Uji Sensitivitas Fusarium Oxysporum F.Sp. Cubense Terhadap Beberapa Bahan Aktif Fungisida Secara In Vitro

Richard, Samuel (2019) Uji Sensitivitas Fusarium Oxysporum F.Sp. Cubense Terhadap Beberapa Bahan Aktif Fungisida Secara In Vitro. Sarjana thesis, Universitas Brawijaya.

Abstract

Pisang merupakan salah satu komoditas pertanian yang sangat popular untuk dikonsumsi oleh masyarakat Indonesia karena rasanya yang lezat pisang dan bergizi. Meskipun memiliki banyak manfat, produksi dari komoditas pisang masih terbilang rendah di Indonesia karena berbagai faktor seperti Hama dan Penyakit. Penyakit layu fusarium yang disebabkan oleh jamur patogen Fusarium oxysporum f. sp.cubense (Foc) merupakan salah satu penyakit tanaman pisang. Dalam praktiknya, pengendalian secara kimiawi dengan menggunaan fungisida sering kali digunakan untuk mengatasi penyakit tersebut di Indonesia namun kurangnya informasi akan bahan aktif dari fungisida yang digunakan membuat pengendalian penyakit tersebut tidak efektif. Penelitian ini bertujuan untuk mengetahui bagaimana respon sensitiv yang diberikan oleh jamur patogen Fusarium oxysporum f.sp Cubense terhadap beberapa jenis fungisida. Penelitian dilaksanakan pada bulan Februari – Juni 2019. Penelitian menggunakan Rancangan Acak Lengkap Faktorial (RALF) dengan dua faktor yaitu 5 jenis bahan aktif fungisida (Azoksistrobin, Asam Fosfit, Propineb, Prokimidon, dan Benomil) dan tiga konsentrasi (500, 1000, 1500 ppm) dengan tiga kali pengulangan serta kontrol. Perlakuan dilakukan dengan peletakan jamur Foc pada media PDA yang tercampur fungisida. Pengamatan yang dilakukan berupa presentase daya hambat fungisida terhadap diameter koloni jamur dan viabilitas konidia. Data yang diperoleh dianalisis menggunakan analisis ragam (Anova) dan Apabila data berbeda nyata maka dilanjutkan uji lanjut Duncan’s Multiple Range Test (DMRT) pada taraf nyata 5%. Hasil penelitian menunjukan bahwa kelima bahan aktif fungisida mampu menghambat pertumbuhan jamur patogen Foc. Faktor bahan aktif memberikan pengaruh yang berbeda nyata terhadap pertumbuhan jamur patogen naamun perbedaan konsentrasi sebesar 500, 1000, dan 1500 ppm tidak memberikan pengaruh berbeda nyata. Pengujian beberapa bahan aktif fungisida yang yang paling efektif menghambat pertumbuhan jamur patogen Foc adalah pada penambahan bahan aktif Benomil. Bahan aktif yang aling efektif dalam menghambat viabilitas konidia adalah pada fungisida Benomil, Propineb, dan Azoksistrobin.

English Abstract

Banana is one the most popular agricultural commodities that been consumed by Indonesian people because of its delicious taste and its nutritious factor. Despite having many benefits, the production of banana commodities is still relatively low in Indonesia due to various factors such as Pests and Diseases. Fusarium wilt caused by Fusarium oxysporum f. sp. cubense (foc) is one of the diseases in banana. In cultivation practice, chemical control using fungicide is often used to treat the disease in Indonesia but the lack of information on the active ingredients of the fungicide used makes the disease control ineffective. This research aims to determine how sensitive responses are given by pathogenic fungi foc against several types of fungicides. The research was conducted in February - June 2019. This research used a Factorial Completely Randomized Design (FCRD) with two factors, which was 5 types of fungicidal active ingredients (Azoxistrobin, Phosphite Acid, Propineb, Procimidone, and Benomyl) and three concentrations (500, 1000, 1500 ppm ) with three repetitions and controls. The treatment is done by placing foc fungi on ADP media mixed with fungicides. Observations were made in the form of a percentage of fungicidal inhibitory capability on the diameter of fungal colonies and conidial viability. The data obtained were analyzed using variance analysis (ANOVA) and if the data were significantly different then continued testing of Duncan 's Multiple Range Test (DMRT) at the 5%. The results showed that the five active ingredients of fungicides were able to inhibit the growth of pathogenic fungi. The active ingredient factors gave a significantly different effect on the growth of pathogenic fungi but the differences in concentrations of 500, 1000, and 1500 ppm did not have a significant effect. The testing of several fungicidal active ingredients which were the most effective in inhibiting the growth of fungal pathogenic fungi was the addition of Benomil active ingredients of 91.11%. The active ingredients which are effective in inhibiting the viability of conidia are Benomyl 1500 ppm fungicides, Propineb 1500 ppm, and Azoxistrobin 1500 ppm at 0%.

Other obstract

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Item Type: Thesis (Sarjana)
Identification Number: SKR/FP/2019/341/051907093
Subjects: 600 Technology (Applied sciences) > 632 Plant injuries, diseases, pests > 632.9 General topics of pest and disease control > 632.95 Pesticides > 632.952 Fungicides
Divisions: Fakultas Pertanian > Hama dan Penyakit Tanaman
Depositing User: Nur Cholis
Date Deposited: 24 Aug 2020 07:11
Last Modified: 22 Oct 2021 06:57
URI: http://repository.ub.ac.id/id/eprint/173335
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