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Kurniaraka, Dinan Zahra (2018) Pengaruh Serbuk Alga Merah (Eucheuma Cottonii) Dan Alga Cokelat (Sargassum Sp.) Terhadap Kadar Malondialdehid Dan Superoksida Dismutase Tikus Yang Diinduksi Stres Oksidatif Dengan Diet Tinggi Sukrosa. Sarjana thesis, Universitas Brawijaya.

Indonesian Abstract

Pada dasarnya, manusia membutuhkan pangan sebagai sumber energi berupa karbohidrat, protein, dan lemak untuk dapat beraktivitas. Seiring perkembangan zaman, manusia lebih memilih makanan instan dan cepat saji yang mengandung kadar gula tinggi, total kalori tinggi namun rendah nutrisi. Hal ini menjadi salah satu faktor utama penyebab peningkatan kadar glukosa pada darah di atas nilai normal atau hiperglikemia. Peningkatan gula darah ini dapat menyebabkan peningkatan produksi Reactive Oxygen Species (ROS) sehingga terjadi stres oksidatif yang lebih lanjut menyebabkan inflamasi. Oleh karena itu, dibutuhkan antioksidan alami seperti alga yang terdapat melimpah di Indonesia, namun minim pemanfaatannya. Pada penelitian ini akan diamati efek pemberian serbuk alga merah (Eucheuma cottonii) dan alga cokelat (Sargassum sp.) terhadap stres oksidatif dengan diet tinggi sukrosa meliputi kadar malondialdehid (MDA) serum darah dan kadar enzim superoksida dismutase (SOD). Penelitian dilakukan dengan rancangan Post Test Only Control Group Design. Tikus dibagi empat (4) kelompok perlakuan pakan: pakan standar (tikus normal), pakan tinggi sukrosa (tikus stres oksidatif), pakan tinggi sukrosa + serbuk E. cottonii dan pakan tinggi sukrosa + serbuk Sargassum sp. Perlakuan diberikan selama 28 hari dan pada akhir percobaan, tikus dilakukan euthanasia dan diambil darah dari jantung untuk analisa MDA dan SOD serum. Hasil penelitian menunjukkan bahwa pakan berpengaruh nyata (p<0.05) terhadap kadar MDA dan SOD serum. Kelompok tikus dengan pakan tinggi sukrosa tanpa serbuk alga memiliki kadar MDA paling tinggi (2,49 ± 0,21ng/100 μL) dan kadar SOD paling rendah (21,80 ± 1,33 unit/100 μL). Pemberian pakan tinggi sukrosa + serbuk E. cottonii, dan pakan tinggi sukrosa + serbuk Sargassum sp. menurunkan secara nyata kadar MDA serum masing-masing 2,06 ± 0,25 dan 1,78 ± 0,33 ng/100 dan meningkatkan kadar SOD serum masing-masing 37,00 ± 5,64 dan 41,89 ± 5,46 unit/100 μL. Kadar MDA kelompok tikus yang diberikan pakan standar (normal) adalah yang paling rendah 1,38 ± 0,17 ng/100 μL dan kadar SOD paling tinggi 46,43 ± 4,67 unit/100 μL. Diet tinggi sukrosa dapat memberikan efek stres oksidatif yang ditandai dengan peningkatan kadar MDA dan penurunan kadar SOD pada tikus yang diberikan pakan tinggi sukrosa dibandingkan dengan tikus dengan pakan standar. Alga merah (Eucheuma cottomii) dan alga cokelat (Sargassum sp.) mampu menurunkan kadar MDA dan mempertahankan atau bahkan menaikkan kadar SOD pada tikus yang diinduksi stres oksidatif dengan diet tinggi sukrosa

English Abstract

Humans need food as a source of energy in the form of carbohydrates, proteins, and fats. Modern lifestyle creates circumstances in which humans prefer instant food and fast food that contains high sugar, high calories but low in nutrients. This is one of the main factors causing elevated blood glucose levels above normal or hyperglycemia. The increase blood sugar level trigers glucose autooxidase that can lead to increased production of Reactive Oxygen Species (ROS) resulting in oxidative stress and further causes inflammation. Therefore, natural antioxidants are required to reduce the adverse effect of ROS. Marine algae such as red and brown algae are abundant in Indonesia and a good source of bioactive compounds, but still less utilized. In this study, the effects of red algae powder (Eucheuma cottonii) and brown algae (Sargassum sp.) malondialdehyde (MDA) levels and superoxide dismutase (SOD) enzyme levels in serum of oxidative-stress induced rats with high sucrose diet. Rats were randomly assigned into 4 groups differing in the type of diet: standard diet (normal group), high sucrose diet (stress-oxidative group), high sucrose diet + E. cottonii powder and high sucrose diet + Sargassum sp. powder. The treatment was given for 28 days (4 weeks). At the end of the experiment, blood were collected by cardiac puncture for analysis of MDA and SOD serum. The results showed that both algae powder significantly affected (α=0.05) MDA level in serum of stress oxidative induced rats with high sucrose diet as much as 2,06 ± 0,25 and 1,78 ± 0,33 ng/100 μl respectively. The MDA level were lower than that in the control group of rats induced stress oxidative without given an algae powder (2,49 ± 0,21ng/100 μL) but still higher than that in the control group of normal rats (1,38 ± 0,17 ng/100 μl). Furthermore, both algae powders were able to increase significantly (α=0.05) SOD level in serum of oxidative stress induced rats as much as 37,00 ± 5,64and41,89 ± 5,46unit/100 μl respectively. The SOD level was higher than that in the control group of rats induced stress oxidative without given an algae powder (21,80 ± 1,33 unit/100 μL) but still lower than that in the control group of normal rats (46,43 ± 4,67 unit/100 μl). A high-sucrose diet may cause oxidative stress characterized by elevated MDA levels and decreased SOD levels. Red algae (Eucheuma cottonii) and brown algae (Sargassum sp.) lowered MDA levels and increased SOD levels in oxidaitve stress-induced rats with high-sucrose diet.

Other Language Abstract

UNSPECIFIED

Item Type: Thesis (Sarjana)
Identification Number: SKR/FTP/2018/404/051809546
Uncontrolled Keywords: Alga, diet tinggi sukrosa, kadar MDA dan SOD,/ Algae, High sucrose diet, MDA and SOD levels
Subjects: 600 Technology (Applied sciences) > 615 Pharmacology and therapeutics > 615.3 Organics drugs > 615.32 Drugs derived from plants and mikroorganisms > 615.329 89 Drugs derived from microorganism, fungi, algae (Rhodophyta (Red algae))
Divisions: Fakultas Teknologi Pertanian > Teknologi Hasil Pertanian
Depositing User: Endang Susworini
URI: http://repository.ub.ac.id/id/eprint/164828
Text
Dinan Zahra Kurniaraka.pdf

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