Khalifa, AwatifAli (2017) The Effect Of Ethanol Extract Of Dates (Phoenix Dactylifera) On Blood Level Of Inf-Y,Il12, And Bacterial Colonies In Liver Found In Mice Infected With Salmonella Typhimurium. Magister thesis, Universitas Brawijaya.

Indonesian Abstract

English Abstract

Typhoid fever is a serious and often fatal disease in developing countries. It is caused by Salmonella Typhi . Obviously, Salmonella Typhimurium infection in mice is widely accepted as an experimental model for typhoid fever in humans. interleukin (IL-12) family of cytokines is potential to treat immune-mediated inflammatory diseases. IL-12 induces interferon-γ (IFN-γ). Date fruit (Phoenix dactilifera) and its constituents show a role in diseases prevention through anti-oxidant, anti-inflammatory, and anti-bacterial activity. This study used 25 mice divided into 5 groups, including control negative (without infection), control positive (infected with S. Typhimurium), treatment group: P1 ( 100mg/kgBW), P2 (200mg/ kgBW), and P3 (400mg/ kgBW). ELISA was used to measure IL-12 and IFN-γ levels and culture was used to measure bacterial colonies in liver. results indicate that IFN-γ levels decreased significantly at dose 400 mg/kgBW compared with C+ (p 0.05), but descriptively, level of IFN-γ increased at dose 100mg/kgBW. In addition, IL-12 levels increased significantly at dose 400 mg/kgBW compared with (p 0.05), but it decreased at dose 100mg/kgBW. bacteria colonies in liver sample of mice induced S.Typhimurium bacteria in positive control group become zero after treatment was givenin form of phoenix dactylifera (p1.100mg/kg, p2. 200mg/kg ,p3. 400mg/kg ) (p0.05). re was no bacteria found in all treatment groups. In conclusion, ethanol extract of Phoenix dactilifera can improve immune response by increasing IL-12 level and decreasing IFN-γ level, as well as inhibiting systemic diseases by killing bacteria colony in liver.

Other Language Abstract


Item Type: Thesis (Magister)
Identification Number: TES/616.927 2/KHA/t/2017/041702317
Subjects: 600 Technology (Applied sciences) > 616 Diseases > 616.9 Other disease
Divisions: S2 / S3 > Magister Ilmu Biomedis, Fakultas Kedokteran
Depositing User: Budi Wahyono
URI: http://repository.ub.ac.id/id/eprint/158449
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