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Gasti,, Detria Rahma (2018) Uji Sitotoksik Kombinasi 5-Fluorourasil dan Coelomic Fluid dari Lumbricus rubellus Terhadap Cell Line HT-29 Secara In Vitro. Sarjana thesis, Universitas Brawijaya.

Indonesian Abstract

Kankerikolorektal merupakan salah satu jenis kanker yang terjadi pada mukosa kolon di mana penyakit ini mempunyai angka morbiditas dan mortalitas yang tinggi. kombinasi 5-fluorourasil dan coelomic fluid dari Lumbricus rubellus diharapkanXmenjadi salah satu alternatif terapi kombinasi yang mampu meningkatkan efek sitotoksik pada cell line HT-29. Uji sitotoksik dilakukan dengan metode uji MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) dengan pemberian konsentrasi kombinasi 5-fluorourasil dan coelomic fluid dari Lumbricus rubellus sebesar 5 µg/ml, 10 µg/ml dan 20 µg/ml. Kemudian hasil uji MTT dihitung menggunakan rumus persentase kematian sel. Dari hasil perhitungan persentase kematian sel didapatkan bahwa perlakuan 3 (20 µg/ml) dapat menghambat pertumbuhan sel paling tinggi. Kemudian data persentase kematian sel digunakan untuk mendapatkan nilai IC50 sebagai nilai penghambatan sebesar 50% terhadap perlakuan. Pada penelitian ini diperoleh nilai IC50 dari ratarata semua perlakuan sebesar 13,496 µg/ml. Data dari uji sitotoksik kombinasi 5- fluorourasil dan coelomic fluid dari Lumbricus rubellus terhadap cell line HT-29 diperiksaamenggunakan metode uji Kruskal Wallis. Pada uji Kruskal Wallis didapatkan data memiliki nilai signifikan (p < 0,05) sehingga data valid untuk digunakan sebagai data penelitian. Berdasarkan penelitianaini, didapatkan kesimpulan bahwa kombinasi 5-fluorourasil dan coelomic fluid dari Lumbricus rubellus mampu meningkatkan efek sitotoksik terhadap cell line HT-29 dengan konsentrasi coelomic fluid 20 µg/ml memiliki efek sitotoksik tertinggi dibanding semua perlakuan dan didapatkan nilai IC50 dari rerata semua perlakuan kombinasi 5-fluorourasil dan coelomic fluid dari Lumbricus rubellus sebesar 13,496 µg/ml.

English Abstract

Colorectal cancer is one type of cancer that occurs in the mucosa of the colon where the disease has a high morbidity and mortality. The combination of 5- fluourouracil and coelomic fluid from Lumbricus rubellus is expected to be one of the alternative combination therapy that can increase the cytotoxic effect on HT- 29 cell line. The cytotoxic test was performed by MTT assay (3-(4,5- Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) by giving combination of 5- fluourouracil and coelomic fluid from Lumbricus rubellus on concetration 5 μg / ml, 10 μg / ml and 20 μg / ml. Then the MTT assay was calculated using the formula of cell death percentage. From the calculated of cell death percentage it was found that treatment 3 with concentration of 20 μg / ml could inhibit the highest cell growth. Then the percentage data of cell death was used to obtain IC50 value as 50% inhibition value to the treatment. In this research, IC50 average of all treatments is 13,496 μg / ml. The combined 5-fluorouracil and coelomic fluid cytotoxic tests of Lumbricus rubellus against HT-29 cell line were examined using Kruskal-Wallis method. In Kruskal Wallis test, the data has significant value (p <0,05) so that the data is valid to be used as research data. Based on this research, it can be concluded that the combination of 5-fluorouracil and coelomic fluid from Lumbricus rubellus can increase the cytotoxic effect on cell line HT-29 with concentration of coelomic fluid 20 μg / ml has the highest cytotoxic effect compared to all treatments and IC50 value from all treatment a combination of 5-fluorouracil and coelomic fluid from Lumbricus rubellus 13.496 μg / ml.

Other Language Abstract

UNSPECIFIED

Item Type: Thesis (Sarjana)
Identification Number: SKR/FK/2018/45/051803468
Uncontrolled Keywords: cell line HT-29, efek sitotoksik, 5-Fluorourasil, coelomic fluid dari Lumbricus rubellus, HT-29 cell line, cytotoxic effect, 5-Flourouracil, coelomic fluid from Lumbricus rubellus
Subjects: 600 Technology (Applied sciences) > 616 Diseases > 616.9 Other disease > 616.99 Tumors and miscellaneous communicable diseases > 616.994 Cancers
Divisions: Fakultas Kedokteran > Pendidikan Dokter
Depositing User: Nur Cholis
URI: http://repository.ub.ac.id/id/eprint/167560
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