan dengan mediator inflamasi lainnya salah satunya adalah EGF Gelatin ikan patin (Pangasius djambal) diketahui memiliki banyak kandungan asam amino glisin yang berperan dalam proses penyembuhan luka. Penelitian ini dilakukan dengan tujuan untuk mengetahui pengaruh pemberian gelatin ikan patin (Pangasius djambal) terhadap jumlah ekspresi EGF pasca pencabutan gigi tikus putih (Rattus norvegicus). Pada penelitian ini terdapat 6 kelompok sampel, terdiri dari kelompok kontrol (K) dan kelompok perlakuan (P) pada hari ketiga, kelima, dan ketujuh pasca dilakukannya ekstraksi gigi. Sampel dilakukan pewarnaan menggunaan metode imunohistokimia dan hasil pengamatan ekspresi EGF diamati dengan menggunakan mikroskop cahaya perbesaran 400x. Setelah ditemukan jumlah rerata ekspresi EGF, kemudian dilakukan uji analisis menggunakan one way ANOVA dan didapatkan perbedaan yang bermakna antara jumlah ekspresi EGF kelompok kontrol dan kelompok perlakuan, dimana nilai ekspresi EGF terendah yaitu pada kelompok kontrol hari ketiga dan nilai ekspresi EGF tertinggi yaitu pada kelompok perlakuan hari ketujuh. Kesimpulan dari penelitian ini adalah pemberian gelatin ikan patin (Pangasius djambal) berpengaruh terhadap peningkatan ekspresi EGF pada luka pasca pencabutan gigi tikus putih (Rattus norvegicus).
English Abstract
A tooth extraction process often causes injury both in soft and hard tissue. When a wound occurs, several growth factors are released together with other inflammatory mediators, one of them is EGF to help the wound healing process. Gelatine is known to have a lot of amino acid glycine that plays an important role in wound healing process. The purpose of this study was to determine the effect of patin fish (Pangasius djambal) gelatin to the number of EGF expression in wound after white rat’s (Rattus norvegicus) tooth extraction. There were six samples groups in this study, consisting of the control groups (K) and the treatment groups (P) on third, fifth and seventh days after the tooth extraction. The samples were stained using immunohistochemical methods and continued with observing the EGF expressions using a light microscope with 400x magnification. After getting the average number of EGF expressions, the next step is doing an analysis using one way ANOVA method and can be found a significant difference between the number of EGF expression in control and treatment groups, there were the lowest EGF expression number was found in the third day of the control group while the highest EGF expression number was found in the seventh day of the treatment group. The conclusion of this study is the patin fish (Pangasius djambal) gelatin has an effect to increase the number of EGF expression in wound after white rat’s (Rattus norvegicus) tooth extraction.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FKG/2019/127/052001889 |
| Uncontrolled Keywords: | EGF, gelatin ikan patin, glisin, pencabutan gigi-EGF, patin fish gelatine, glycine, tooth extraction |
| Subjects: | 600 Technology (Applied sciences) > 617 Surgery, regional medicine, dentistry, ophthalmology, otology, audiology > 617.6 Dentistry > 617.66 Extraction / Teeth--Extraction > 617.660 6 Teeth--Extraction--Treatment |
| Divisions: | Fakultas Kedokteran Gigi > Kedokteran Gigi |
| Depositing User: | soegeng sugeng |
| Date Deposited: | 19 Jul 2020 06:57 |
| Last Modified: | 23 Oct 2021 02:29 |
| URI: | http://repository.ub.ac.id/id/eprint/178903 |
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