Parnasukma, Mulam Diratna (2018) Efek Perasan Daun dan Tangkai Semanggi Air (Marsilea crenata) sebagai Prevensi Urolithiasis pada Tikus Putih (Rattus norvegicus) Model Hiperglikemia terhadap Ekspresi Tumor Necrosis Factor Alpha (TNFα) Organ Ginjal dan Aktivitas Superoksida Dismutase (SOD). Sarjana thesis, Universitas Brawijaya.
Abstract
Kerusakan organ ginjal akibat hiperglikemia dapat menjadi salah satu faktor resiko terjadinya urolithiasis. Salah satu pencegahan yang dapat dilakukan yaitu dengan memberikan tanaman semanggi air. Semanggi air (Marsilea crenata) memiliki kandungan kalium dan flavonoid, dimana kandungan tersebut dapat berperan sebagai peluruh kristal, antihiperglikemia, antioksidan, dan antiinflamasi. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian perasan daun dan tangkai semanggi air dalam mencegah terjadinya urolithiasis pada hewan model hiperglikemia ditinjau dari ekspresi TNFα pada organ ginjal dan aktivitas SOD. Penelitian ini menggunakan RAL (Rancangan Acak Lengkap) dengan metode eksperimental. Kelompok perlakuan dibagi menjadi 5, yaitu P1 (kontrol positif urolithiasis), P2 (kontrol positif hiperglikemia dan urolithiasis), dan P3, P4, P5 (induksi hiperglikemia dan perasan daun dan tangkai semanggi air dibagi menjadi beberapa konsentrasi, yaitu 20%, 40%, 60% serta induksi urolithiasis). Variabel penelitian berupa ekspresi TNFα dengan metode imunohistokimia dan aktivitas SOD diukur menggunakan Shimadzu UV-visible spectrophotometer UV-1601 yang dianalisis statistika dengan uji one-way ANOVA dan uji lanjutan (Posthoc Test) dengan taraf kepercayaan 95%. Hasil penelitian menunjukkan pemberian perasan daun dan tangkai semanggi air secara signifikan dapat mencegah kenaikan ekspresi TNFα dan meningkatkan aktivitas SOD pada konsentrasi yang paling baik sebesar 60%. Sehingga dapat disimpulkan bahwa perasan daun dan tangkai semanggi memiliki potensi sebagai prevensi urolithiasis pada hewan model hiperglikemia melalui penurunan ekspresi TNFα dan peningkatan aktivitas SOD.
English Abstract
Kidney organ damaged due to hyperglycemia might be one of the factors of risk occurrence of urolithiasis. One of the prevention that can be conducted is to give Marsilea crenata. Marsilea crenata contains potassium and flavonoid which all of those compound acted as set crystals, antihyperglycemic, antioxidant, and anti-inflammatory.This study was aimed to know the influence of the giving Marsilea crenata leaves and stalks juice in preventing the occurrence of urolithiasis in animal model of hyperglycemia in terms of expression of TNFα on kidney organ and activity of SOD. This study used a CRD (Complete Random Design) with the experimental method. Treatment is divided into 5 groups, namely P1 (positive control urolithiasis), P2 (positive control hyperglycemia and urolithiasis), and P3, P4, P5 (induction hyperglycemia and Marsilea crenata leaves and stalks juice divided into several concentration i.e. 20%, 40%, 60% with induction urolithisis. The study variable was TNFα expression with immunohistochemical method and SOD activity was measured using Shimadzu UV-visible spectrophotometer UV-1601 was analyzed statistically with one-way ANOVA test and advanced test (Posthoc Test) with 95% confidence level. The result showed Marsilea crenata leaves and stalks juice can prevent the increase in expression of TNFα and increasing activity of SOD at the best concentration of 60%. It was concluded that Marsilea crenata leaves and stalks juice have effect as prevention of urolithiasis in animal models of hyperglycemia through the decrease in expression of TNFα and increased activity of SOD.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FKH/2018/161/051810190 |
| Uncontrolled Keywords: | Hiperglikemia, urolithiasis, semanggi air, TNFα, SOD.-Hyperglycemia, urolithiasis, Marsilea crenata, TNFα, SOD. |
| Subjects: | 600 Technology (Applied sciences) > 615 Pharmacology and therapeutics > 615.3 Organics drugs > 615.32 Drugs derived from plants and mikroorganisms > 615.327 Drugs derived from spesific plant (Pteridophyta) |
| Divisions: | Fakultas Kedokteran Hewan > Kedokteran Hewan |
| Depositing User: | soegeng sugeng |
| Date Deposited: | 26 Feb 2019 03:25 |
| Last Modified: | 22 Oct 2021 03:47 |
| URI: | http://repository.ub.ac.id/id/eprint/161451 |
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