Hanani, Shofiatul (2018) Penentuan Kondisi Optimum Xilanase dari Trichoderma viride yang diamobilisasi pada Matriks Zeolit Ca-Alginat. Sarjana thesis, Universitas Brawijaya.
Abstract
Xilanase merupakan salah satu jenis enzim yang dihasilkan oleh kapang Trichoderma viride yang memiliki kemampuan untuk mengkatalisis reaksi hidrolisis xilan menjadi xilosa. Amobilisasi xilanase bertujuan untuk meningkat ketahanan enzim terhadap perubahan lingkungan yang ekstrem dan agar mampu digunakan berulang kali. Tujuan dari penelitian ini adalah mengetahui kondisi optimum meliputi pH, temperatur, dan waktu inkubasi xilanase yang diamobilisasi pada matriks zeolit Ca-alginat. Kondisi optimum dapat ditentukan dengan cara mengukur aktivitas xilanase pada variasi pH (3, 4, 5, 6, 7), temperatur (20, 30, 40, 50, 60)˚C dan waktu inkubasi (35, 40, 45, 50, 55) menit pada xilanase bebas dan amobil. Diperoleh kondisi optimum xilanase bebas dan amobil masing-masing pada pH 6. Temperatur optimum xilanase bebas dan amobil masing-masing dicapai pada temperatur 50˚C dan 40˚C, serta waktu inkubasi optimum xilanase bebas dan amobil masing-masing dicapai pada 55 dan 45 menit.
English Abstract
Xylanase is one type of enzyme produced by Trichoderma viride mold which has the ability to catalyze the hydrolysis reaction of xylan into xylose. The immobilization of xylanase was used to increase the enzyme's resistance to extreme environmental changes and can be used repeatedly. The purpose of this research is to know the optimum conditions include pH, temperature, and incubation time of immobilized xylanase on zeolite Ca-alginate matrix. The optimum conditions can be determined by measuring xylanase activity with variation of pH (3, 4, 5, 6, 7), temperature (20, 30, 40, 50, 60) ˚C and incubation time (35, 40, 45, 50, 55) minutes on free and immobilized xylanases. The optimum condition of free and immobilized xylanase were reached at pH 6. The optimum temperature of both free and immobilized xylanases were reached at 50˚C and 40˚C, and the optimum incubation time of free and immobilized xylanase were reached 55 and 45 minute.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/MIPA/2018/355/051807408 |
| Uncontrolled Keywords: | Amobilisasi xilanase, Trichoderma viride, zeolit, Ca-alginat, kondisi optimum Immobilized xylanase, Trichoderma viride, Zeolite, Ca-alginate, Optimum condition |
| Subjects: | 500 Natural sciences and mathematics > 579 Natural history of microorganisms, fungi, algae > 579.5 Fungi > 579.56 Ascomycotina ( ascomycetes) > 579.567 Pyrenomycetes > 579.567 7 Hypocreales |
| Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam > Kimia |
| Depositing User: | Budi Wahyono Wahyono |
| Date Deposited: | 23 Jul 2020 06:22 |
| Last Modified: | 18 Oct 2021 03:53 |
| URI: | http://repository.ub.ac.id/id/eprint/168827 |
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